Quantitative real-time polymerase chain reaction based on single copy gene sequence for detection of periodontal pathogens

Journal of Clinical Periodontology

Volumn 31, Issue 12, 2004, Pages 1054-1060

  Morillo, Juan Manuel ^a   Lau, Laura ^a   Sanz, Mariano ^a,b,c   Herrera, David ^a,b   Martín, Conchita ^c   Silva, Augusto ^d  

^a Laboratory of Microbiology   (Spain)

^b Section of Graduate Periodontology   (Spain)

^c UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^d CENTRO DE BIOLOGÍA MOLECULAR SEVERO OCHOA   (Spain)

Author keywords

Actinobacillus actinomycetemcomitans; bspA antigen; Gingipain; Leukotoxin; Periodontitis; Porphyromonas gingivalis; Real time polymerase chain reaction; Tannerella forsythensis; TaqMan

Indexed keywords

BACTERIAL DNA; BUFFER; EDETIC ACID;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; AMPLICON; ANALYTIC METHOD; ARGININE GINGIPAIN GENE; ARTICLE; BACTERIAL CELL; BACTERIAL COUNT; BACTERIAL STRAIN; BACTERIUM; BACTERIUM CULTURE; BSPA ANTIGEN GENE; CALCULATION; CELL SUSPENSION; CONTROLLED STUDY; CORRELATION COEFFICIENT; DIAGNOSTIC PROCEDURE; DNA EXTRACTION; GENE; GENE SEQUENCE; GINGIVA; LKTC GENE; MICROSCOPY; MODEL; NONHUMAN; PATHOGENESIS; PERIODONTAL DISEASE; PORPHYROMONAS GINGIVALIS; QUANTITATIVE ANALYSIS; RANDOMIZATION; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REPRODUCIBILITY; SAMPLE; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; TANNERELLA FORSYTHENSIS; TECHNOLOGY; TIME; TOOTH PLAQUE;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; BACTEROIDES; COLONY COUNT, MICROBIAL; DNA PRIMERS; DNA PROBES; DNA, BACTERIAL; HUMANS; PERIODONTAL DISEASES; POLYMERASE CHAIN REACTION; PORPHYROMONAS GINGIVALIS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 10444223986     PISSN: 03036979     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1600-051X.2004.00608.x     Document Type: Article

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