Purification and characterization of Plasmodium falciparum hypoxanthine-guanine-xanthine phosphoribosyltransferase and comparison with the human enzyme

Molecular and Biochemical Parasitology

Volumn 98, Issue 1, 1999, Pages 29-41

  Keough, Dianne T ^a   Ng, Ai Lin ^a   Winzor, Donald J ^a   Emmerson, Bryan T ^b   De Jersey, John ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b PRINCESS ALEXANDRA HOSPITAL   (Australia)

Author keywords

Malaria; Phosphoribosyltransferase; Plasmodium falciparum; Purine salvage; Recombinant enzymes

Indexed keywords

HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; RECOMBINANT ENZYME;

ANIMAL EXPERIMENT; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME PURIFICATION; MALARIA; MASS SPECTROMETRY; NONHUMAN; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; ULTRACENTRIFUGATION;

ANIMALS; ENZYME ACTIVATION; ENZYME STABILITY; HUMANS; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; MASS SPECTROMETRY; MERCURIC CHLORIDE; MOLECULAR WEIGHT; PENTOSYLTRANSFERASES; PLASMODIUM FALCIPARUM; POTASSIUM CHLORIDE; RECOMBINANT PROTEINS; SPECIES SPECIFICITY; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; PLASMODIUM FALCIPARUM;

EID: 0032905985     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(98)00139-X     Document Type: Article

References (28)

1. Butler D., Maurice J., O'Brien C. Time to put malaria control on the global agenda. Nature. 386:1997;535-536.
2. Wang C.C. Parasite enzymes as potential targets for antiparasitic chemotherapy. J. Med. Chem. 27:1984;1-9.
3. Reyes P., Rathod P.K., Sanchez D.J., Mrema J.E.K., Rieckmann K.H., Heidrich H.-G. Enzymes of purine and pyrimidine metabolism from the human malarial parasite, Plasmodium falciparum. Mol. Biochem. Parasitol. 5:1982;275-290.
4. Queen S.A., van der Jagt D., Reyes P. Properties and substrate specificity of a purine phosphoribosyltransferase from the human malaria parasite, Plasmodium falciparum. Mol. Biochem. Parasitol. 30:1988;123-134.
5. Vasanthakumar G., Davis R.L., Sullivan M.A., Donahue J.P. Cloning and expression in Escherichia coli of a hypoxanthine-guanine phosphoribosyltransferase-encoding cDNA from Plasmodium falciparum. Gene. 1:1990;63-69.
6. Shahabuddin M., Scaife J. The gene for hypoxanthine phosphoribosyltransferase of Plasmodium falciparum complements a bacterial HPT mutation. Mol. Biochem. Parasitol. 41:1990;281-288.
7. Free M.L., Gordon R.B., Keough D.T., Beacham I.R., Emmerson B.T., de Jersey J. Expression of active human hypoxanthine-guanine phosphoribosyltransferase in Escherichia coli and characterisation of the recombinant enzyme. Biochim. Biophys. Acta. 1087:1990;205-211.
8. Zappia V., Oliva A., Cacciapuoti G., Galletti P., Mignucci G., Carteni-Farina M. Substrate specificity of 5′-methylthioadenosine phosphorylase from human prostate. Biochem. J. 175:1978;1043-1050.
9. Hughes S.H., Wahl G.M., Capecchi M.R. Purification and characterization of mouse hypoxanthine-guanine phosphoribosyltransferase. J. Biol. Chem. 250:1975;120-126.
10. Horton R.M., Hunt H.D., Ho S.N., Pullen J.K., Pease L.R. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene. 77:1989;61-68.
11. Keough D.T., Gee C.L., Emmerson B.T., de Jersey J. Role of cysteine and lysine residues in human hypoxanthine-guanine phosphoribosyltransferase. Griesmacher A., Chiba P., Muller M.M. Purine and Pyrimidine Metabolism in Man IX. 1997;331-335 Plenum, New York.
12. Keough D.T., Emmerson B.T., de Jersey J. Localization of the 5-phospho-α-D-ribosyl-1-pyrophosphate binding site of human hypoxanthine-guanine phosphoribosyltransferase. Biochim. Biophys. Acta. 1096:1991;95-100.
13. Jochimsen B., Nygaard P., Vestergaard T. Location on the chromosome of Escherichia coli of genes governing purine metabolism; adenosine deaminase (ADD); guanosine kinase (GSK); and hypoxanthine phosphoribosyltransferase (HPT). Mol. Gen. Genet. 143:1975;85-91.
14. Keough D.T., McConachie L.A., Gordon R.B., de Jersey J., Emmerson B.T. Human hypoxanthine-guanine phosphoribosyltransferase; development of a spectrophotometric assay and its use with detection and characterisation of mutant forms. Clin. Chim. Acta. 163:1987;301-308.
15. Gill S.C., von Hippel P.H. Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182:1989;319-326.
16. Xu Y., Eads J., Sacchettini J.C., Grubmeyer C. Kinetic mechanism of human hypoxanthine-guanine phosphoribosyltransferase: rapid phosphoribosyl transfer chemistry. Biochemistry. 36:1997;3700-3712.
17. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680.
18. Tuttle J.V., Krenitsky T.A. Purine phosphoribosyltransferases from Leishmania donovani. J. Biol. Chem. 255:1980;909-916.
19. Duggleby R.G. Regression analysis of nonlinear Arrhenius plots: an empirical model and computer program. Comput. Biol. Med. 14:1984;447-455.
20. Wynants J., Van Belle H. Single-run high-performance liquid chromatography of nucleotides, nucleosides and major purine bases and its application to different tissue extracts. Anal. Biochem. 144:1985;258-266.
21. Cohn E.J., Edsall J.T. Proteins, Amino Acids and Peptides as Dipolar Ions, New York: Reinhold, 1943.
22. King A., Melton D.W. Characterisation of cDNA clones for hypoxanthine-guanine phosphoribosyltransferase from the human malarial parasite, Plasmodium falciparum: comparisons to the mammalian gene and protein. Nucleic Acids Res. 15:1987;10469-10481.
23. Schimandle C.M., Mole L.A., Sherman I.W. Purification of hypoxanthine-guanine phosphoribosyltransferase of Plasmodium lophurae. Mol. Biochem. Parasitol. 23:1987;39-45.
24. Beck J.T., Wang C.C. The hypoxanthine-guanine-xanthine phosphoribosyltransferase from Tritrichomonas foetus has unique properties. Mol. Biochem. Parasitol. 60:1993;187-194.
25. Yuan L., Craig S.P., McKerrow J.H., Wang C.C. The hypoxanthine-guanine phosphoribosyltransferase of Schistosoma mansoni. J. Biol. Chem. 265:1990;13528-13532.
26. Ullman B. Pyrazolopyrimidine metabolism in parasitic protozoa. Pharm. Res. 1:1984;194-203.
27. Marr J.J. Purine analogs as chemotherapeutic agents in leishmaniasis and American trypanosomiasis. J. Lab. Clin. Med. 118:1991;111-119.
28. Allen T.E., Ullman B. Cloning and expression of the hypoxanthine-guanine phosphoribosyltransferase gene from Trypanosoma brucei. Nucleic Acids Res. 21:1993;5431-5438.