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9
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0012589216
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note
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-6 N/m. Data acquisition and analysis is done using DAQ card (National Instruments PC-MIO-16XE-10) and LabVIEW.
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11
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0029752766
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12
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0012613207
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note
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Vesicle preparation: DDAB is mixed in ethanol at a concentration of 20 mM. 100 μ1 of this solution is spread on a 1 in.× 1 in. clean and dry teflon piece and incubated in the oven at a temperature of 50° over 8 h. 500 μ1 of PBS (2× buffer) at pH 7.4 is added to this so that the final lipid concentration is 4 mM. The solution is sonicated for 1 h to get vesicles with sizes ranging from 4 to 20 μm. The vesicles may be stored at 4 °C and used over two weeks. For fluorescence imaging of the membrane tubules Fluorescein Isothiocynate (excitation 497 nm and emission 521 nm) is mixed to PBS pH 7.4 at 2 nM concentration before sonication.
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14
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0030071248
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R. M. Hochmuth, J.-Y. Shao, J. Dai, and M. P. Sheetz, Biophys. J. 70, 358 (1996).
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15
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0012621125
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note
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9 C.
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