Probing the conformation of NhaA, a Na+/h+ antiporter from Escherichia coli, with trypsin

Biochemistry

Volumn 36, Issue 47, 1997, Pages 14572-14576

  Rothman, Andrea ^a   Gerchman, Yoram ^a   Padan, Etana ^a   Schuldiner, Shimon ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIPORTER; ANTIPORTER NHAA; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; CONFORMATIONAL TRANSITION; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME DEGRADATION; ESCHERICHIA COLI K 12; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN CONFORMATION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CELL MEMBRANE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SODIUM-HYDROGEN ANTIPORTER; TRYPSIN;

EID: 0030726673     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi971800y     Document Type: Article

References (22)

1. Padan, E., and Schuldiner, S. (1996) in Handbook of Biological Physics (Konings, W. N., Kaback, H. R., and Lolkema, J. S., Eds.) pp 501-531, Elsevier Science, Amsterdam, The Netherlands.
2. Karpel, R., Alon, T., Glaser, G., Schuldiner, S., and Padan, E. (1991) J. Biol. Chem. 266, 21753-21759.
3. Taglicht, D., Padan, E., and Schuldiner, S. (1991) J. Biol. Chem. 266, 11289-11294.
4. Rothman, A., Padan, E., and Schuldiner, S. (1996) J. Biol. Chem. 271, 32288-32292.
5. Gerchman, Y., Olami, Y., Rimon, A., Taglicht, D., Schuldiner, S., and Padan, E. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 1212-1216.
6. Rimon, A., Gerchman, Y., Olami, Y., Schuldiner, S., and Padan, E. (1995) J. Biol. Chem. 270, 26813-26817.
7. Jorgensen, P. L. (1983) Ciba Found. Symp. 95, 253-272.
8. Karlish, S., Goldshleger, R., and Stein, W. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 4566-4570.
9. Grunewald, M., and Kanner, B. (1995) J. Biol. Chem. 270, 17017-17024.
10. Frillingos, S., and Kaback, H. R. (1996) Biochemistry 35, 3950-3956.
11. Frillingos, S., Wu, J., Venkatesan, P., and Kaback, R. H. (1997) Biochemistry 36, 6408-6414.
12. Goldberg, E. B., Arbel, T., Chen, J., Karpel, R., Mackie, G. A., Schuldiner, S., and Padan, E. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 2615-2619.
13. Karpel, R., Olami, Y., Taglicht, D., Schuldiner, S., and Padan, E. (1988) J. Biol. Chem. 263, 10408-10414.
14. Tabor, S., and Richardson, C. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 1074-1078.
15. Rosen, B. P. (1986) Methods Enzymol. 125, 328-336.
16. Kaback, H. R., and Barnes, E. M. (1971) J. Biol. Chem. 246, 5523-5531.
17. Bradford, W. (1976) Anal. Biochem. 72, 248-254.
18. Schagger, H., and von Jagow, G. (1987) Anal. Biochem. 166, 368-379.
19. King, S., Otter, T., and Witman, G. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 4717-4721.
20. Hummel, B. C. W. (1959) Can. J. Biochem. Physiol. 37, 1393.
21. Wakabayashi, S., Shigekawa, M., and Pouyssegur, J. (1997) Physiol. Rev. 77, 51-74.
22. Sekler, I., Kobayashi, S., and Kopito, R. R. (1996) Cell 86, 929-935.