Cloning, purification, and characterization of thermostable hypoxanthine-guanine phosphoribosyltransferase from Thermoanaerobacter tengcongensis

Protein Expression and Purification

Volumn 32, Issue 2, 2003, Pages 239-245

  Chen, Qiang ^a   You, Delin ^a   Hu, Meihao ^a   Gu, Xiaocheng ^a   Luo, Ming ^a,b   Lu, Shanyun ^a  

^a PEKING UNIVERSITY   (China)

^b University of Alabama at Birmingham   (United States)

Author keywords

Enzyme kinetics; Hypoxanthine guanine phosphoribosyltransferase; Thermoanaerobacter tengcongensis; Thermostable

Indexed keywords

ESCHERICHIA COLI; THERMOANAEROBACTER; THERMOANAEROBACTER TENGCONGENSIS;

EID: 0346936003     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2003.08.001     Document Type: Article

References (28)

1. R.L. Berens, E.C. Krug, J.J. Marr, in: J.J. Marr, M. Muller (Eds.), Biochemistry of Parasitic Organisms and Its Molecular Foundations, Academic Press, New York, 1995, pp. 89-117.
2. P.I. Patel, C.T. Caskey, HPRT and the Lesch-Nyhan syndrome, Bioassays 2 (1985) 4-8.
3. H.V. Hershey, M.W. Taylor, Nucleotide sequence and deduced amino acid sequence of Escherichia coli adenine phosphoribosyltransferase and comparison with other analogous enzymes, Gene 43 (1986) 287-293.
4. B. Hove-Jensen, K.W. Harlow, C.J. King, R.L. Switzer, Phosphoribosylpyrophosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene, J. Biol. Chem. 261 (1986) 6765-6771.
5. N. Hendrickson, T. Allen, B. Ullman, Molecular characterization of phosphoribosylpyrophosphate synthetase from Leishmania donovani, Mol. Biochem. Parasitol. 59 (1993) 15-27.
6. Q. Bao, Y. Tian, W. Li, Z. Xu, Z. Xuan, S. Hu, W. Dong, J. Yang, Y. Chen, Y. Xue, Y. Xu, X. Lai, L. Huang, X. Dong, Y. Ma, L. Ling, H. Tan, R. Chen, J. Wang, J. Yu, H. Yang, A complete sequence of T. tengcongensis genome, Genome Res. 12 (2002) 689-700.
7. Y. Xue, Y. Xu, Y. Liu, Y. Ma, P. Zhou, Thermoanaerobacter tengcongensis sp. nov., a novel anaerobic, saccharolytic, thermophilic bacterium isolated from a hot spring in Tengcong, China, Int. J. Syst. Evol. Microbiol. 51 (2001) 1335-1341.
8. S.F. Altschul, T.L. Madden, A.A. Schäffer, J. Zhang, Z. Zhang, W. Miller, D.J. Lipman, Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25 (1997) 3389-3402.
9. A.J. Walhout, G.F. Temple, M.A. Brasch, J.L. Hartley, M.A. Lorson, S. van den Heuvel, M. Vidal, GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes, Methods Enzymol. 328 (2000) 575-592.
10. F.W. Studier, A.H. Rosenberg, J.J. Dunn, J.W. Dubendorff, Use of T7 RNA polymerase to direct expression of cloned genes, Methods Enzymol. 185 (1990) 60-89.
11. A. Bairoch, R. Apweiler, The SWISS-PROT protein sequence data bank and its supplement TrEMBL, Nucleic Acids Res. 25 (1997) 31-36.
12. Y. Xu, J. Eads, J.C. Sacchettini, C. Grubmeyer, Kinetic mechanism of human hypoxanthine-guanine phosphoribosyltransferase: rapid phosphoribosyl transfer chemistry, Biochemistry 36 (1997) 3700-3712.
13. S.S. Deo, W.C. Tseng, R. Saini, S.C. Coles, R.S. Athwal, Purification and characterization of Escherichia coli xanthine-guanine phosphoribosyltransferase produced by plasmid pSV2gpt, Biochim. Biophys. Acta 839 (1985) 233-239.
14. D.T. Keough, A.L. Ng, B.T. Emmerson, J. de Jersey, Expression and properties of recombinant P. falciparum hypoxanthine-guanine phosphoribosyltransferase, Adv. Exp. Med. Biol. 431 (1998) 735-739.
