Differential phosphorylation of connexin46 and connexin50 by H2O2 activation of protein kinase Cγ

Molecular Vision

Volumn 10, Issue , 2004, Pages 688-695

  Lin, Dingbo ^a   Lobell, Samuel ^a   Jewell, Andrea ^a   Takemoto, Dolores J ^a  

^a KANSAS STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAVEOLIN 1; CONNEXIN 46; CONNEXIN 50; GAP JUNCTION PROTEIN; PEROXIDE; PROTEIN KINASE C GAMMA; SERINE; THREONINE; UNCLASSIFIED DRUG; CAV PROTEIN, RAT; CAVEOLIN; EYE PROTEIN; HYDROGEN PEROXIDE; ISOENZYME; PROTEIN KINASE C;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BIOASSAY; CATARACTOGENESIS; CELL ISOLATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; FEMALE; IMMUNOBLOTTING; IMMUNOPRECIPITATION; LENS; LIPID RAFT; MALE; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; RAT; RAT STRAIN; SUCROSE DENSITY GRADIENT CENTRIFUGATION; ANIMAL; DENSITY GRADIENT CENTRIFUGATION; DRUG EFFECT; ENZYMOLOGY; METABOLISM; PHOSPHORYLATION; SPRAGUE DAWLEY RAT; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CAVEOLIN 1; CAVEOLINS; CENTRIFUGATION, DENSITY GRADIENT; CONNEXINS; ENZYME ACTIVATION; EYE PROTEINS; FEMALE; HYDROGEN PEROXIDE; IMMUNOPRECIPITATION; ISOENZYMES; LENS, CRYSTALLINE; MALE; PHOSPHORYLATION; PROTEIN KINASE C; RATS; RATS, SPRAGUE-DAWLEY;

EID: 10644227206     PISSN: 10900535     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (51)

