A recombinant envelope protein from Dengue virus purified by IMAC is bioequivalent with its immune-affinity chromatography purified counterpart

Journal of Biotechnology

Volumn 94, Issue 2, 2002, Pages 213-216

  Hermida, L ^a   Rodriguez R ^b   Lazo, L ^a   Lopez C ^a   Marquez G ^a   Paez R ^a   Suarez C ^b   Espinosa, R ^a   Garcia J ^a   Guzman G ^b   Guillen G ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

^b INSTITUTO DE MEDICINA TROPICAL PEDRO KOURÍ   (Cuba)

Author keywords

Dengue; IMAC; Yeast

Indexed keywords

AFFINITY CHROMATOGRAPHY; ANTIBODIES; IMMUNIZATION; PURIFICATION; VIRUSES;

NEUTRALISATION;

PROTEINS;

NEUTRALIZING ANTIBODY; RECOMBINANT PROTEIN; VIRUS ENVELOPE PROTEIN;

ABSORPTION CHROMATOGRAPHY; AFFINITY CHROMATOGRAPHY; ANIMAL EXPERIMENT; ARTICLE; BIOEQUIVALENCE; CONTROLLED STUDY; DENGUE VIRUS; FEMALE; HEMAGGLUTINATION INHIBITION; HUMORAL IMMUNITY; IMMUNOGENICITY; INTERMETHOD COMPARISON; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; VIRUS NEUTRALIZATION; VIRUS TITRATION;

ANIMALS; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; DENGUE VIRUS; FEMALE; HEMAGGLUTINATION INHIBITION TESTS; IMMUNIZATION, PASSIVE; METALS; MICE; MICE, INBRED BALB C; NEUTRALIZATION TESTS; PICHIA; RECOMBINANT PROTEINS; VIRAL ENVELOPE PROTEINS;

DENGUE VIRUS; PICHIA; PICHIA PASTORIS;

EID: 0037187443     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(01)00406-0     Document Type: Article

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