The mRNA codon environment at the P and E sites of human ribosomes deduced from photocrosslinking with pUUUGUU derivatives

Molekulyarnaya Biologiya

Volumn 37, Issue 1, 2003, Pages 147-155

  Demeshkina, N A ^a   Laletina, E S ^a   Meshchaninova, M I ^a   Repkova, M N ^a   Ven'yaminova, A G ^a   Graifer, D M ^a   Karpova, G G ^a  

^a INSTITUTE OF CHEMICAL BIOLOGY AND FUNDAMENTAL MEDICINE   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; PHENYLALANINE; RIBONUCLEOTIDE; RIBOSOME RNA; TRANSFER RNA; URIDINE; VALINE; CROSS LINKING REAGENT; PHOSPHORUS; RIBOSOME PROTEIN; RNA 18S; RPS23 PROTEIN, HUMAN; RPS26 PROTEIN, HUMAN;

ARTICLE; CODON; COMPLEX FORMATION; CONTROLLED STUDY; CROSS LINKING; HUMAN; HUMAN TISSUE; IRRADIATION; POSITRON; PROTEIN BINDING; RIBOSOME; ULTRAVIOLET RADIATION; CHEMISTRY; GENETICS; METABOLISM; METHODOLOGY; PHOTOCHEMISTRY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

CODON; CROSS-LINKING REAGENTS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; PHOSPHORUS RADIOISOTOPES; PHOTOCHEMISTRY; RIBOSOMAL PROTEINS; RIBOSOMES; RNA, MESSENGER; RNA, RIBOSOMAL, 18S; ULTRAVIOLET RAYS;

EID: 0038343425     PISSN: 00268984     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Yusupova G.Zh., Yusupov M.M., Cate J.H.D., Noller H.F. 2001. The path of messenger RNA through the ribosome. Cell. 106, 233-241.
2. Bulygin K.N., Graifer D.M., Repkova M.N., Smolenskaya I.A., Veniyaminova A.G., Karpova G.G. 1997. Nucleotide G1207 of 18S rRNA is an essential component of the human 80S ribosomal decoding center. RNA. 3, 1480-1485.
3. Demeshkina N., Repkova M., Ven'yaminova A., Graifer D., Karpova G. 2000. Nucleotides of 18S rRNA surrounding mRNA codons at the human ribosomal A, P and E sites, respectively: a cross-linking study with mRNA analogues carrying aryl azide group at either the uracil or the guanine residue. RNA. 6, 1727-1736.
4. Matasova N.B., Myltseva S.V., Zenkova M.A., Graifer D.M., Vladimirov S.N., Karpova G.G. 1991. Isolation of ribosomal subunits containing intact rRNA from human placenta. Estimation of functional activity of 80S ribosomes. Analyt. Biochem. 198, 219-223.
5. Bulygin K.N., Repkova M.N., Ven'yaminova A.G., Graifer D.M., Karpova G.G., Frolova L.Yu., Kisselev L.L. 2002. Positioning of the mRNA stop signal with respect to polypeptide chain release factors and ribosomal proteins in 80S ribosomes. FEBS Lett. 514, 96-101.
6. Malygin A.A., Dobrikov M.I., Repkova M.N., Shishkin G.V., Veny'aminova A.G., Karpova G.G. 1999. Proteins neighboring 18S rRNA conserved sequences 609-618 and 1047-1061 within the 40S human ribosomal subunit. RNA. 5, 1656-1664.
7. Wollenzien P. 1988. Isolation and identification of RNA cross-links. Methods Enzymol. 164, 319-329.
8. Watanabe S. 1972. Interaction of siomycin with the acceptor site of E. coli ribosomes. J. Mol. Biol. 67, 443-457.
9. Graifer D.M., Nekhai S.Yu., Mundus D.A., Fedorova O.S., Karpova G.G. 1992. Interaction of human and Escherichia coli tRNA with human 80S ribosomes in the presence of oligo- and polyuridylate template. Biochim. Biophys. Acta. 1171, 56-64.
10. Gutell R.R. 1993. Collection of small subunit (16S and 16S-like) ribosomal RNA structures. Nucleic Acids Res. 21, 3051-3054.
11. Clemons W.M., May J.L.C., Wimberly B.T., McCutcheon J.P., Capel M.S., Ramakrishnan V. 1999. Structure of a bacterial 30S ribosomal subunit at 5.5 Å resolution. Nature. 400, 833-840.
12. Sergiev P.V., Lavrik I.N., Wlasoff V.A., Dokudovskaya S.S., Dontsova O.A., Bogdanov A.A., Brimacombe R. 1997. The path of mRNA through the bacterial ribosome: a site-directed cross-linking study using new photoreactive derivatives of guanosine and uridine. RNA. 3, 464-475.
13. Bhangu R., Juzumiene D., Wollenzien P. 1994. Arrangement of messenger RNA on Escherichia coli ribosomes with respect to 10 16S rRNA cross-linking sites. Biochemistry. 33, 3063-3070.