Detection of individual human neutrophil α-defensins (human neutrophil peptides 1, 2 and 3) in unfractionated gingival crevicular fluid - A MALDI-MS approach

Molecular Immunology

Volumn 42, Issue 5, 2005, Pages 575-579

  Lundy, F T ^a   Orr, D F ^b   Shaw, C ^a   Lamey, P J ^a   Linden, G J ^a  

^a QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

^b UNIVERSITY OF ULSTER   (United Kingdom)

Author keywords

Defensin; GCF; MALDI MS; Periodontitis

Indexed keywords

ALPHA DEFENSIN; DEFENSIN 1; DEFENSIN 2; DEFENSIN 3; UNCLASSIFIED DRUG;

ADULT; ARTICLE; CONTROLLED STUDY; FEMALE; GINGIVITIS; HUMAN; MALE; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONISATION MASS SPECTROMETRY; PERIODONTITIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION;

ADULT; AGED; ALPHA-DEFENSINS; AMINO ACID SEQUENCE; CASE-CONTROL STUDIES; FEMALE; GINGIVAL CREVICULAR FLUID; HUMANS; MALE; MIDDLE AGED; MOLECULAR SEQUENCE DATA; NEUTROPHILS; PERIODONTITIS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 10644233169     PISSN: 01615890     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molimm.2004.09.008     Document Type: Article

References (13)

1. J. Ashitani, H. Mukae, T. Hiratsuka, M. Nakazato, K. Kumamoto, and S. Matsukura Elevated levels of alpha-defensins in plasma and BAL fluid of patients with active pulmonary tuberculosis Chest 121 2002 519 526
2. R.P. Darveau, C.M. Belton, R.A. Reife, and R.J. Lamont Local chemokine paralysis, a novel pathogenic mechanism for Porphyromonas gingivalis Infect. Immun. 66 1998 1660 1665
3. T. Ganz Defensins: Antimicrobial peptides of innate immunity Nat. Rev. Immunol. 3 2003 710 720
4. R.J. Genco Pathogenesis and host responses in periodontal disease R.J. Genco H. Goldman D. Cohen Contemporary Periodontics 1990 CV Mosby Company Saint Louis 184 193
5. S. Krisanaprakornkit, A. Weinberg, C.N. Perez, and B.A. Dale Expression of the peptide antibiotic human beta-defensin 1 in cultured gingival epithelial cells and gingival tissue Infect. Immun. 66 1998 4222 4228
6. R.I. Lehrer, T. Ganz, D. Szklarek, and M.E. Selsted Modulation of the in vitro candidacidal activity of human neutrophil defensins by target cell metabolism and divalent cations J. Clin. Invest. 81 1988 1829 1835
7. R.I. Lehrer, A.K. Lichtenstein, and T. Ganz Defensins - antimicrobial and cytotoxic peptides of mammalian cells Ann. Rev. Immunol. 11 1993 105 128
8. F.T. Lundy, D.F. Orr, J.R. Gallagher, P. Maxwell, C. Shaw, S.S. Napier, C.G. Cowan, P.J. Lamey, and J.J. Marley Identification and overexpression of human neutrophil alpha - defensins (human neutrophil peptides 1, 2 and 3) in squamous cell carcinomas of the human tongue Oral Oncol. 40 2004 139 144
9. E.E. Machtei, L.A. Christersson, S.G. Grossi, R. Dunford, J.J. Zambon, and R.J. Genco Clinical criteria for the definition of 'Established Periodontitis' J. Periodontol. 63 1992 207 215
10. M.S. McKay, E. Olson, M.A. Hesla, A. Panyutich, T. Ganz, S. Perkins, and E.F. Rossomando Immunomagnetic recovery of human neutrophil defensins from the human gingival crevice Oral Microbiol. Immunol. 14 1999 190 193
11. Y. Tsutsumi-Ishii, T. Hasebe, and I. Nagaoka Role of CCAAT/enhancer- binding protein site in transcription of human neutrophil peptide-1 and-3 defensin genes J. Immunol. 164 2000 3264 3273
12. T.E. Van Dyke, and C.N. Serhan Resolution of inflammation: a new paradigm for the pathogenesis of periodontal diseases J. Dent. Res. 82 2003 82 90
13. S. van Wetering, P.J. Sterk, K.F. Rabe, and P.S. Hiemstra Defensins: key players or bystanders in infection, injury, and repair in the lung? J. Allergy Clin. Immunol. 104 1999 1131 1138