Complete inhibition of acute humoral rejection using regulated expression of membrane-tethered anticoagulants on xenograft endothelium

American Journal of Transplantation

Volumn 4, Issue 12, 2004, Pages 1958-1963

  Chen, Daxin ^a   Weber, Michele ^a   McVey, John H ^b   Kemball Cook, Geoffrey ^b   Tuddenham, Edward G D ^b   Lechler, Robert I ^a   Dorling, Anthony ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b MRC CLINICAL SCIENCES CENTRE   (United Kingdom)

Author keywords

Acute humoral rejection; Anticoagulant; Coagulation; Xenotransplantation

Indexed keywords

ANTICOAGULANT AGENT; CYCLOSPORIN A; HIRUDIN; HYBRID PROTEIN;

ACUTE DISEASE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTICOAGULATION; ARTICLE; CONTROLLED STUDY; ENDOTHELIUM CELL; GENE EXPRESSION REGULATION; GRAFT REJECTION; HUMORAL IMMUNITY; IMMUNE RESPONSE; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; SWINE; T LYMPHOCYTE ACTIVATION; TARGET CELL; VASCULAR ENDOTHELIUM; XENOGRAFT; XENOTRANSPLANTATION;

ANIMALS; ANTIBODY FORMATION; ANTICOAGULANTS; CYCLOSPORINE; ENDOTHELIUM, VASCULAR; FLOW CYTOMETRY; GRAFT REJECTION; HIRUDINS; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; MICE; MICE, INBRED C57BL; PLATELET COUNT; RATS; RATS, INBRED LEW; T-LYMPHOCYTES; THROMBOPLASTIN; TRANSPLANTATION, HETEROLOGOUS;

EID: 10044240662     PISSN: 16006135     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1600-6143.2004.00625.x     Document Type: Article

References (31)

