Two-hybrid search for proteins that interact with Sad1 and Kms1, two membrane-bound components of the spindle pole body in fission yeast

Molecular Genetics and Genomics

Volumn 270, Issue 6, 2004, Pages 449-461

  Miki, F ^a   Kurabayashi, A ^a   Tange, Y ^a   Okazaki, K ^a   Shimanuki, M ^a   Niwa, O ^a  

^a KAZUSA DNA RESEARCH INSTITUTE   (Japan)

Author keywords

Kms1; Sad1; Schizosaccharomyces pombe; Spindle pole body; Two hybrid screening

Indexed keywords

BETA GALACTOSIDASE; CLATHRIN; DYNEIN ADENOSINE TRIPHOSPHATASE; FUNGAL PROTEIN; GENE PRODUCT; HYBRID PROTEIN; KMS1 PROTEIN, S POMBE; NUCLEOPORIN; PROTEIN KMS1; PROTEIN SAD1; SAD1 PROTEIN, S POMBE; SCHIZOSACCHAROMYCES POMBE PROTEIN; SNARE PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; BUDDING; CHROMATIN; COATED VESICLE; COMPLEX FORMATION; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENT ANTIBODY TECHNIQUE; FUNGAL GENETICS; GENE IDENTIFICATION; GENE INTERACTION; GENE LOCATION; GENE MUTATION; GENE OVEREXPRESSION; GENETIC ANALYSIS; GENETIC CODE; GENETIC SCREENING; GENETICS; HIGH TEMPERATURE; MEMBRANE; METABOLISM; MITOSIS SPINDLE; MOLECULAR EVOLUTION; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PHYSIOLOGY; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SCHIZOSACCHAROMYCES; SEQUENCE HOMOLOGY; STRUCTURAL GENE; TWO HYBRID SYSTEM; ULTRASTRUCTURE; YEAST;

BETA-GALACTOSIDASE; BINDING SITES; CONSERVED SEQUENCE; EVOLUTION, MOLECULAR; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GENES, FUNGAL; IN SITU HYBRIDIZATION, FLUORESCENCE; MITOTIC SPINDLE APPARATUS; RECOMBINANT FUSION PROTEINS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS;

SACCHAROMYCETALES; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE;

EID: 1042265578     PISSN: 16174615     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00438-003-0938-8     Document Type: Article

References (75)

