Arg-14 Loop of Site 3 Anemone Toxins: Effects of Glycine Replacement on Toxin Affinity

Biochemistry

Volumn 42, Issue 49, 2003, Pages 14515-14521

  Seibert, Anna L ^a   Liu, Jinrong ^b   Hanck, Dorothy A ^b   Blumenthal, Kenneth M ^a  

^a UNIVERSITY AT BUFFALO   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CARDIOLOGY; CHEMICAL BONDS; NEUROLOGY; SODIUM; TOXIC MATERIALS;

MUTANTS;

BIOCHEMISTRY;

ALANINE; ANTHOPLEURINE B; ARGININE; GLYCINE; ISOPROTEIN; LEUCINE; METHYL GROUP; NEUROTOXIN; SEA ANEMONE TOXIN; SODIUM CHANNEL; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CHANNEL GATING; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; GENE MUTATION; HUMAN; HUMAN CELL; MOLECULAR INTERACTION; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; SEA ANEMONE; STEREOSPECIFICITY; STRUCTURE ANALYSIS; TOXIN ANALYSIS; TOXIN STRUCTURE; WILD TYPE;

ALANINE; AMINO ACID SUBSTITUTION; ANIMALS; ARGININE; BINDING SITES; CELL LINE; CELL LINE, TUMOR; CNIDARIAN VENOMS; GLYCINE; HUMANS; KINETICS; MICE; MODELS, MOLECULAR; MUSCLE PROTEINS; MUTAGENESIS, SITE-DIRECTED; NERVE TISSUE PROTEINS; PATCH-CLAMP TECHNIQUES; PEPTIDES; PROTEIN STRUCTURE, TERTIARY; SODIUM CHANNELS;

ACTINIARIA; ANEMONE; ANIMALIA; INVERTEBRATA;

EID: 0346850582     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi035291d     Document Type: Article

References (28)

