Inhibition of hepatitis B virus replication by drug-induced depletion of nucleocapsids

Science

Volumn 299, Issue 5608, 2003, Pages 893-896

  Deres, Karl ^a   Schröder, Claus H ^b   Paessens, Arnold ^a   Goldmann, Siegfried ^a   Hacker, Hans Jörg ^b   Weber, Olaf ^c   Krämer, Thomas ^a   Niewöhner, Ulrich ^a   Pleiss, Ulrich ^a   Stoltefuss, Jürgen ^a   Graef, Erwin ^a   Koletzki, Diana ^a   Masantschek, Ralf N A ^a   Reimann, Anja ^b   Jaeger, Rainer ^a   Groß, Rainer ^a   Beckermann, Bernhard ^a   Schlemmer, Karl Heinz ^a   Haebich, Dieter ^a  

^a BAYER PHARMA AG   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^c BAYER CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DRUG PRODUCTS; INTERFERONS; VIRUSES;

INFECTION;

DISEASES;

ADEFOVIR; ALPHA INTERFERON; BAY 38 7690; BAY 39 5493; BAY 41 4109; CORE PROTEIN; LAMIVUDINE; UNCLASSIFIED DRUG;

DRUG PRESCRIBING; HEPATITIS;

ANTIVIRAL ACTIVITY; ARTICLE; BINDING SITE; CROSS LINKING; DNA REPLICATION; DRUG STRUCTURE; HEPATITIS B VIRUS; LIVER DISEASE; PRIORITY JOURNAL; VIRUS ASSEMBLY; VIRUS CORE; VIRUS GENOME; VIRUS INHIBITION; VIRUS NUCLEOCAPSID; VIRUS REPLICATION;

ACETYLCYSTEINE; AMINO ACID SUBSTITUTION; ANTIVIRAL AGENTS; BINDING SITES; CAPSID; DNA REPLICATION; DNA, VIRAL; HALF-LIFE; HEPATITIS B VIRUS; HEPATITIS B VIRUS, DUCK; HUMANS; MUTATION; NUCLEOCAPSID; PYRIDINES; PYRIMIDINES; RECOMBINANT PROTEINS; STEREOISOMERISM; TRIAZOLES; TUMOR CELLS, CULTURED; VIRAL CORE PROTEINS; VIRUS ASSEMBLY; VIRUS REPLICATION;

HEPATITIS B VIRUS;

EID: 0347928672     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1077215     Document Type: Article

References (12)

1. K. Deres, H. Rübsamen-Waigmann, Infection 27 (Suppl. 2), 545 (1999).
2. K. P. Fischer, K. S. Gutfreund, D. L. Tyrrell, Drug Resist. Updates 4, 118 (2001).
3. W. E. Delaney IV, S. Locarnini, T. Shaw, Antivir. Chem. Chemother. 12, 1 (2001).
4. O. Weber et al., Antiviral Res. 54, 69 (2002).
5. R. W. King et al., Antimicrob. Agents Chemother. 42, 3179 (1998).
6. M. Nassal, Curr. Top. Microbiol. Immunol. 214, 297 (1996).
7. M. A. Sells, M. L. Chen, G. Acs, Proc. Natl. Acad. Sci. U.S.A. 84, 1005 (1987).
8. M. Nassal, J. Virol. 66, 4107 (1992).
9. C. Lamberts, M. Nassal, I. Velhagen, H. Zentgraf, C. H. Schröder, J. Virol. 67, 3756 (1993).
10. M. L. Doyle, Curr. Opin. Biotechnol. 8, 31 (1997).
11. Materials and methods are available as supporting material on Science Online.
12. We thank M, Kraft, S. Kellermann, H. Küper, I. Leckebusch, K. Riedel, and M. Korkowski for technical support and D. Cramer for the artwork. We also thank H.-J. Schlicht (University of Ulm, Germany) for providing rabbit anti-HBV core polyclonal antisera and DHBV core protein and M. Nassal (University of Freiburg, Germany) for providing the plasmids pCH-9/3091, pCSla-C1, and pPLc-EHII. We thank G. Hewlett for critically reading the manuscript. This publication is dedicated to the late Ulrich Niewöhner. We miss his scientific excellence, inspiring mind, and warm personality.