Purification, cDNA cloning, and secretory properties of FLRG protein from PC12 cells and the distribution of FLRG mRNA and protein in rat tissues

Archives of Histology and Cytology

Volumn 66, Issue 4, 2003, Pages 367-381

  Ohsawa, Yoshiyuki ^a   Zhang, Guiqin ^a   Kametaka, Satoshi ^a   Shibata, Masahiro ^a   Koike, Masato ^a   Waguri, Satoshi ^a   Uchiyama, Yasuo ^a  

^a OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVIN A; COMPLEMENTARY DNA; FOLLISTATIN; FOLLISTATIN RELATED PROTEIN; MESSENGER RNA; PROTEIN BCL 2; PROTEIN FLRG; UNCLASSIFIED DRUG;

ADOLESCENT; ADRENAL CORTEX; ADRENAL GLAND; ADRENAL MEDULLA; AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL STRAIN; CONTROLLED STUDY; DNA SEQUENCE; EMBRYO; FEMALE; FETUS; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; MALE; MOLECULAR CLONING; NONHUMAN; OVARY; POLYMERASE CHAIN REACTION; PROTEIN ISOLATION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN SECRETION; PROTEIN TRANSPORT; RAT; SECRETORY GRANULE; SPERMATOGENESIS; TESTIS; TISSUE DISTRIBUTION;

ACTIVINS; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOTTING, WESTERN; CLONING, MOLECULAR; DATABASES; DNA, COMPLEMENTARY; FEMALE; FOLLISTATIN-RELATED PROTEINS; GENE LIBRARY; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; INHIBIN-BETA SUBUNITS; MALE; MOLECULAR SEQUENCE DATA; PC12 CELLS; POLYMERASE CHAIN REACTION; RATS; RATS, WISTAR; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SEQUENCE HOMOLOGY, AMINO ACID; TIME FACTORS; TISSUE DISTRIBUTION; TRANSFECTION; TRANSFORMING GROWTH FACTOR BETA;

EID: 0346157219     PISSN: 09149465     EISSN: None     Source Type: Journal    
DOI: 10.1679/aohc.66.367     Document Type: Article

References (45)