1. Adams IR, Kilmartin JV (2000) Spindle pole body duplication: a model for centrosome duplication? Trends Cell Biol 10:329-335
2. Alfa C, Fantes P, Hyams J, McLeod M, Warbrick E (1993) Experiments with fission yeast: a laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
3. Al-Feel W, DeMar JC, Wakil SJ (2003) A Saccharomyces cerevisiae mutant strain defective in acetyl-CoA carboxylase arrests at the G2/M phase of the cell cycle. Proc Nat Acad Sci USA 100:3095-3100
4. Al-Khodairy F, Carr AM (1992) DNA repair mutants defining G2 checkpoint pathways in Schizosaccharomyces pombe. EMBO J 11:1343-1350
5. Bahler J, Wu J-Q, Longtine MS, Shah NG, McKenzie III A, Steever AB, Wach A, Philippsen P, Pringle JR (1998) Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 14:943-951
6. Basi G, Schmid E, Maundrell K (1993) TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility. Gene 123:131-136
7. Boehm M, Bonifacino JS (2002) Genetic analyses of adaptin function from yeast to mammals. Gene 286:175-186
8. Bone N, Millar JB, Toda T, Armstrong J (1998) Regulated vacuole fusion and fission in Schizosaccharomyces pombe: an osmotic response dependent on MAP kinases. Curr Biol 8:135-144
9. Brands A, Ho TH (2002) Function of a plant stress-induced gene, HV A22. Synthetic enhancement screen with its yeast homolog reveals its role in vesicular traffic. Plant Physiol 130:1121-1131
10. Bridge AJ, Morphew M, Bartlett R, Hagan IM (1998) The fission yeast SPB component Cut12 links bipolar spindle formation to mitotic control. Genes Dev 12:927-942
11. Byers B (1981) Cytology of the yeast life cycle. In: Strathern JN, Jones EW, Broach JR (eds) Molecular biology of the yeast Saccharomyces. I. Life cycle and inheritance. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp 59-96
12. Cao Y, Cairns BR, Kornberg RD, Laurent BC (1997) Sfh1p, a component of a novel chromatin-remodeling complex, is required for cell cycle progression. Mol Cell Biol 17:3323-3334
13. Chang L, Gould KL (2000) Sid4p is required to localize components of the septation initiation pathway to the spindle pole body in fission yeast. Proc Natl Acad Sci USA 97:5249-5254
14. Chikashige Y, Ding D-Q, Imai Y, Yamamoto M, Haraguchi T, Hiraoka Y (1997) Meiotic nuclear reorganization: switching the position of centromeres and telomeres in the fission yeast Schizosaccharomyces pombe. EMBO J 16:193-202
15. Craven RA, Griffiths DJF, Sheldrick KS, RAndall RE, Hagan IM, Carr AM (1998) Vectors for the expression of tagged proteins in Schizosaccharomyces pombe. Gene 221:59-68
16. Decottignies A, Zarzov P, Nurse P (2001) In vivo localisation of fission yeast cyclin-dependent kinase cdc2p and cyclin B cdc13p during mitosis and meiosis. J Cell Sci 114:2627-2640
17. Ding R, West RR, Morphew DM, Oakley BR, McIntosh JR (1997) The spindle pole body of Schizosaccharomyces pombe enters and leaves the nuclear envelope as the cell cycle proceeds. Mol Biol Cell 8:1461-1479
18. Ding DQ, Chikashige Y, Haraguchi T, Hiraoka Y (1998) Oscillatory nuclear movement in fission yeast meiotic prophase is driven by astral microtubules, as revealed by continuous observation of chromosomes and microtubules in living cells. J Cell Sci 111:701-712
19. Ding DQ, Tomita Y, Yamamoto A, Chikashige Y, Haraguchi T, Hiraoka Y (2000) Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library. Genes Cells 5:169-190
20. Dreger M, Bengtsson L, Schoneberg T, Otto H, Hucho F (2001) Nuclear envelope proteomics: novel integral membrane proteins of the inner nuclear membrane. Proc Natl. Acad Sci USA 98:11943-11948
21. Esashi F, Yanagida M (1999) Cdc2 phosphorylation of Crb2 is required for reestablishing cell cycle progression after the damage checkpoint. Mol Cell 4:167-174
22. Flory MR, Morphew M, Joseph JD, Means AR, Davis TN (2002) Pcp1p, an Spc110p-related calmodulin target at the centrosome of the fission yeast Schizosaccharomyces pombe. Cell Growth Differ 13:47-58
23. Forsberg SL (1993) Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res 21:2955-2956
24. Funabiki H, Hagan I, Uzawa S, Yanagida M (1993) Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast. J Cell Biol 121:961-976
25. Galway ME, Heckman JW Jr, Schiefelbein JW (1997) Growth and ultrastructure of the Arabidopsis root hair: the rhd3 mutation alters vacuole enlargement and tip growth. Planta 201:209-218
