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Volumn 82, Issue 10, 2003, Pages 1631-1633

Nanomechanics of membrane tubulation and DNA assembly

Author keywords

[No Author keywords available]

Indexed keywords

BACKSCATTERING; ELASTIC MODULI; LASER BEAMS; OPTICAL SYSTEMS;

EID: 0037429925     PISSN: 00036951     EISSN: None     Source Type: Journal    
DOI: 10.1063/1.1559632     Document Type: Article
Times cited : (23)

References (16)
  • 9
    • 0012589216 scopus 로고    scopus 로고
    • note
    • -6 N/m. Data acquisition and analysis is done using DAQ card (National Instruments PC-MIO-16XE-10) and LabVIEW.
  • 12
    • 0012613207 scopus 로고    scopus 로고
    • note
    • Vesicle preparation: DDAB is mixed in ethanol at a concentration of 20 mM. 100 μ1 of this solution is spread on a 1 in.× 1 in. clean and dry teflon piece and incubated in the oven at a temperature of 50° over 8 h. 500 μ1 of PBS (2× buffer) at pH 7.4 is added to this so that the final lipid concentration is 4 mM. The solution is sonicated for 1 h to get vesicles with sizes ranging from 4 to 20 μm. The vesicles may be stored at 4 °C and used over two weeks. For fluorescence imaging of the membrane tubules Fluorescein Isothiocynate (excitation 497 nm and emission 521 nm) is mixed to PBS pH 7.4 at 2 nM concentration before sonication.
  • 15
    • 0012621125 scopus 로고    scopus 로고
    • note
    • 9 C.
  • 16
    • 0012581602 scopus 로고    scopus 로고
    • private communication
    • M. Rao and Y. Hatwalne (private communication).
    • Rao, M.1    Hatwalne, Y.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.