Effects of DNA Adsorption on the Phase Cycling of a Supported Mixed Phospholipid Bilayer

Nano Letters

Volumn 2, Issue 4, 2002, Pages 305-309

  Leonenko, Zoya ^a   Cramb, David ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0346743190     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl0156989     Document Type: Article

References (18)

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11. We employed MAC (magnetic A/C) mode, where a magnetically coated probe oscillates near its resonant frequency driven by an alternating magnetic field. For MAC mode, changes in height and phase shift (friction/viscoelasticity) are measured during the scan. This technique has proven to be advantageous for measuring supported planar bilayers in liquid media. All images were taken using a Pico SPM microscope with a AFMS-165 scanner (Molecular Imaging Inc., Phoenix, Az.). Au-Cr coated Maclevers (Molecular Imaging Inc., Phoenix, Az.) were used for MAC mode imaging. Their specifications include a length of 85 μm, a force constant of 0.06-0.1 N/m, and a resonant frequency of 20-40 kHz in liquid. The standard MAC mode fluid cell (Molecular Imaging, Phoenix, AZ) was used throughout. The scanning speed was 1-2 lines/s. The height scale was calibrated using colloidal gold spheres of well-defined size. For elevated temperature experiments the AFM Temperature Controller and Hot MacMode Stage from Molecular Imaging Inc., Phoenix, AZ, were used.
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