The template region 5′ of the E-site codon is close to S26 in the human 80S ribosome

Molecular Biology

Volumn 38, Issue 6, 2004, Pages 884-890

  Molotkov, M V ^a   Graifer, D M ^a   Eremina, A V ^a   Ivanov, A V ^a   Laletina, E S ^a   Repkova, M N ^a   Ven'yaminova, A G ^a   Karpova, G G ^a  

^a ENERGY SYSTEMS INSTITUTE   (Russian Federation)

Author keywords

80S ribosome; eukaryotes; mRNA analog; photocrosslinking; ribosomal proteins

Indexed keywords

MESSENGER RNA; NUCLEOTIDE DERIVATIVE; PHENYLALANINE; TRANSFER RNA;

ARTICLE; CODON; CROSS LINKING; IRRADIATION; PHOTOREACTIVITY; PROTEIN MODIFICATION; PROTEIN TARGETING; RIBOSOME; ULTRAVIOLET RADIATION;

EUKARYOTA;

EID: 10344259107     PISSN: 00268933     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:MBIL.0000049867.88620.bb     Document Type: Article

References (23)

1. Yusupova G.Zh., Yusupov M.M., Gate J.H.D., Noller H.F. 2001. The path of messenger RNA through the ribosome. Cell. 106, 233-241.
2. Ramakrishnan V. 2002. Ribosome structure and the mechanism of translation. Cell. 108, 557-572.
3. Graifer D.M., Karpova G.G., Knorre D.G. 2001. Template location in the human ribosome as inferred from the results of affine modification by reactive mRNA analogs. Biokhimiya. 66, 725-744.
4. Demeshkina N., Laletina E., Meschaninova M., Veniyaminova A., Graifer D., Karpova G. 2003. Positioning of mRNA codons with respect to 18S rRNA at the P and E sites of human ribosome. Biochim. Biophys. Acta. 1627, 39-46.
5. Demeshkina N.A., Laletina E.S., Meshchaninova M.I., Repkova M.N., Veniyaminova A.G., Graifer D.M., Karpova G.G. 2003. The surroundings of mRNA codons in the P and E sites of the human ribosome as inferred from photocrosslinking with pUUUGUUU. Mol. Biol. 37, 147-155.
6. Styazhkina V.A., Molotkov M.V., Demeshkina N.A., Bulygin K.N., Graifer D.M., Meshchaninova M.I., Repkova M.N., Veniyaminova A.G., Karpova G.G. 2003. Arrangement of the sense and stop codons of the template in the A site of the human ribosome as inferred from crosslinking with oligonucleotide derivatives. Mol. Biol. 37, 1019-1026.
7. Bulygin K.N., Matasova N.B., Graifer D.M., Ven'yaminova A.G., Yamkovoy V.I., Stahl J., Karpova G.G. 1997. Protein environment of mRNA at the decoding site of 80S ribosomes from human placenta as revealed from affinity labeling with mRNA analogs-derivatives of oligoribonucleotides. Biochim. Biophys. Acta. 1351, 325-332.
8. Takahashi Y., Mitsuma T., Hirayama S., Odani S. 2002. Identification of the ribosomal proteins in the vicinity of globin mRNA in the 40S initiation complex. J. Biochem. 132, 705-711.
9. Repkova M.N., Ivanova T.M., Komarova N.I., Meshchaninova M.I., Kuznetsova M.A., Ven'yaminova A.G. 1999. H-Phosphonate synthesis of oligoribonucleotides containing modified bases: I. Photoreactive oligoribonucleotide derivatives with perfluoroarylazido groups in heterocyclic bases. Bioorg. Khim. 25, 690-701.
10. Veniyaminova A.G., Gorn V.V., Zenkova M.A., Komarova N.I., Repkova M.N. 1990. Automated H-phosphonate synthesis of oligoribonucleotides with the use of the 2′-O-tetrahydropyranyl protective group. Bioorg. Khim. 16, 941-950.
11. 3. Mol. Biol. 31, 144-151.
12. Matasova N.B., Myltseva S.V., Zenkova M.A., Graifer D.M., Vladimirov S.N., Karpova G.G. 1991. Isolation of ribosomal subunits containing intact rRNA from human placenta. Estimation of functional activity of 80S ribosomes. Anal. Biochem. 198, 219-223.
13. Bulygin K.N., Meshchaninova M.I., Ven'yaminova A.G., Graifer D.M., Frolova L.Yu., Karpova G.G. 2003. Highly conserved region 1816-1831 of the 18S ribosomal RNA is close to the first nucleotide of the A-site codon in elongation and termination of translation. Mol. Biol. 37, 486-493.
14. Bulygin K.N., Repkova M.N., Ven'yaminova A.G., Graifer D.M., Karpova G.G., Frolova L.Yu., Kisselev L.L. 2002. Positioning of the mRNA stop signal with respect to polypeptide chain release factors and ribosomal proteins in 80S ribosomes. FEBS Lett. 514, 96-101.
15. Bulygin K.N., Graifer D.M., Repkova M.N., Smolenskaya I.A., Veniyaminova A.G., Karpova G.G. 1997. Nucleotide G-1207 of 18S rRNA is an essential component of the human 80S ribosomal decoding center. RNA. 3, 1480-1485.
16. Madjar J.-J., Arpin M., Buisson M., Reboud J.-P. 1979. Spot position of rat liver ribosomal proteins by four different two-dimensional electrophoresis in polyacrylamide gel, Mol. Gen. Genet. 171, 121-134.
17. Graifer D.M., Lyakhovich A.V., Demeshkina N.A., Ivanov A.V., Repkova M.N., Ven'yaminova A.G., Karpova G.G. 1999. Photoaffinity modification of human 80S ribosomes with an mRNA analog, hexaribonucleotide pUUUGUU derivative carrying an arylazido group on guanine. Mol. Biol. 33, 174-182.
18. Ivanov A.V., Malygin A.A., Karpova G.G. 2003. Interaction of human ribosomal protein S26 with fragments of its own mRNA and pre-mRNA in nuclear extract of HeLa cells. Mol. Biol. 37, 900-905.
19. Morgan D.G., Menetret J.-F., Neuhof A., Rapoport T.A., Akey C.W. 2002. Structure of the mammalian ribosome-channel complex at 17 Å resolution. J. Mol. Biol. 324, 871-886.
20. Wool I.G., Chan Y.-L., Glueck A. 1996. Mammalian ribosomes: The structure and the evolution of the proteins. In Translational Control. Eds. Hershey J.W.B., Matthews M.B., Sonenberg N. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 685-732.
21. Molotkov M.V., Graifer D.M., Meshchaninova M.I., Repkova M.N., Ven'yaminova A.G., Karpova G.G. 2004. Protein environment of the sense codon of the template in the A site of the human ribosome as inferred from crosslinking to oligoribonucleotide derivatives. Mol. Biol. 38, 493-500.
22. Collatz E., Ulbrich N., Tsurugi K., Lightfoot H.N., MacKinlay W., Lin A., Wool I. 1977. Isolation of eukaryotic ribosomal proteins. J. Biol. Chem. 252, 9071-9080.
23. Malygin A.A., Shaulo D.D., Karpova G.G. 2000. Proteins S7, S10, S16, and S19 of the human 40S ribosomal subunit are most resistant to dissociation by salt. Biochim. Biophys. Acta. 1494, 213-216.