Newly established assay method for 25-hydroxyvitamin D3 24-hydroxylase revealed much lower Km for 25-hydroxyvitamin D3 than for 1α,25-dihydroxyvitamin D3

Journal of Bone and Mineral Research

Volumn 16, Issue 1, 2001, Pages 57-62

  Taniguchi, T ^a   Eto, T A ^a   Shiotsuki, H ^a   Sueta, H ^a   Higashi, S ^a   Iwamura, T ^a   Okuda, K I ^a   Setoguchi, T ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

1 ,24,25 trihydroxyvitamin D3; 24R,25 dihydroxyvitamin D3; 25 hydroxyvitamin D3 24 hydroxylase; Adrenodoxin; NADPH adrenodoxin reductase

Indexed keywords

ADRENODOXIN; CALCIFEDIOL; CALCITRIOL; FERREDOXIN NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE REDUCTASE; OXYGENASE; POLYSORBATE 20; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; ENZYME ACTIVITY; ENZYME SUBSTRATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MICHAELIS CONSTANT; MITOCHONDRION; NONHUMAN; RAT; ULTRACENTRIFUGE; VITAMIN BLOOD LEVEL;

EID: 0035172727     PISSN: 08840431     EISSN: None     Source Type: Journal    
DOI: 10.1359/jbmr.2001.16.1.57     Document Type: Article

References (18)

1. Functional metabolism and molecular biology of vitamin D action
2. Recent progress in enzymology and molecular biology of enzymes involved in vitamin D metabolism
3. 3 promotes bone formation without causing excessive resorption in hypophosphatemic mice
4. 3 metabolite for both normal bone integrity and healing of tibial fracture in chicks
5. 3-responsive phenotype characteristic of growth zone chondrocytes
6. Vitamin D: Two dihydroxylated metabolites are required for normal chicken egg hatchability
7. 3 to calcitroic acid. Evidence for a pathway in kidney and bone involving 24-oxidation
8. 3 in rat kidney but not in intestine
9. Kinetic properties of 25-hydroxyvitamin D- and 1,25-dihydroxyvitamin D-24-hydroxylase from chick kidney
10. 3 1α-hydroxylase in rat kidney mitochondria
11. 3-1-hydroxylase. Inhibition in vitro by rat and pig tissues
12. 3 24R- and 1α-hydroxylase
13. Improved purification of bovine adrenal iron-sulfur protein
14. The purification and properties of NADPH-adrenodoxin reductase from bovine adrenocortical mitochondria
15. Statistical estimation in enzyme kinetics
16. Protein measurement with the Folin phenol reagent
17. 3 24-hydroxylase
18. 3-24-hydroxylase. Subcellular location and properties