Chemical modification of cysteine residues is a misleading indicator of their status as active site residues in the vitamin K-dependent γ-glutamyl carboxylation reaction

Journal of Biological Chemistry

Volumn 279, Issue 52, 2004, Pages 54079-54087

  Tie, Jian Ke ^a   Jin, Da Yun ^a   Loiselle, David R ^b   Pope, R Marshall ^c   Straight, David L ^a   Stafford, Darrel W ^a,b  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c INVITROGEN CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACIDS; BIOLOGICAL MEMBRANES; CARBOXYLATION; CATALYSIS; ENZYMES; IONIZATION; MASS SPECTROMETRY; MUTAGENESIS; VITAMINS;

CYSTEINE RESIDUES; ENZYMATIC ACTIVITY; LASER DESORPTION; MEMBRANE ENZYMES;

CHEMICAL MODIFICATION;

4 ACETAMIDO 4' ISOTHIOCYANATOSTILBENE 2,2' DISULFONIC ACID; CARBOXYLASE; CYSTEINE; GAMMA GLUTAMYL CARBOXYLASE; PEPTIDE; SODIUM; SODIUM TETRATHIONATE; TETRATHIONIC ACID; UNCLASSIFIED DRUG; VITAMIN K GROUP;

ARTICLE; BINDING AFFINITY; CARBOXYLATION; CHEMICAL MODIFICATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME INACTIVATION; ENZYME MODIFICATION; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROSOME MEMBRANE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN PROCESSING; PROTEIN TERTIARY STRUCTURE; WILD TYPE;

AMINO ACID SEQUENCE; BINDING SITES; CARBON-CARBON LIGASES; CYSTEINE; ENZYME INHIBITORS; MALEIMIDES; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; POINT MUTATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STILBENES; STRUCTURE-ACTIVITY RELATIONSHIP; SULFATES; SULFHYDRYL REAGENTS; SULFONIC ACIDS; TRYPSIN; VITAMIN K;

EID: 11144222572     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M408945200     Document Type: Article

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