Identification of domains within megalomicin and erythromycin polyketide synthase modules responsible for differences in polyketide production levels in Escherichia coli

Biochemistry

Volumn 43, Issue 50, 2004, Pages 15884-15890

  Murli, Sumati ^a   Piagentini, Misty ^a   McDaniel, Robert ^a   Hutchinson, C Richard ^a  

^a KOSAN BIOSCIENCES INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATION ANALYSIS; BIOSYNTHESIS; CONFORMATIONS; METABOLISM; PROTEINS;

AGLYCON; POLYKETIDE BIOSYNTHESIS; TITERS;

ESCHERICHIA COLI;

6 DEOXYERYTHRONOLIDE B; ERYTHROMYCIN POLYKETIDE SYNTHASE; MEGALOMICIN; POLYKETIDE; POLYKETIDE SYNTHASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; BIOSYNTHESIS; COMPARATIVE STUDY; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION;

AMINOGLYCOSIDES; ERYTHROMYCIN; ESCHERICHIA COLI; POLYKETIDE SYNTHASES; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ILEX COOKII; VECTORS;

EID: 10844291744     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0489964     Document Type: Article

References (12)

1. Volchegursky, Y., Hu, Z., Katz, L., and McDaniel, R. (2000) Biosynthesis of the antiparasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea, Mol. Microbiol. 37, 752-762.
2. Pfeifer, B. A., Admiraal, S. J., Gramajo, H., Cane, D. E., and Khosla, C. (2001) Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli, Science 291, 1790-1792.
3. Dayem, L. C., Carney, J. R., Santi, D. V., Pfeifer, B. A., Khosla, C., and Kealey, J. T. (2002) Metabolic engineering of a methylmalonyl-CoA mutase-epimerase pathway for complex polyketide biosynthesis in Escherichia coli, Biochemistry 41, 5193-5201.
4. Murli, S., Kennedy, J., Dayem, L. C., Carney, J. R., and Kealey, J. T. (2003) Metabolic engineering of Escherichia coli for improved 6-deoxyerythronolide B production, J. Ind. Microbiol. Biotechnol. 30, 500-509.
5. Reid, R., Piagentini, M., Rodriguez, E., Ashley, G., Viswanathan, N., Carney, J., Santi, D. V., Hutchinson, C. R., and McDaniel, R. (2003) A model of structure and catalysis for ketoreductase domains in modular polyketide synthases, Biochemistry 42, 72-79.
6. Hu, Z., Pfeifer, B. A., Chao, E., Murli, S., Kealey, J., Carney, J. R., Ashley, G., Khosla, C., and Hutchinson, C. R. (2003) A specific role of the Saccharopolyspora erythraea thioesterase II gene in the function of modular polyketide synthases, Microbiology 149, 2213-2225.
7. Gokhale, R. S., Tsuji, S. Y., Cane, D. E., and Khosla, C. (1999) Dissecting and exploiting intermodular communication in polyketide synthases, Science 284, 482-485.
8. Kao, C. M., Luo, G., Katz, L., Cane, D. E., and Khosla, C. (1995) Manipulation of macrolide ring size by directed mutagenesis of a modular polyketide synthase, J. Am. Chem. Soc. 117, 9105-9106.
9. Jacobsen, J. R., Hutchinson, C. R., Cane, D. E., and Khosla, C. (1997) Precursor-directed biosynthesis of erythromycin analogues by an engineered polyketide synthase, Science 277, 367-369.
10. Hu, Z., Desai, R. P., Volchegursky, Y., Leaf, T., Woo, E., Licari, P., Santi, D. V., Hutchinson, C. R., and McDaniel, R. (2003) Approaches to stabilization of inter-domain recombination in polyketide synthase gene expression plasmids, J. Ind. Microbiol. Biotechnol. 30, 161-167.
11. Wu, N., Kudo, F., Cane, D. E., and Khosla, C. (2000) Analysis of the molecular recognition features of individual modules derived from the erythromycin polyketide synthase, J. Am. Chem. Soc. 122, 4847-4852.
12. Hans, M., Hornung, A., Dziarnowski, A., Cane, D. E., and Khosla, C. (2003) Mechanistic analysis of acyl transferase domain exchange in polyketide synthase modules, J. Am. Chem. Soc 125, 5366-5374.