1. Zaidi A, Schmoeckel M, Bhatti F et al. Life-supporting pig-to-primate renal xenotransplantation using genetically modified donors. Transplantation 1998; 65: 1584-1590.
2. Diamond LE, Quinn CM, Martin MJ, Lawson J, Platt JL, Logan JS. A human CD46 transgenic pig model system for the study of discordant xenotransplantation. Transplantation 2001; 71: 132-142.
3. Rose AG, Cooper DK. Venular thrombosis is the key event in the pathogenesis of antibody-mediated cardiac rejection. Xenotransplantation 2000; 7: 31-41.
4. Ierino FL, Kozlowski T, Siegel JB et al. Disseminated intravascular coagulation in association with the delayed rejection of pig-to-baboon renal xenografts. Transplantation 1998; 66: 1439-1450.
5. Cowan PJ, Aminia A, Barlow H et al. Renal xenografts from triple transgenic pigs are not hyperacutely rejected but cause coagulopathy in non-immunosuppressed baboons. Transplantation 2000; 69: 2504-2515.
6. Cozzi E, Simioni P, Boldrin M et al. Alterations in the coagulation profile in renal pig-to-monkey xenotransplantation. Am J Transplant 2004; 4: 335-345.
7. Lin SS, Weidner BC, Byrne GW et al. The role of antibodies in acute vascular rejection of pig-to-baboon cardiac transplants. J Clin Invest 1998; 101: 1745-1756.
8. Kopp CW, Robson SC, Siegel JB et al. Regulation of monocyte tissue factor activity by allogeneic and xenogeneic endothelial cells. Thromb Haemost 1998; 79: 529-538.
9. Nagayasu T, Saadi S, Holzknecht RA, Plummer TB, Platt JL. Expression of tissue factor mRNA in cardiac xenografts: Clues to the pathogenesis of acute vascular rejection. Transplantation 2000; 69: 475-482.
10. Platt JL, Dalmasso AP, Lindman BJ, Ihrcke NS, Bach FH. The role of C5a and antibody in the release of heparan sulfate from endothelial cells. Eur J Immunol 1991; 21: 2887-2890.
11. Siegel JB, Grey ST, Lesnikoski BA et al. Xenogeneic endothelial cells activate human prothrombin. Transplantation 1997; 64:888-896.
12. Robson SC, Kaczmarek E, Siegel JB et al. Loss of ATP diphosphohydrolase activity with endothelial cell activation. J Exp Med 1997; 185: 153-163.
13. Robson SC, Cooper DK, d'Apice AJ. Disordered regulation of coagulation and platelet activation in xenotransplantation. Xenotransplantation 2000; 7: 166-176.
14. Kopp CW, Siegel JB, Hancock WW et al. Effect of porcine endothelial tissue factor pathway inhibitor on human coagulation factors. Transplantation 1997; 63: 749-758.
15. Pareti FI, Mazzucato M, Bottini E, Mannucci PM. Interaction of porcine von Willebrand factor with the platelet glycoproteins Ib and IIb/IIIa complex. Br J Haematol 1992; 82: 81-86.
16. Dorling A. Are anti-endothelial cell antibodies a pre-requisite for the acute vascular rejection of xenografts? Xenotransplantation 2003; 10: 16-23.
17. Palmetshofer A, Galili U, Dalmasso AP, Robson SC, Bach FH. Alpha-galactosyl epitope-mediated activation of porcine aortic endothelial cells: Type II activation. Transplantation 1998; 65: 971-978.
18. Morigi M, Zoja C, Colleoni S et al. Xenogeneic serum promotes leukocyte-endothelium interaction under flow through two temporally distinct pathways: Role of complement and nuclear factor-kappaB. J Am Soc Nephrol 1999; 10: 2197-2207.
19. Xu H, Yin D, Naziruddin B et al. The in vitro and in vivo effects of anti-galactose antibodies on endothelial cell activation and xenograft rejection. J Immunol 2003; 170: 1531-1539.
20. Cowan PJ, Aminian A, Barlow H et al. Protective effects of recombinant human antithrombin III in pig-to-primate renal xenotransplantation. Am J Transplant 2002; 2: 520-525.
21. Robson SC, Young VK, Cook NS et al. Thrombin inhibition in an ex-vivo model of porcine heart xenograft hyperacute rejection. Transplantation 1996; 61: 862-868.
22. Cruzado JM, Riera TM, Lloberas N et al. Effect of platelet-activating factor (PAF) receptor antagonist on hyperacute xenograft rejection: Evaluation in a pig kidney-human blood xenoperfusion model. Clin Exp Immunol 1998; 113: 136-144.
23. Lesnikoski B, Candinas D, Otschu I, Metternitch R, Bach FH, Robson SC. Thrombin inhibition in discordant xenograft rejection. Xenotransplantation 1997; 4: 140-146.
24. Koyamada N, Miyatake T, Candinas D et al. Apyrase administration prolongs discordant xenograft survival. Transplantation 1996; 62: 1739-1743.
25. Dorling A. Genetically engineering a pig that minimizes coagulation incompatibilities. Graft 2001; 4: 72-75.
26. Riesbeck K, Dorling A, Kemball-Cook G et al. Human tissue factor pathway inhibitor fused to CD4 binds both FXa and TF/FVIIa at the cell surface. Thromb Haemost 1997; 78: 1488-1494.
27. Riesbeck K, Chen D, Kemball-Cook G et al. Expression of hirudin fusion proteins in mammalian cells: A strategy for prevention of intravascular thrombosis. Circulation 1998; 98: 2744-2752.
28. Chen D, Riesbeck K, McVey JH et al. Regulated inhibition of coagulation by porcine enclothelial cells expressing P-selectin-tagged hirudin and TFPI fusion proteins. Transplantation 1999; 68: 832-839.
29. Chen D, Giannopoulos K, Shiels PG et al. Inhibition of intravascular thrombosis in murine endotoxaemia by targeted expression of hirudin and tissue factor pathway inhibitor analogues to activated endothelium. Blood First Edition Paper 2004; prepublished online May 4: DOI 10.1182/blood-2003-12-4365.
30. Soares MP, Lin Y, Anrather J et al. Expression of heme oxygenase-1 can determine cardiac xenograft survival. Nat Med 1998; 4: 1073-1077.
31. Koyamada N, Miyatake T, Candinas D et al. Transient complement inhibition plus T-cell immunosuppression induces long-term survival of mouse-to-rat cardiac xenografts. Transplantation 1998; 65: 1210-1215.