26. Goto B, Okazaki K, Niwa O (2001) Cytoplasmic microtubular system implicated in de novo formation of a Rab1-like orientation of chromosomes in fission yeast. J Cell Sci 114:2427-2435
27. Guertin DA, Venkatram S, Gould KL, McCollum D (2002) Dma1 prevents mitotic exit and cytokinesis by inhibiting the septation initiation network (SIN) Dev Cell 3:779-790
28. Hagan IM, Petersen J (2000) The microtubule organizing centers of Schizosaccharomyces pombe. Curr Topics Dev Biol 49:133-159
29. + associates with the fission yeast spindle pole body and is essential for viability. J Cell Biol 129:1033-1047
30. Hayashi N, Murakami S (2002) STM1, a gene which encodes a guanine quadruplex binding protein, interacts with CDC13 in Saccharomyces cerevisiae. Mol Genet Genomics 267:806-813
31. Hirata A, Shimoda C (1994) Structural modification of spindle pole bodies during meiosis II is essential for the normal formation of ascospores in Schizosaccharomyces pombe: ultrastructural analysis of spo mutants. Yeast 10:173-183
32. Hirata A, Tanaka K (1982) Nuclear behavior during conjugation and meiosis in the fission yeast Schizosaccharomyces pombe. J Gen Appl Microbiol 28:263-274
33. Hochstenbach F, Klis FM, van den Ende H, van Donselaar E, Peters PJ, Klausner RD (1998) Identification of a putative alpha-glucan synthase essential for cell wall construction and morphogenesis in fission yeast. Proc Natl Aca Sci USA 95:9161-9166
34. Hoyt MA (2000) Exit from mitosis: spindle pole power. Cell 102:267-270
35. Ikemoto S, Nakamura T, Kubo M, Shimoda C (2000) S. pombe sporulation-specific coiled-coil protein Spo15p is localized to the spindle pole body and essential for its modification. J Cell Sci 113:545-554
36. Jin QW, Fuchs J, Loidl J (2000) Centromere clustering is a major determinant of yeast interphase nuclear organization. J Cell Sci 113:1903-1912
37. Katayama S, Hirata D, Arellano M, Perez P, Toda T (1999) Fission yeast alpha-glucan synthase Mok1 requires the actin cytoskeleton to localize the sites of growth and plays an essential role in cell morphogenesis downstream of protein kinase C function. J Cell Biol 144:1173-1186
38. Kniola B, O'Toole E, McIntosh JR, Bellone B, Allshire R, Mengarelli S, Hultenby K, Ekwall K (2001) The domain structure of centromeres is conserved from fission yeast to humans. Mol Biol Cell 12:2764-2775
39. Knop M, Strasser K (2000) Role of the spindle pole body of yeast in mediating assembly of the prospore membrane during meiosis. EMBO J 19:3657-3667
40. Lewis MJ, Pelham HR (1996) SNARE-mediated retrograde traffic from the Golgi complex to the endoplasmic reticulum. Cell 85:205-215
41. MacNeill SA, Moreno S, Reynolds N, Nurse P, Fantes PA (1996) The fission yeast Cdc1 protein, a homologue of the small subunit of DNA polymerase delta, binds to Pol3 and Cdc27. EMBO J 15:4613-4628
42. Malone CJ, Fixsen WD, Horvitz HR, Han M (1999) UNC-84 localizes to the nuclear envelope and is required for nuclear migration and anchoring during C. elegans development. Development 126:3171-3181
43. Marelli M, Aitchison JD, Wozniak RW (1998) Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p. J Cell Biol 143:1813-1830
44. Maundrell K (1993) Thiamine-repressible expression vectors prep and pRIP for fission yeast. Gene 123:127-130
45. Miki F, Okazaki K, Shimanuki M, Yamamoto A, Hiraoka Y, Niwa O (2002) The 14-kDa dynein light chain-family protein Dlc1 is required for regular oscillatory nuclear movement and efficient recombination during meiotic prophase in fission yeast. Mol Biol Cell 13:930-946
46. Miyata M, Doi H, Miyata H, Johnson BF (1997) Sexual coflocculation by heterothallic cells of the fission yeast Schizosaccharomyces pombe modulated by medium constituents. Antonie Leeuwenhoek 71:207-215
47. Moreno S, Klar A, Nurse P (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol 194:795-823
48. Moser MJ, Flory MR, Davis TN (1997) Calmodulin localizes to the spindle pole body of Schizosaccharomyces pombe and performs an essential function in chromosome segregation. J Cell Sci 110:1805-1812
49. Niwa O, Shimanuki M, Miki F (2000) Telomere-led bouquet formation facilitates homologous chromosome pairing and restricts ectopic interaction in fission yeast meiosis. EMBO J 19:3831-3840
