Assay method for mitochondrial sterol 27-hydroxylase with 7α-hydroxy-4-cholesten-3-one as a substrate in the rat liver

Journal of Lipid Research

Volumn 44, Issue 12, 2003, Pages 2400-2405

  Ota, Yoshikazu ^a   Eto, Tada Aki ^a   Tanaka, Shun Ichi ^a   Sueta, Hideto ^a   Shiotsuki, Hironori ^a   Maeda, Yorio ^a   Une, Mizuho ^b   Chijiiwa, Kazuo ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

^b HIROSHIMA UNIVERSITY   (Japan)

Author keywords

5 cholestane 3 ,7 ,12 triol; 7 ,27 dihydroxy 4 cholesten 3 one; Adrenodoxin; Cholesterol; CYP27A1; High performance liquid chromatography; N octyl D glucopyranoside; NADPH adrenodoxin reductase; Solubilization

Indexed keywords

7ALPHA HYDROXYCHOLEST 4 EN 3 ONE; 7ALPHA,27 DIHYDROXY 4 CHOLESTEN 3 ONE; BILE ACID; DEUTERIUM; OCTYL GLUCOSIDE; STEROL; STEROL 27 HYDROXYLASE; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME ASSAY; FEMALE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTOPE LABELING; LIVER MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; CHOLESTENONES; CYTOCHROME P-450 CYP27A1; ELECTRON TRANSPORT; FEMALE; HYDROXYLATION; MITOCHONDRIA, LIVER; RATS; RATS, SPRAGUE-DAWLEY; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; STEROID HYDROXYLASES; SUBSTRATE SPECIFICITY; TEMPERATURE; TIME FACTORS;

EID: 0347093585     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: 10.1194/jlr.D200045-JLR200     Document Type: Article

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