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Volumn 83, Issue 21, 2003, Pages 4441-4443

Optical trap setup for measuring microtubule pushing forces

Author keywords

[No Author keywords available]

Indexed keywords

ELECTRON TRAPS; HYDROLYSIS; MICROSCOPIC EXAMINATION; POLYMERIZATION; PROTEINS; REFRACTIVE INDEX; STIFFNESS;

EID: 0346846580     PISSN: 00036951     EISSN: None     Source Type: Journal    
DOI: 10.1063/1.1629796     Document Type: Article
Times cited : (33)

References (18)
  • 14
    • 0347329100 scopus 로고    scopus 로고
    • Ph.D. thesis, Leiden University, The Netherlands
    • M. E. Janson, Ph.D. thesis, Leiden University, The Netherlands, 2002.
    • (2002)
    • Janson, M.E.1
  • 15
    • 0347329101 scopus 로고    scopus 로고
    • note
    • 4 1064 nm laser (Spectra Physics). The trapped bead motion is detected with a He-Ne 633 nm laser and a quadrant photodiode for calibration purposes. We infer the stiffness from the roll-off frequency in the bead motion spectrum. The trap potential was measured by capturing beads in viscous glycerol, yielding a near-Gaussian profile with a width of app. 0.8 μm.
  • 16
    • 0346067858 scopus 로고    scopus 로고
    • note
    • x walls or silica porous beads (Asahi Glass Comp.).
  • 17
    • 0347329099 scopus 로고    scopus 로고
    • note
    • Parallel experiments (Janson, Ph.D. thesis) predict a catastrophe within ~30 s from the onset of stalling. The extended lifetime in this particular experiment is attributed to residual stabilizing agents in the growth solution.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.