50. Okamoto CT, McKinney J, Jeng YY (2000) Clathrin in mitotic spindles. Am J Physiol Cell Physiol 279:C369-374
51. Okazaki K, Okayama H, Niwa O (2000) The polyubiquitin gene is essential for meiosis in fission yeast. Exp Cell Res 254:143-152
52. Raff JW (1999) Nuclear migration: the missing (L)UNC? Curr Biol 9:R708-R710
53. Rowley R, Subramani S, Young PG (1992) Checkpoint controls in Schizosaccharomyces pombe: rad1. EMBO J 11:1335-1342
54. +, a gene encoding a protein kinase homolog. Cell 49:559-567
55. Saitoh S, Takahashi K, Nabeshima K, Yamashita Y, Nakaseko Y, Hirata A, Yanagida M (1996) Aberrant mitosis in fission yeast mutants defective in fatty acid synthetase and acetyl CoA corboxylase. J Cell Biol 134:949-961
56. Saka Y, Esashi F, Matsusaka T, Mochida S, Yanagida M (1997) Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with a BRCT motif, with Cut5 and Chk1. Genes Dev 11:3387-3400
57. +, is required for the formation of meiotic prophase-specific nuclear architecture. Mol Gen Genet 254:238-249
58. Sohrmann M, Schmidt S, Hagan I, Simanis V (1998) Asymmetric segregation on spindle poles of the Schizosaccharomyces pombe septum-inducing protein kinase Cdc7p. Genes Dev 12:84-94
59. Sparks CA, Morphew M, McCollum D (1999) Sid2p, a spindle pole body kinase that regulates the onset of cytokinesis. J Cell Biol 146:777-790
60. Starr DA, Hermann GJ, Malone CJ, Fixsen W, Priess JR, Horvitz HR, Han M (2001) unc-83 encodes a novel component of the nuclear envelope and is essential for proper nuclear migration. Development 128:5039-5050
61. Stearns T (1997) Motoring to the finish: kinesin and dynein work together to orient the yeast mitotic spindle. J Cell Biol 138:957-960
62. Stepp JD, Pellicena-Palle A, Hamilton S, Kirchhausen T, Lemmon SK (1995) A late Golgi sorting function for Saccharomyces cerevisiae Apm1p, but not for Apm2p, a second yeast clathrin AP medium chain-related protein. Mol Biol Cell 6:41-58
63. Tanaka H, Tanaka K, Murakami H, Okayama H (1999) Fission yeast cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S-phase completion. Mol Cell Biol 19:1038-1048
64. Tanaka K, Yonekawa T, Kawasaki Y, Kai M, Furuya K, Iwasaki M, Murakami H, Yanagida M, Okayama H (2000) Fission yeast Eso1p is required for establishing sister chromatid cohesion during S phase. Mol Cell Biol 20:3459-3469
65. Tange Y, Niwa O (1995) A selection system for diploid and against haploid cells in Schizosaccharomyces pombe. Mol Gen Genet 248:644-648
66. +, is required for the fusion of nuclear envelopes during karyogamy. J Cell Biol 140:247-258
67. Tange Y, Hirata A, Niwa O (2002) An evolutionarily conserved fission yeast protein, Ned1, implicated in normal nuclear morphology and chromosome stability, interacts with Dis3, Pim1/RCC1 and an essential nucleoporin. J Cell Sci 115:4375-4385
68. Tomlin GC, Morrell JL, Gould KL (2002) The spindle pole body protein Cdc11p links Sid4p to the fission yeast septation initiation network. Mol Biol Cell 13:1203-1214
69. Tran PT, Marsh L, Doye V, Inoue S, Chang F (2001) A mechanism for nuclear positioning in fission yeast based on microtubule pushing. J Cell Biol 153:397-411
70. Trelles-Sticken E, Loidl J, Scherthan H (1999) Bouquet formation in budding yeast: initiation of recombination is not required for meiotic telomere clustering. J Cell Sci 112:651-658
71. Veal EA, Toone WM, Jones N, Morgan BA (2002) Distinct roles for glutathione S-transferases in the oxidative stress response in Schizosaccharomyces pombe. J Biol Chem 277:35523-35531
72. Wang H, Lockwood SK, Hoeltzel MF, Schiefelbein JW (1997) The ROOT HAIR DEFECTIVE3 gene encodes an evolutionary conserved protein with GTP-binding motifs and is required for regulated cell enlargement in Arabidopsis. Genes Dev 11:799-811
73. +: a gene required for cell cycle-dependent spindle pole body anchoring in the nuclear envelope and bipolar spindle formation in Schizosaccharomyces pombe. Mol Biol Cell 9:2839-2855
74. Willson J, Wilson S, Warr N, Watts FZ (1997) Isolation and characterization of the Schizosaccharomyces pombe rhp9 gene: a gene required for the DNA damage checkpoint but not the replication checkpoint. Nucleic Acids Res 25:2138-2146
75. Woods A, Sherwin T, Sasse R, MacRae TH, Baines AJ, Gull K (1989) Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies. J Cell Sci 93:491-50