A fragment of the envelope protein from dengue-1 virus, fused in two different sites of the meningococcal P64k protein carrier, induces a functional immune response in mice

Biotechnology and Applied Biochemistry

Volumn 39, Issue 1, 2004, Pages 107-114

  Hermida, Lisset ^a   Rodríguez, Rayner ^b   Lazo, Laura ^a   Bernardo, Lídice ^b   Silva, Ricardo ^a   Zulueta, Aída ^a   López, Carlos ^a   Martín, Jorge ^a   Valdés, Iris ^a   Del Rosario, Delfina ^b   Guillén, Gerardo ^a   Guzmán, María G ^b  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

^b INSTITUTO DE MEDICINA TROPICAL PEDRO KOURÍ   (Cuba)

Author keywords

Dengue 1; Fusion; Neutralization; P64k carrier; Protection

Indexed keywords

ANTIGENS; IMMUNOLOGY; PROTEINS; VIRUSES;

FUSION PROTEINS;

BIOCHEMISTRY;

BACTERIAL PROTEIN; ENVELOPE PROTEIN; HYBRID PROTEIN; NEUTRALIZING ANTIBODY; P64K PROTEIN; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VIRUS ANTIBODY;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIGENICITY; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DENGUE; DENGUE VIRUS; DRUG PURIFICATION; FEMALE; FRAGMENTATION REACTION; GENE CONSTRUCT; GENE INSERTION; HETEROLOGOUS EXPRESSION; IMMUNE RESPONSE; IMMUNOGENICITY; INOCULATION; LETHALITY; MOLECULAR RECOGNITION; MOLECULE; MOUSE; NONHUMAN; PLASMID; PROTECTION; PROTEIN ANALYSIS; PURIFICATION; SEROTYPE; VIRUS STRAIN;

ANIMALIA; BACTERIA (MICROORGANISMS); DENGUE VIRUS; DENGUE VIRUS TYPE 2; INSERTION SEQUENCES;

EID: 10744223068     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20030039     Document Type: Article

References (32)

1. Chambers, T. J., Hahn, C. S., Galler, R. and Rice, C. M. (1990) Annu. Rev. Microbiol. 44, 649-688
2. Guzmán, M. G. and Kourí G. (2002) Lancet Infect. Dis. 2, 33-42
3. Brandt, W. E. (1988) J. Infect. Dis. 157, 1105-1111
4. Rice, C. M., Strauss, E. G. and Strauss, J. H. (1986) in Sructure of the Flavivirus Genome (Schlesinger, S. and Schlesinger, M. J., eds.), pp. 279-326, Plenum Press, New York
5. Wang, S. H. E. and Anderson, R. (1999) J. Virol. 73, 2547-2551
6. Staropoli, I., Frenkiel, M. P., Megret, F. and Deubel, V. (1997) Vaccine 15, 1946-1954
7. Sugrue, R. J., Fu, H., Howe, J. and Chan, Y. (1997) J. Virol. 78, 1861-1866
8. Hermida, L., Rodriguez, R., Lazo, L., López, C., Márquez, G., Páez, R., Guzmán, G., Suárez, C., Espinosa, R., García, J. and Guillén, G. (2002) J. Biotechnol. 94, 213-216
9. Srivastava, A. K., Morita, K. and Matsuo, S. (1991) Microbiol. Immunol. 35, 863-870
10. Srivastava, A. K., Putnak, R. J., Warren, R. L. and Hoke, C. H. (1995) Vaccine 13, 1251-1258
11. Simmons, M., Nelson, W. M., Wu, S. J. and Hayes, C. G. (1998) Am. J. Trop. Med. Hyg. 58, 655-662
12. Fonseca, B. A. L., Khoshnood, K., Shope, R. and Mason, P. W. (1991) Am. J. Trop. Med. Hyg. 44, 500-508
13. Hermida, L., Rodríguez, R., Lazo, L., Zulueta, A., López, C., Valdés, I., Silva, R., Chinea, G., Guillén, G., Guzmán, M. G., Sierra, B. and Espinosa, R. (2002) Patent Applic. PCT/Cu02/00006
14. Guillén, G., Álvarez, A., Silva, R., Morera, V., González, S., Musacchio, A., Besada, V., Coizeau, E., Caballero, E., Nazabal, C. et al. (1998) Biotechnol. Appl. Biochem. 27, 189-196
15. Pérez, A., Dickinson, F., Cinza, Z., Ruiz, A., Serrano, T., Sosa, J., González, S., Gutiérrez, Y., Nazábal, C., Gutiérrez, O. et al. (2001) Biotechnol. Appl. Biochem. 34, 121-125
16. González, G., Crombet, T., Catalá, M., Mirabal, V., Hernández, J. C., González, Y., Marinello, P., Guillén, G. and Lage, A. (1998) Ann. Oncol. 9, 431-435
17. Bullock, W. O., Fernández, J. M. and Short, J. M. (1987) Biotechniques 4, 376-378
18. Lacks, S. and Greenberg, J. R. (1977) J. Mol. Biol. 114, 153-168
19. Clarke, D. H. and Casals, J. (1958) Am. J. Trop, Med. Hyg. 7, 561-573
20. Igarashi, A. (1987) J. Gen. Virol. 40, 531-544
21. Rosario, D., Álvarez, A., Vázquez, S., Amin, N., Rodríguez, R., Valdés, K. and Guzmán, M. G. (2001) Biotecnol. Apl. 18, 203-206
22. Sanger, F., Niclen, S. and Coulson, A. R. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5463-5467
23. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) in Molecular Cloning: A Laboratory Manual, 2nd edn. (Irwin, N., Ford, N., Nolan, C., Ferguson, M. and Ockler, M., eds.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY
24. Brown, R., Jarvis, K. and Hyland, K. (1989) Ann. Biochem. 180, 136-139
25. Laemmli, U. K. (1970) Nature (London) 227, 680-685
26. Towbin, H., Staehelin, T. and Gordon, H. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 4350-4354
27. Morens, D. M., Halstead, S. B., Repik, P. M., Putvatana, R. and Raybourne, N. (1985) J. Clin. Microbiol. 22, 250-254
28. 27a Hermida, L., Rodríguez, R., Lazo, L., Silva, R., Zulueta, A., Chinea, G., López, C., Guzmán, M. G. and Guillén, G. (2003) J. Virol. Methods, in the press
29. Simmons, M., Murphy, G. S. and Hayes, C. G. (2001) Am. J. Trop. Med. Hyg. 65, 159-161
30. Falgout, B., Bray, M., Schlesinger, J. and Lai, C. J. (1990) J. Virol. 64, 4356-4363
31. Most, R. G., Murali-Krishna, K., Ahmed, R. and Strauss, J. H. (2000) J. Virol. 74, 8094-8101
32. Most, R. G., Murali-Krishna, K. and Ahmed, R. (2003) Int. Immunol. 15, 119-125