1. Goodenough DA, Goliger JA, Paul DL. Connexins, connexons, and intercellular communication. Annu Rev Biochem 1996; 65:475-502.
2. Donaldson P, Kistler J, Mathias RT. Molecular solutions to mammalian lens transparency. News Physiol Sci 2001; 16:118-23.
3. Zampighi GA, Eskandari S, Kreman M. Epithelial organization of the mammalian lens. Exp Eye Res 2000; 71:415-35.
4. Gong X, Li E, Klier G, Huang Q, Wu Y, Lei H, Kumar NM, Horwitz J, Gilula NB. Disruption of alpha3 connexin gene leads to proteolysis and cataractogenesis in mice. Cell 1997; 91:833-43.
5. White TW, Goodenough DA, Paul DL. Targeted ablation of connexin50 in mice results in microphthalmia and zonular pulverulent cataracts. J Cell Biol 1998; 143:815-25.
6. White TW. Unique and redundant connexin contributions to lens development. Science 2002; 295:319-20.
7. Gerido DA, Sellitto C, Li L, White TW. Genetic background influences cataractogenesis, but not lens growth deficiency, in C×50-knockout mice. Invest Ophthalmol Vis Sci 2003; 44:2669-74.
8. Baruch A, Greenbaum D, Levy ET, Nielsen PA, Gilula NB, Kumar NM, Bogyo M. Defining a link between gap junction communication, proteolysis, and cataract formation. J Biol Chem 2001; 276:28999-9006.
9. Zampighi GA, Loo DD, Kreman M, Eskandari S, Wright EM. Functional and morphological correlates of connexin50 expressed in Xenopus laevis oocytes. J Gen Physiol 1999; 113:507-24.
10. Gaietta G, Deerinck TJ, Adams SR, Bouwer J, Tour O, Laird DW, Sosinsky GE, Tsien RY, Ellisman MH. Multicolor and electron microscopic imaging of connexin trafficking. Science 2002; 296:503-7.
11. Lauf U, Giepmans BN, Lopez P, Braconnot S, Chen SC, Falk MM. Dynamic trafficking and delivery of connexons to the plasma membrane and accretion to gap junctions in living cells. Proc Natl Acad Sci U S A 2002; 99:10446-51.
12. Berthoud VM, Tadros PN, Beyer EC. Connexin and gap junction degradation. Methods 2000; 20:180-7.
13. Musil LS, Le AC, VanSlyke JK, Roberts LM. Regulation of connexin degradation as a mechanism to increase gap junction assembly and function. J Biol Chem 2000; 275:25207-15.
14. Saez JC, Berthoud VM, Branes MC, Martinez AD, Beyer EC. Plasma membrane channels formed by connexins: their regulation and functions. Physiol Rev 2003; 83:1359-400.
15. Oancea E, Meyer T. Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals. Cell 1998; 95:307-18.
16. Shamloo M, Wieloch T. Rapid decline in protein kinase Cgamma levels in the synaptosomal fraction of rat hippocampus after ischemic preconditioning. Neuroreport 1999; 10:931-5.
17. Kolb H, Zhang L, Dekorver L. Differential staining of neurons in the human retina with antibodies to protein kinase C isozymes. Vis Neurosci 1993; 10:341-51.
18. Saleh SM, Takemoto LJ, Zoukhri D, Takemoto DJ. PKC-gamma phosphorylation of connexin 46 in the lens cortex. Mol Vis 2001; 7:240-6 .
19. Saleh SM, Takemoto DJ. Overexpression of protein kinase Cgamma inhibits gap junctional intercellular communication in the lens epithelial cells. Exp Eye Res 2000; 71:99-102.
20. Lin D, Boyle DL, Takemoto DJ. IGF-I-induced phosphorylation of connexin 43 by PKCgamma: regulation of gap junctions in rabbit lens epithelial cells. Invest Ophthalmol Vis Sci 2003; 44:1160-8.
21. Nguyen TA, Boyle DL, Wagner LM, Shinohara T, Takemoto DJ. LEDGF activation of PKC gamma and gap junction disassembly in lens epithelial cells. Exp Eye Res 2003; 76:565-72.
22. Lin D, Zhou J, Zelenka PS, Takemoto DJ. Protein kinase Cgamma regulation of gap junction activity through caveolin-1-containing lipid rafts. Invest Ophthalmol Vis Sci 2003; 44:5259-68.
23. Boscia F, Grattagliano I, Vendemiale G, Micelli-Ferrari T, Altomare E. Protein oxidation and lens opacity in humans. Invest Ophthalmol Vis Sci 2000; 41:2461-5.
24. Spector A, Wang RR, Ma W, Kleiman NJ. Development and characterization of an H2O2-resistant immortal lens epithelial cell line. Invest Ophthalmol Vis Sci 2000; 41:832-43.
25. Ma W, Kleiman NJ, Sun F, Li D, Spector A. Peroxide toxicity in conditioned lens epithelial cells - evaluation of multi-defense systems. Exp Eye Res 2003; 77:711-20.
26. Paron I, D'Elia A, D'Ambrosio C, Scaloni A, D'Aurizio F, Prescott A, Damante G, Tell G. A proteomic approach to identify early molecular targets of oxidative stress in human epithelial lens cells. Biochem J 2004; 378:929-37.
27. Zhou J, Fariss RN, Zelenka PS. Synergy of epidermal growth factor and 12(S)-hydroxyeicosatetraenoate on protein kinase C activation in lens epithelial cells. J Biol Chem 2003; 278:5388-98.
