Detection of intracellular phosphorylated ERK1/2 in natural killer cells by flow cytometry

Haematologica

Volumn 89, Issue 1, 2004, Pages 101-103

  Wang, Yanyan ^a,b   Liang, Shujuan ^a   Zheng, Xiaodong ^a,b   Wei, Haiming ^a   Sun, Rui ^a,b   Tian, Zhigang ^a,b  

^a UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

^b SHANDONG PROVINCIAL HOSPITAL AFFILIATED TO SHANDONG FIRST MEDICAL UNIVERSITY   (China)

Author keywords

ERK molecules; Flow cytometry; Interleukin 15; NK cells; Phosphorylation

Indexed keywords

CD56 ANTIGEN; MITOGEN ACTIVATED PROTEIN KINASE 1; RECOMBINANT INTERLEUKIN 15; RECOMBINANT INTERLEUKIN 2;

ARTICLE; CONTROLLED STUDY; DOSE RESPONSE; ENZYME PHOSPHORYLATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNE SYSTEM; NATURAL KILLER CELL; SIGNAL TRANSDUCTION; T LYMPHOCYTE SUBPOPULATION;

FLOW CYTOMETRY; HUMANS; KILLER CELLS, NATURAL; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; PHOSPHORYLATION;

EID: 0842306980     PISSN: 03906078     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Biron CA. Activation and function of natural killer cell responses during viral infections. Curr Opin Immunol 1997;9:24-34.
2. Fehniger TA, Caligiuri MA. Interleukin 15: biology and relevance to human disease. Blood 2001;97:14-32.
3. Miyazaki T, Liu ZJ, Kawahara A, Minami Y, Yamada K, Tsujimoto Y, et al. Three distinct IL-2 signaling pathways mediated by bcl-2, c-myc, and Ick cooperate in hematopoietic cell proliferation. Cell 1995;81:223-31.
4. Abe MK, Kuo WL, Hershenson MB, Rosner MR. Extracellular signal-regulated kinase 7 (ERK7), a novel ERK with a C-terminal domain that regulates its activity, its cellular localization, and cell growth. Mol Cell Biol 1999;19:1301-12.
5. Fleisher TA, Dorman SE, Anderson JA, Vail M, Brown MR, Holland SM. Detection of intracellular phosphorylated STAT-1 by flow cytometry. Clin Immunol 1999;90:425-30.
6. Uzel G, Frucht DM, Fleisher TA, Holland SM. Detection of intracellular phosphorylated STAT-4 by flow cytometry. Clin Immunol 2001;100:270-6.
7. Tazzari PL, Cappellini A, Bortul R, Ricci F, Billi AM, Tabellini G, et al. Flow cytometric detection of total and serine 473 phosphorylated Akt. J Cell Biochem 2002;86:704-15.
8. Perez OD, Nolan GP. Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry. Nat Biotechnol 2002;20:155-62.
9. Pala P, Hussell T, Openshaw PJ. Flow cytometric measurement of intracellular cytokines. J Immunol Methods 2000;243:107-24.
10. Ge X, Fu YM, Meadows GG. UO126, a mitogen-activated protein kinase kinase inhibitor, inhibits the invasion of human A375 melanoma cells. Cancer Lett 2002;179:133-40.