Small-molecule microarrays: Covalent attachment and screening of alcohol-containing small molecules on glass slides [20]

Journal of the American Chemical Society

Volumn 122, Issue 32, 2000, Pages 7849-7850

  Hergenrother, Paul J ^a   Depew, Kristopher M ^a   Schreiber, Stuart L ^a  

^a HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL DERIVATIVE; GLASS;

CHEMICAL STRUCTURE; COVALENT BOND; LETTER; MOLECULAR SIZE; REACTION ANALYSIS; STRUCTURE ANALYSIS;

EID: 0034674919     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0014032     Document Type: Letter

References (16)

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6. 2/DMF gave the best results.
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12. (a) Hattori, K.; Schreiber, S. L. Unpublished results.
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14. Although robotic arrayers can print > 130 identical microarrays in a single run, if desired the used slides can be stripped of protein and re-probed (see Supporting Information).
15. 4 allows for covalent attachment of both primary and secondary alcohol containing small molecules.
16. Kuruvilla, F.; Sternson, S.; Hergenrother, P. J.; Shamji, A.; Schreiber, S. L. Unpublished results. Although the long-term stability of these small-molecule microarrays is unknown, useful results were obtained with them even after 3 months of storage in a desiccator at room temperature.