1. Arai KY, Tsuchida K, Uehara K, Taya K, Sugino H: Characterization of rat follistatin-related gene: effects of estrous cycle stage and pregnancy on its messenger RNA expression in rat reproductive tissues. Biol Reprod 68: 199-206 (2003).
2. Bartholin L, Maguer-Satta V, Hayette S, Martel S, Gadoux M, Bertrand S, Corbo L, Lamadon C, Morera AM, Magaud JP, Rimokh R: FLRG, an activin-binding protein, is a new target of TGFβ transcription activation through Smad proteins. Oncogene 20: 5409-5419 (2001).
3. Bartholin L, Maguer-Satta V, Hayette S, Martel S, Gadoux M, Corbo L, Magaud JP, Rimokh R: Transcription activation of FLRG and follistatin by activin A, through Smad proteins, participates in a negative feedback loop to modulate activin A function. Oncogene 21: 2227-2235 (2002).
4. Batistatou A, Volonte C, Greene LA: Nerve growth factor employs multiple pathways to induce primary response genes in PC12 cells. Mol Biol Cell 3: 363-371 (1992).
5. Batistatou A, Greene LA: Internucleosomal DNA cleavage and neuronal cell survival/death. J Cell Biol 122: 523-532(1993).
6. Campanelli JT, Ferns M, Hoch W, Rupp F, von Zastrow M, Hall Z, Scheller RH: Agrin: a synaptic basal lamina protein that regulates development of the neuromuscular junction. Cold Spring Harb Symp Quant Biol 57: 461-472(1992).
7. Cataldo NA, Rabinovici J, Fujimoto VY, Jaffe RB: Follistatin antagonizes the effects of activin-A on steroido-genesis in human luteinizing granulosa cells. J Clin Endocrinol Metab 79: 272-277 (1994).
8. Chen C, Okayama H: High-efficiency transformation of mammalian cells by plasmid DNA. Mol Cell Biol 7: 2745-2752 (1987).
9. Cool DR, Normant E, Shen F, Chen HC, Pannell L, Zhang Y, Loh YP: Carboxypeptidase E is a regulated secretory pathway sorting receptor: genetic obliteration leads to endocrine disorders in Cpe (fat) mice. Cell 88: 73-83 (1997).
10. Corrigan AZ, Bilezikjian LM, Carroll RS, Bald LN, Schmelzer CH, Fendly BM, Mason AJ, Chin WW, Schwall RH, Vale W: Evidence for an autocrine role of activin B within rat anterior pituitary cultures. Endocrinology 128: 1682-1684 (1991).
11. Cosgaya JM, Perona R, Aranda A: Retinoic acid induces secretion of transforming growth factors by PC12 pheochromocytoma cells. Oncogene 14: 579-587 (1997).
12. Cowley DJ, Moore YR, Darling DS, Joyce PB, Gorr SU: N-and C-terminal domains direct cell type-specific sorting of chromogranin A to secretory granules. J Biol Chem 275: 7743-7748 (2000).
13. Gorr SU, Huang XF, Cowley DJ, Kuliawat R, Arvan P: Disruption of disulfide bonds exhibits differential effects on trafficking of regulated secretory proteins. Am J Physiol 277: C121-131 (1999).
14. Gorr SU, Jain RK, Kuehn U, Joyce PB, Cowley DJ: Comparative sorting of neuroendocrine secretory proteins: a search for common ground in a mosaic of sorting models and mechanisms. Mol Cell Endocrinol 172: 1-6 (2001).
15. Gumbiner B, Kelly RB: Two distinct intracellular pathways transport secretory and membrane glycoproteins to the surface of pituitary tumor cells. Cell 28: 51-59 (1982).
16. Hashimoto M, Shoda A, Inoue S, Yamada R, Kondo T, Sakurai T, Ueno N, Muramatsu M: Functional regulation of osteoblastic cells by the interaction of activin-A with follistatin. J Biol Chem 267: 4999-5004 (1992).
17. Hayette S, Gadoux M, Martel S, Bertrand S, Tigaud I, Magaud JP, Rimokh R: FLRG (follistatin-related gene), a new target of chromosomal rearrangement in malignant blood disorders. Oncogene 16: 2949-2954 (1998).
18. Hosaka M, Watanabe T, Sakai Y, Uchiyama Y, Takeuchi T: Identification of a chromogranin A domain that mediates binding to secretogranin III and targeting to secretory granules in pituitary cells and pancreatic beta-cells. Mol Biol Cell 13: 3388-3399 (2002).
19. Iwasaki S, Hattori A, Sato M, Tsujimoto M, Kohno M: Characterization of the bone morphogenetic protein-2 as a neurotrophic factor. Induction of neuronal differentiation of PC12 cells in the absence of mitogen-activated protein kinase activation. J Biol Chem 271: 17360-17365 (1996).
20. Kogawa K, Nakamura T, Sugino K, Takio K, Titani K, Sugino H: Activin-binding protein is present in pituitary. Endocrinology 128: 1434-1440 (1991).
21. Koike M, Shibata M, Ohsawa Y, Kametaka S, Waguri S, Kominami E, Uchiyama Y: The expression of tripeptidyl peptidase I in various tissues of rats and mice. Arch Histol Cytol 65: 219-232 (2002).
