Tagged library approach to chemical genomics and proteomics

Current Opinion in Chemical Biology

Volumn 8, Issue 1, 2004, Pages 26-32

  Mitsopoulos, Gus ^a   Walsh, Daniel P ^a   Chang, Young Tae ^a  

^a NEW YORK UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LIGAND; PROTEIN;

ANALYTIC METHOD; DOWNSTREAM PROCESSING; GENE LIBRARY; GENETIC SCREENING; GENOMICS; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; PROTEIN TARGETING; PROTEOMICS; REVIEW; STRUCTURE ACTIVITY RELATION;

EID: 1042287126     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbpa.2003.11.002     Document Type: Review

References (21)

1. Peterson R.T., Link B.A., Dowling J.E., Schreiber S.L. Small molecule developmental screens reveal the logic and timing of vertebrate development. Proc Natl Acad Sci USA. 97:2000;12965-12969.
2. Hergenrother P.J., Depew K.M., Schreiber S.L. Small-molecule microarrays: covalent attachment and screening of alcohol-containing small molecules on glass slides. J Am Chem Soc. 122:2000;7849-7850.
3. Barnes-Seeman D., Park S.B., Koehler A.N., Schreiber S.L. Expanding the functional group compatibility of small-molecule microarrays: discovery of novel calmodulin ligands. Angew Chem Int Ed Engl. 42:2003;2376-2379.
4. Tsien R.Y. The green fluorescent protein. Annu Rev Biochem. 67:1998;509-544.
5. Zhang J., Campbell R.E., Ting A.Y., Tsien R.Y. Creating new fluorescent probes for cell biology. Nat Rev Mol Cell Biol. 3:2002;906-918.
6. Roberts R., Szostak J. RNA-peptide fusions for the in vitro selection of peptides and proteins. Proc Natl Acad Sci USA. 94:1997;12297-12302.
7. Wilson D.S., Keefe A.D., Szostak J.W. The use of mRNA display to select high-affinity protein-binding peptides. Proc Natl Acad Sci USA. 98:2001;3750-3755.
8. Hammond P.W., Alpin J., Rise C.E., Wright M., Kreider B.L. In vitro selection and characterization of Bcl-XL-binding proteins from a mix of tissue-specific mRNA display libraries. J Biol Chem. 276:2001;20898-20906.
9. Nielsen P.E., Egholm M., Berg R.H., Buchardt O. Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide. Science. 254:1991;1497-1500.
10. Winssinger N., Harris J.L., Backes B.J., Schultz P.G. From split-pool libraries to spatially addressable microarrays. Angew Chem Int Ed Engl. 40:2001;3083-3085 Reports the first synthesis of a PNA-encoded library of small molecules and its successful use in finding novel cathepsin L inhibitors.
11. Winssinger N., Ficaro S., Schultz P., Harris J.L. Profiling protein function with small molecule micro arrays. Proc Natl Acad Sci USA. 99:2002;11139-11144 The potential of the PNA-tagged small-molecule library is demonstrated by the detection and characterization of caspase activation at the onset of apoptosis and the inhibition of the caspase-executed apoptosis using the small-molecule inhibitor identified in the spatially addressable microarray.
12. Kolb H.C., Finn M.G., Sharpless K.B. Click chemistry: diverse chemical function from a few good reactions. Angew Chem Int Ed Engl. 40:2001;2004-2021 A comprehensive review of click chemistry by its originators describing the philosophy behind the synthetic strategy and detailing the synthesis of several complex molecules.
13. Lewis W.J., Green L.G., Grynszpan F., Radic Z., Carlier P.R., Taylor P., Finn M.G., Sharpless K.B. Click chemistry in situ: acetylcholinesterase as a reaction vessel for the selective assembly of a femtomolar inhibitor from an array of building blocks. Angew Chem Int Ed Engl. 41:2002;1053-1057 A powerful application of click chemistry, utilizing target-directed probe synthesis in which the enzyme target, acetylcholinesterase, selects the molecular building blocks from a click chemistry library in a successful drug discovery application.
14. Löber S., Rodriguez-Loaiza P., Gmeiner P. Click linker: construction of a new family of synthetic resins by 1,3-dipolar cycloaddition. Org Lett. 5:2003;1753-1755.
15. Mitchell M.L., Huang S.J., Fokin V.V., Sharpless K.B., Wong C.H. A potent and highly selective inhibitor of human alpha-1,3-fucosyltransferase via click chemistry. J Am Chem Soc. 125:2003;9588-9589.
16. Khersonsky S.M., Jung D.W., Kang T.W., Walsh D.P., Moon H.S., Jo H., Jacobson E.M., Shetty V., Neubert T.A., Chang Y.T. Facilitated forward chemical genomics using tagged triazine library and zebrafish embryo screening. J Am Chem Soc. 125:2003;11804-11805 Presents an exciting, practical and model application of the tagged library approach for forward chemical genomics. A thorough and complete study from the discovery of a novel small-molecule binder through in vivo embryonic screening to a full characterization of the ligand's protein complement.
17. Moon H.S., Jacobson E.M., Khersonsky S.M., Luzung M.R., Walsh D.P., Xiong W., Lee J.W., Parikh P.B., Lam J.C., Kang T.W. et al. A novel microtubule destabilizing entity from orthogonal synthesis of triazine library and zebrafish embryo screening. J Am Chem Soc. 124:2002;11608-11609.
18. Peterson R.T., Link B.A., Dowling J.E., Schreiber S.L. Small molecule developmental screens reveal the logic and timing of vertebrate development. Proc Natl Acad Sci USA. 97:2000;12965-12969.
19. Rosania G.R., Lee J.W., Ding L., Yoon H.S., Chang Y.T. Combinatorial approach to organelle-targeted fluorescent library based on the styryl scaffold. J Am Chem Soc. 125:2003;1130-1131 Excellent application of a fluorescent tagged library approach in which spectroscopic diversity is maximized through combinatorial synthesis. Discovered is a new class of novel cell-permeable organelle-specific fluorescent dyes that hold great opportunity in directed compound delivery and cellular molecular action studies.
20. Bereiter-Hahn J., Seipel K.H., Voth M., Ploem J.S. Fluorimetry of mitochondria in cells vitally stained with DASPMI or rhodamine 6 GO. Cell Biochem Funct. 1:1983;147-155.
21. Bereiter-Hahn J. Dimethylaminostyrylmethylpyridiniumiodide (DASPMI) as a fluorescent probe for mitochondria in situ. Biochim Biophys Acta. 423:1976;1-14.