28. Volonte D, Galbiati F, Pestell RG, Lisanti MP. Cellular stress induces the tyrosine phosphorylation of caveolin-1 (Tyr(14)) via activation of p38 mitogen-activated protein kinase and c-Src kinase. Evidence for caveolae, the actin cytoskeleton, and focal adhesions as mechanical sensors of osmotic stress. J Biol Chem 2001; 276:8094-103.
29. Newton AC. Protein kinase C: structural and spatial regulation by phosphorylation, cofactors, and macromolecular interactions. Chem Rev 2001; 101:2353-64.
30. Muppidi JR, Siegel RM. Ligand-independent redistribution of Fas (CD95) into lipid rafts mediates clonotypic T cell death. Nat Immunol 2004; 5:182-9.
31. Schubert AL, Schubert W, Spray DC, Lisanti MP. Connexin family members target to lipid raft domains and interact with caveolin-1. Biochemistry 2002; 41:5754-64.
32. Berthoud VM, Beyer EC, Kurata WE, Lau AF, Lampe PD. The gap-junction protein connexin 56 is phosphorylated in the intracellular loop and the carboxy-terminal region. Eur J Biochem 1997; 244:89-97.
33. Cheng HL, Louis CF. Endogenous casein kinase I catalyzes the phosphorylation of the lens fiber cell connexin49. Eur J Biochem 1999; 263:276-86.
34. Cheng HL, Louis CF. Functional effects of casein kinase I-catalyzed phosphorylation on lens cell-to-cell coupling. J Membr Biol 2001; 181:21-30.
35. Yin X, Jedrzejewski PT, Jiang JX. Casein kinase II phosphorylates lens connexin 45.6 and is involved in its degradation. J Biol Chem 2000; 275:6850-6.
36. Lewis S, Karrer J, Saleh S, Chan X, Tan Z, Hua D, McGill J, Pang YP, Fenwick B, Brightman A, Takemoto D. Synthesis and evaluation of novel aldose reductase inhibitors: Effects on lens protein kinase Cgamma. Mol Vis 2001; 7:164-71 .
37. Xu RX, Pawelczyk T, Xia TH, Brown SC. NMR structure of a protein kinase C-gamma phorbol-binding domain and study of protein-lipid micelle interactions. Biochemistry 1997; 36:10709-17.
38. Ananthanarayanan B, Stahelin RV, Digman MA, Cho W. Activation mechanisms of conventional protein kinase C isoforms are determined by the ligand affinity and conformational flexibility of their C1 domains. J Biol Chem 2003; 278:46886-94.
39. Korichneva I, Hoyos B, Chua R, Levi E, Hammerling U. Zinc release from protein kinase C as the common event during activation by lipid second messenger or reactive oxygen. J Biol Chem 2002; 277:44327-31.
40. Knapp LT, Klann E. Superoxide-induced stimulation of protein kinase C via thiol modification and modulation of zinc content. J Biol Chem 2000; 275:24136-45.
41. Cornish KM, Williamson G, Sanderson J. Quercetin metabolism in the lens: role in inhibition of hydrogen peroxide induced cataract. Free Radic Biol Med 2002; 33:63-70.
42. Lin JS, Fitzgerald S, Dong Y, Knight C, Donaldson P, Kistler J. Processing of the gap junction protein connexin50 in the ocular lens is accomplished by calpain. Eur J Cell Biol 1997; 73:141-9.
43. Xu X, Berthoud VM, Beyer EC, Ebihara L. Functional role of the carboxyl terminal domain of human connexin 50 in gap junctional channels. J Membr Biol 2002; 186:101-12.
44. Razani B, Woodman SE, Lisanti MP. Caveolae: from cell biology to animal physiology. Pharmacol Rev 2002; 54:431-67.
45. Rujoi M, Jin J, Borchman D, Tang D, Yappert MC. Isolation and lipid characterization of cholesterol-enriched fractions in cortical and nuclear human lens fibers. Invest Ophthalmol Vis Sci 2003; 44:1634-42.
46. Rong P, Wang X, Niesman I, Wu Y, Benedetti LE, Dunia I, Levy E, Gong X. Disruption of Gja8 (alpha8 connexin) in mice leads to microphthalmia associated with retardation of lens growth and lens fiber maturation. Development 2002; 129:167-74.
47. Manes S, del Real G, Martinez-A C. Pathogens: raft hijackers. Nat Rev Immunol 2003; 3:557-68.
48. Shaw AR, Li L. Exploration of the functional proteome: lessons from lipid rafts. Curr Opin Mol Ther 2003; 5:294-301.
49. Pearson RB, Kemp BE. Protein kinase phosphorylation site sequences and consensus specificity motifs: tabulations. Methods Enzymol 1991; 200:62-81.
50. Lampe PD, TenBroek EM, Burt JM, Kurata WE, Johnson RG, Lau AF. Phosphorylation of connexin43 on serine368 by protein kinase C regulates gap junctional communication. J Cell Biol 2000; 149:1503-12.
51. Zampighi GA. Distribution of connexin50 channels and hemichannels in lens fibers: a structural approach. Cell Commun Adhes 2003; 10:265-70.