Detection of intracellular phosphorylated STAT-4 by flow cytometry

Clinical Immunology

Volumn 100, Issue 3, 2001, Pages 270-276

  Uzel, Gülbûl ^a   Frucht, David M ^a   Fleisher, Thomas A ^a   Holland, Steven M ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA INTERFERON; INTERLEUKIN 12; POLYCLONAL ANTIBODY; STAT4 PROTEIN;

ARTICLE; B LYMPHOCYTE; BLAST CELL; CONTROLLED STUDY; DEPHOSPHORYLATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LYMPHOCYTE ACTIVATION; MYCOBACTERIOSIS; NATURAL KILLER CELL; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; T LYMPHOCYTE;

EID: 0034874691     PISSN: 15216616     EISSN: None     Source Type: Journal    
DOI: 10.1006/clim.2001.5078     Document Type: Article

References (24)

1. IL-12: A key cytokine in immune regulation
2. The function of type I interferons in antimicrobial immunity
3. IL-12: Initiation cytokine for cell-mediated immunity
4. Expression cloning of a human IL-12 receptor component: A new member of the cytokine receptor superfamily with strong homology to gp130
5. A functional interleukin 12 receptor complex is composed of two beta- type cytokine receptor subunits
6. Stat4 regulates multiple components of IFN-gamma-inducing signaling pathways
7. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins
8. Interleukin 12 induces tyrosine phosphorylation and activation of STAT4 in human lymphocytes
9. Recruitment of Stat4 to the human interferon-alpha/beta receptor requires activated Stat2
10. Th1 T-cell and monocyte defects
11. Inherited interleukin 12 deficiency in a child with bacille Calmette-Guerin and Salmonella enteritidis disseminated infection
12. Impairment of mycobacterial immunity in human interleukin-12 receptor deficiency
13. Severe mycobacterial and Salmonella infections in interleukin-12 receptor-deficient patients
14. IL-12 and IFN-gamma in host defense against mycobacteria and salmonella in mice and men
15. IL-12-independent costimulation pathways for interferon-gamma production in familial disseminated Mycobacterium avium complex infection
16. Residual type 1 immunity in patients genetically deficient for interleukin 12 receptor beta1 (IL-12Rbeta1): Evidence for an il- 12rbeta1-independent pathway of il-12 responsiveness in human t cells
17. Measurement of T-cell CD69 expression: A rapid and efficient means to assess mitogen- or antigen-induced proliferative capacity in normals
18. Impaired IL-12 responses and enhanced development of Th2 cells in Stat4-deficient mice
19. Inducible expression of Stat4 in dendritic cells and macrophages and its critical role in innate and adaptive immune responses
20. Stat4 is expressed in activated peripheral blood monocytes, dendritic cells, and macrophages at sites of Th1-mediated inflammation
21. Role of the Stat4 N domain in receptor proximal tyrosine phosphorylation
22. Detection of intracellular phosphorylated STAT-1 by flow cytometry
23. Interleukin-2 enhances the response of natural killer cells to interleukin-12 through up-regulation of the interleukin-12 receptor and STAT4
24. Inhibition of Th1 immune response by glucocorticoids: Dexamethasone selectively inhibits IL-12-induced Stat4 phosphorylation in T lymphocytes