15. H.F. Dovey, J.H. McKerrow, S.M. Aldritt, C.C. Wang, Purification and characterization of hypoxanthine-guanine phosphoribosyltransferase from Schistosoma mansoni. A potential target for chemotherapy, J. Biol. Chem. 261 (1986) 944-948.
16. T.E. Allen, B. Ullman, Molecular characterization and overexpression of the hypoxanthine-guanine phosphoribosyltransferase gene from Trypanosoma cruzi, Mol. Biochem. Parasitol. 65 (1994) 233-245.
17. L. Andreas, M. Olga, S. Halina, W. Matthias, K. Kasper, Purification, characterization and crystallization of thermostable anthranilate phosphoribosyltransferase from Sulfolobus solfataricus, Eur. J. Biochem. 268 (2001) 2246-2252.
18. L. Linde, K.F. Jensen, Uracil phosphoribosyltransferase from the extreme thermoacidophilic archaebacterium Sulfolobus shibatae is an allosteric enzyme, activated by GTP and inhibited by CTP, Biochim. Biophys. Acta 1296 (1996) 16-22.
19. H.K. Jensen, N. Mikkelsen, J. Neuhard, Recombinant uracil phosphoribosyltransferase from the thermophile Bacillus caldolyticus: expression, purification, and partial characterization, Protein Expr. Purif. 10 (1997) 356-364.
20. I.N. Sujay Subbayya, S. Sukumaran, K. Shivashankar, H. Balaram, Unusual substrate specificity of a chimeric hypoxanthine-guanine phosphoribosyltransferase containing segments from the Plasmodium falciparum and human enzymes, Biochem. Biophys. Res. Commun. 272 (2000) 596-602.
21. M.L. Free, R.B. Gordon, D.T. Keough, I.R. Beacham, B.T. Emmerson, J. de Jersey, Expression of active human hypoxanthine-guanine phosphoribosyltransferase in Escherichia coli and characterisation of the recombinant enzyme, Biochim. Biophys. Acta 1087 (1990) 205-211.
22. Y. Xu, J. Eads, J.C. Sacchettini, C. Grubmeyer, Kenetic mechanism of human hypoxanthine-guanine phosphoribosyltransferase: rapid phosphoribosyl transfer chemistry, Biochemistry 36 (1997) 3700-3712.
23. D.T. Keough, A.L. Ng, D.J. Winzor, B.T. Emmerson, J. de Jersey, Purification and characterization of Plasmodium falciparum hypoxanthine-guanine- xanthine phosphoribosyltransferase and comparison with the human enzyme, Mol. Biochem. Parasitol. 98 (1999) 29-41.
24. I.N. Sujay Subbayya, H. Balaram, Evidence for multiple active states of Plasmodium falciparum hypoxanthine-guanine-xanthine phosphoribosyltransferase, Biochem. Biophys. Res. Commun. 279 (2000) 433-437.
25. L. Yuan, S.P. Craig, J.H. McKerrow, C.C. Wang, The hypoxanthine-guanine phosphoribosyltransferase of Schistosoma mansoni. Further characterization and gene expression in Escherichia coli, J. Biol. Chem. 265 (1990) 13528-13532.
26. N.R. Munagala, M.S. Chin, C.C. Wang, Steady-state kinetics of the hypoxanthine-guanine-xanthine phosphoribosyltransferase from Tritrichomonasfoetus: the role of threonine-47, Biochemistry 37 (1998) 4045-4051.
27. A. Jardim, B. Ullman, The conserved serine-tyrosine dipeptide in Leishmania donovani hypoxanthine-guanine phosphoribosyltrans-ferase is essential for catalytic activity, J. Biol. Chem. 272 (1997) 8967-8973.
28. P.S. Monzani, J.D. Alfonzo, L. Simpson, G. Oliva, O.K. Thiemann, Cloning, characterization and preliminary crystallographic analysis of Leishmania hypoxanthine-guanine phosphoribosyltransferase, Biochim. Biophys. Acta 1598 (2002) 3-9.