1. Blumenthal, K. M., and Seibert, A. L. (2003) Voltage-gated sodium channel toxins: poisons, probes and future promise, Cell. Biochem. Biophys. 32, 215-238.
2. Cestele, S., and Catterall, W. A. (2000) Molecular mechanisms of neurotoxin action on voltage-gated sodium channels, Biochimie 82, 883-92.
3. Catterall, W. A. (1992) Cellular and molecular biology of voltage-gated sodium channels, Physiol. Rev. 72, S15-S47.
4. Catterall, W. A. (1988) Structure and function of voltage-sensitive ion channels, Science 242, 50-61.
5. Catterall, W. A., and Beress, L. (1978) Sea anemone toxin and scorpion toxin share a common receptor site associated with the action potential sodium ionophore, J. Biol. Chem. 253, 7393-7396.
6. Hanck, D. A., and Sheets, M. F. (1995) Modification of inactivation in cardiac sodium channels: Ionic current studies with Anthopleurin-A toxin, J. Gen. Physiol. 106, 601-616.
7. Sheets, M. F., and Hanck, D. A. (1999) Gating of skeletal and cardiac muscle sodium channels in mammalian cells, J. Physiol. 514, 425-436.
8. Norton, R. S. (1991) Structure and structure-function relationships of sea anemone proteins that interact with the sodium channel, Toxicon 29. 1051-1084.
9. Landon, C., Sodano, P., Cornet, B., Bonmatin, J. M., Kopeyan, C., Rochat, H., Vovelle, F., and Ptak, M. (1997) Refined solution structure of the anti-mammal and anti-insect LqqIII scorpion toxin: comparison with other scorpion toxins. Proteins 28, 360-374.
10. Monks, S. A., Pallaghy, P. K., Scanlon, M. J., and Norton, R. S. (1995) Solution structure of the cardiostimulant polypeptide Anthopleurin-B and comparison with Anthopleurin-A, Structure 3, 791-803.
11. Pallaghy, P. K., Scanlon, M. J., Monks, S. A., and Norton, R. S. (1995) Three-dimensional structure in solution of the polypeptide cardiac stimulant Anthopleurin-A, Biochemistry 34, 3782-3794.
12. Fogh, R. H., Kem, W. R., and Norton, R. S. (1990) Solution structure of Neurotoxin I from the sea anemone Stichodacotyla helianthus, J. Biol. Chem. 265, 13016-13028.
13. Gould, A. R., Mabbutt, B. C., Llewellyn, L. E., Goss, N. H., and Norton, R. S. (1992) Linear and cyclic peptide analogues of the polypeptide cardiac stimulant, Anthopleurin-A: 1H-NMR and biological activity studies, Eur. J. Biochem. 206, 641-51.
14. Gallagher, M. J., and Blumenthal, K. M. (1992) Cloning and expression of wild-type and mutant forms of the cardiotonic polypeptide Anthopleurin B, J. Biol. Chem. 267, 13958-13963,
15. Gallagher, M. J., and Blumenthal, K. M. (1994) Importance of the unique cationic residues Arginine 12 and Lysine 49 in the activity of the cardiotonic polypeptide Anthopleurin B, J. Biol. Chem. 269, 254-259.
16. Dias-Kadambi, B. L., Combs, K. A., Drum, C. L., Hanck, D. A., and Blumenthal, K. M. (1996) Leucine 18, a hydrophobic residue essential for high affinity binding of Anthopleurin B to the voltage-sensitive sodium channel, J. Biol. Chem. 271, 23828-23835.
17. Khera, P. K., Benzinger, G. R., Lipkind, G., Drum, C. L., Hanck, D. A., and Blumenthal, K. M. (1995) Multiple cationic residues of Anthopleurin B that determine high affinity channel and isoform discrimination, Biochemistry 34, 8522-8541.
18. Benzinger, G. R., Kyle, J. W., Blumenthal, K. M., and Hanck, D. A. (1998) A specific interaction between the cardiac sodium channel and site-3 toxin Anthopleruin B, J. Biol. Chem. 273, 80-84.
19. Rogers, J. C., Qu, Y., Tanada, N., Scheuer, T., and Catterall, W. A. (1996) Molecular determinants of high affinity binding of α-scorpion toxin and sea anemone toxin in the S3-S4 extracellular loop in domain IV of the sodium channel α subunit. J. Biol. Chem. 271, 15950-15962.
20. Hirsch, J. K., and Quandt, F. N. (1996) Down-regulation of Na channel expression by A23 I87 in NIE-115 neuroblastoma cells, Brain Res. 706, 343-46.
21. Benzinger, G. R., Tonkovich, G. S., and Hanck, D. A. (1999) Augmentation of recovery from inactivation by site-3 Na channel toxins: A single-channel and whole-cell study of persistent currents, J. Gen. Physiol. 113, 333-346.
22. Kelso, G. J., Drum, C. L., Hanck, D. A., and Blumenthal, K. M. (1996) Role of Pro-13 in directing high-affinity binding of Anthopleurin B to the voltage-sensitive sodium channel, Biochemistry 25, 14157-14164.
23. Maggio, F., and King, G. F. (2002) Scanning mutagenesis of a Janus-faced atracotoxin reveals a bipartite surface patch that is essential for neurotoxic function, J. Biol. Chem. 277, 22806-13.
24. Sun, Y. M., Bosmans, F., Zhu, R. H., Goudet, C., Xiong, Y. M., Tytgat, J., and Wang, D. C. (2003) Importance of the conserved aromatic residues in the scorpion α-like toxin BmK MI, J. Biol. Chem. 278, 24125-24131.
25. Swartz, K., and MacKinnon. R. (1997) Hanatoxin modifies the gating of a voltage-dependent K+ channel through multiple binding sites, Neuron 18, 665-73.
26. Swartz, K., and MacKinnon, R. (1997) Mapping the Receptor Site for Hanatoxin, a Gating Modifier of Voltage-Dependent K+ Channels, Neuron 18, 675-82.
27. Jiang, Y., Ruta, V., Chen, J., Lee, A., and MacKinnon, R. (2003) The principle of gating charge movement in a voltage-dependent K+ channel, Nature 423, 42-48.
28. Gilles, N., Leipold, E., Chen, H., Heinemann, S. H., and Gordon, D. (2001) Effect of depolarization on binding kinetics of scorpion α-toxin highlights conformational changes of rat brain sodium channels, Biochemistry 40, 14576-84.