22. Lane TF, Sage EH: The biology of SPARC, a protein that modulates cell-matrix interactions. FASEB J 8: 163-173 (1994).
23. Liu J, Vanttinen T, Hyden-Granskog C, Voutilainen R: Regulation of follistatin-related gene (FLRG) expression by protein kinase C and prostaglandin E (2) in cultured granulosa-luteal cells. Mol Hum Reprod 8: 992-997 (2002).
24. Maguer-Satta V, Bartholin L, Jeanpierre S, Gadoux M, Bertrand S, Martel S, Magaud JP, Rimokh R: Expression of FLRG, a novel activin A ligand, is regulated by TGF-beta and during hematopoiesis [corrected]. Exp Hematol 29: 301-308 (2001).
25. Mason IJ, Taylor A, Williams JG, Sage H, Hogan BL: Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell 'culture shock' glycoprotein of Mr 43,000. EMBO J 5: 1465-1472 (1986).
26. Matzuk MM, Lu N, Vogel H, Sellheyer K, Roop DR, Bradley A: Multiple defects and perinatal death in mice deficient in follistatin. Nature 374: 360-363 (1995).
27. Mendis DB, Brown IR: Expression of the gene encoding the extracellular matrix glycoprotein SPARC in the developing and adult mouse brain. Brain Res Mol Brain Res 24: 11-19 (1994).
28. Mendis DB, Shahin S, Gurd JW, Brown IR: SC1, a SPARC-related glycoprotein, exhibits features of an ECM component in the developing and adult brain. Brain Res 713: 53-63 (1996).
29. Nakamura T, Takio K, Eto Y, Shibai H, Titani K, Sugino H: Activin-binding protein from rat ovary is follistatin. Science 247: 836-838 (1990).
30. Nitatori T, Sato N, Waguri S, Karasawa Y, Araki H, Shibanai K, Kominami E, Uchiyama Y: Delayed neuronal death in the CA1 pyramidal cell layer of the gerbil hippocampus following transient ischemia is apoptosis. J Neurosci 15: 1001-1011 (1995).
31. Patthy L, Nikolics K: Functions of agrin and agrin-related proteins. Trends Neurosci 16: 76-81 (1993).
32. Sage EH, Vernon RB: Regulation of angiogenesis by extracellular matrix: the growth and the glue. J Hypertens Suppl 12: S145-152 (1994).
33. Sato N, Hotta K, Waguri S, Nitatori T, Tohyama K, Tsujimoto Y, Uchiyama Y: Neuronal differentiation of PC12 cells as a result of prevention of cell death by bcl-2. J Neurobiol 25: 1227-1234 (1994).
34. Schneyer A, Tortoriello D, Sidis Y, Keutmann H, Matsuzaki T, Holmes W: Follistatin-related protein (FSRP): a new member of the follistatin gene family. Mol Cell Endocrinol 180: 33-38 (2001).
35. Shibanuma M, Mashimo J, Mita A, Kuroki T, Nose K: Cloning from a mouse osteoblastic cell line of a set of transforming-growth-factor-beta1 1-regulated genes, one of which seems to encode a follistatin-related polypeptide. Eur J Biochem 217: 13-19 (1993).
36. Sumitomo K, Kurisaki A, Yamakawa N, Tsuchida K, Shimizu E, Sone S, Sugino H: Expression of a TGF-beta1 inducible gene, TSC-36, causes growth inhibition in human lung cancer cell lines. Cancer Lett 155: 37-46 (2000).
37. Tooze S, Seethaler G, Shields D: The slippery slopes of secretion. Trends Cell Biol 8: 211-213 (1998).
38. Tooze SA, Martens GJ, Huttner WB: Secretory granule biogenesis: rafting to the SNARE. Trends Cell Biol 11: 116-122 (2001).
39. Tortoriello DV, Sidis Y, Holtzman DA, Holmes WE, Schneyer AL: Human follistatin-related protein: a structural homologue of follistatin with nuclear localization. Endocrinology 142: 3426-3434 (2001).
40. Tsim KW, Ruegg MA, Escher G, Kroger S, McMahan UJ: cDNA that encodes active agrin. Neuron 8: 677-689 (1992).
41. Tsuchida K, Arai KY, Kuramoto Y, Yamakawa N, Hasegawa Y, Sugino H: Identification and characterization of a novel follistatin-like protein as a binding protein for the TGF-beta family. J Biol Chem 275: 40788-40796 (2000).
42. Tuuri T, Eramaa M, Van Schaik RH, Ritvos O: Differential regulation of inhibin/activin alpha-and beta A-subunit and follistin mRNAs by cyclic AMP and phorbol ester in cultured human granulose-luteal cells. Mol Cell Endocrinol 121: 1-10 (1996).
43. Ying SY, Becker A, Swanson G, Tan P, Ling N, Esch F, Ueno N, Shimasaki S, Guillemin R: Follistatin specifically inhibits pituitary follicle stimulating hormone release in vitro. Biochem Biophys Res Commun 149: 133-139 (1987).
44. Zhang G, Ohsawa Y, Kametaka S, Shibata M, Waguri S, Uchiyama Y: Regulation of FLRG expression in rat primary astroglial cells and injured brain tissue by transforming growth factor-beta1 (TGF-beta1). J Neurosci Res 72: 33-45 (2003).
45. Zhao GQ, Chen YX, Liu XM, Xu Z, Qi X: Mutation in Bmp7 exacerbates the phenotype of Bmp8a mutants in spermatogenesis and epididymis. Dev Biol 240: 212-222 (2001).