Amplification of mRNA populations using aRNA generated from immobilized oligo(dT)-T7 primed cDNA

BioTechniques

Volumn 20, Issue 4, 1996, Pages 584-591

  Eberwine, James ^a  

^a HOSPITAL OF THE UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DNA POLYMERASE; MESSENGER RNA; RNA POLYMERASE;

ARTICLE; DNA BINDING; DNA TEMPLATE; ENZYME ACTIVITY; GENE AMPLIFICATION; MESSENGER RNA SYNTHESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

BASE SEQUENCE; BIOTECHNOLOGY; DNA PRIMERS; DNA, COMPLEMENTARY; MAGNETICS; MICROSPHERES; MOLECULAR SEQUENCE DATA; RNA, ANTISENSE; RNA, MESSENGER;

EID: 0029939983     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. Aviv, H. and P. Leder. 1972. Purification of biologically active globin messenger RNA by chromatography on oligo-thymidylic acid-cellulose. Proc. Natl. Acad. Sci. USA 69:1408-1411.
2. Eberwine, J., H. Yeh, K. Miyashiro, Y. Cao, S. Nair, R. Finnell, M. Zettel and P. Coleman. 1992. Analysis of gene expression in single live neurons. Proc. Natl. Acad. Sci. USA 89:3010-3014.
3. Edmonds, M., M. Vaughan and H. Nakazato. 1971. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. Proc. Natl. Acad. Sci. USA 68:1336-1340.
4. Hornes, E. and L. Korsnes. 1990. Magnetic DNA hybridization properties of oligonucleotide probes attached to superparamagnetic beads and their use in the isolation of poly(A) mRNA from eucaryotic cells. Genet. Anal. Tech. Appl. 7:145.
5. Jakobsen, K., E. Breivold and E. Hornes. 1990. Purification of mRNA directly from crude plant tissues in 15 minutes using magnetic oligo-dT microspheres. Nucleic Acids Res. 18:3669.
6. Mackler, S., B. Brooks and J. Eberwine. 1992. Stimulus-induced coordinate changes in mRNA abundance in single post-synaptic hippocampal CA1 neurons. Neuron 9:539-548.
7. Miyashiro, K., M. Dichter and J. Eberwine. 1994. On the nature and distribution of mRNAs in hippocampal neuntes: implications for neuronal functioning. Proc. Natl. Acad. Sci. USA 91:10800-10804.