Desalting of in-gel-digested protein sample with mini-C18 columns for matrix-assisted laser desorption ionization time of flight peptide mass fingerprinting

Analytical Biochemistry

Volumn 284, Issue 2, 2000, Pages 432-435

  Bagshaw, Rick D ^a,b   Callahan, John W ^b   Mahuran, Don J ^a,b  

^a UNIVERSITY OF TORONTO   (Canada)

^b HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

MASS SPECTROMETRY;

IN-GEL DIGESTED PROTEINS; MATRIX-ASSISTED-LASER DESORPTION IONIZATION-TIME-OF-FLIGHT; PEPTIDE MASS FINGERPRINTING;

DESALINATION;

GLYCOGEN PHOSPHORYLASE B; M PROTEIN;

ARTICLE; DESORPTION; EXTRACTION; GEL; IONIZATION; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; SAMPLING; SIGNAL NOISE RATIO;

EID: 0034633294     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4718     Document Type: Article

References (5)

1. Peptide mass maps: A highly informative approach to protein identification
2. Identification of in-gel digested proteins by complementary peptide mass fingerprinting and tandem mass spectrometry data obtained on an electrospray ionization quadrupole time-of-flight mass spectrometer
3. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels
4. Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: A method for the removal of silver ions to enhance sensitivity
5. Proteome analysis of polyacrylamide gel-separated proteins visualized by reversible negative staining using imidazole-zinc salts