Inhibition of pancreatic adenocarcinoma cellular invasiveness by blebbistatin: A novel myosin II inhibitor

Biochemical and Biophysical Research Communications

Volumn 313, Issue 4, 2004, Pages 992-997

  Duxbury, Mark S ^a   Ashley, Stanley W ^a   Whang, Edward E ^a  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

Adenocarcinoma; Adhesion; Blebbistatin; Cancer; Fibronectin; Invasion; Myosin II; Pancreatic

Indexed keywords

1 PHENYL 1,2,3,4 TETRAHYDRO 4 HYDROXYPYRROLO[2,3 B] 7 METHYLQUINOLIN 4 ONE; 2 PYRROLIDONE DERIVATIVE; BETA1 INTEGRIN; ENZYME INHIBITOR; FIBRONECTIN; FIBRONECTIN RECEPTOR; GELATINASE A; GELATINASE B; MYOSIN II INHIBITOR; QUINOLINE DERIVATIVE; RECEPTOR SUBUNIT; UNCLASSIFIED DRUG;

ARTICLE; CANCER CELL CULTURE; CANCER INVASION; CELL ADHESION; CELL INVASION; CELL MIGRATION; CELL PROLIFERATION; CELL SPREADING; CONCENTRATION RESPONSE; DRUG EFFECT; ENANTIOMER; ENZYME ACTIVITY; PANCREAS ADENOCARCINOMA; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0346098149     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2003.12.031     Document Type: Article

References (28)

1. Liotta L.A., Schiffmann E. Tumour motility factors. Cancer Surv. 7:1988;631-652.
2. Cooper J.A. The role of actin polymerization in cell motility. Annu. Rev. Physiol. 53:1991;585-605.
3. Corson M.A., Sellers J.R., Adelstein R.S., Schoenberg M. Substance P contracts bovine tracheal smooth muscle via activation of myosin light chain kinase. Am. J. Physiol. 259:1990;C258-C265.
4. Gerthoffer W.T. Regulation of the contractile element of airway smooth muscle. Am. J. Physiol. 261:1991;L15-L28.
5. Holzapfel G., Wehland J., Weber K. Calcium control of actin-myosin based contraction in triton models of mouse 3T3 fibroblasts is mediated by the myosin light chain kinase (MLCK)-calmodulin complex. Exp. Cell Res. 148:1983;117-126.
6. Ludowyke R.I., Peleg I., Beaven M.A., Adelstein R.S. Antigen-induced secretion of histamine and the phosphorylation of myosin by protein kinase C in rat basophilic leukemia cells. J. Biol. Chem. 264:1989;12492-12501.
7. Mahajan R.K., Vaughan K.T., Johns J.A., Pardee J.D. Actin filaments mediate dictyostelium myosin assembly in vitro. Proc. Natl. Acad. Sci. USA. 86:1989;6161-6165.
8. Cross R.A., Jackson A.P., Citi S., Kendrick-Jones J., Bagshaw C.R. Active site trapping of nucleotide by smooth and non-muscle myosins. J. Mol. Biol. 203:1988;173-181.
9. Ikebe M., Koretz J., Hartshorne D.J. Effects of phosphorylation of light chain residues threonine 18 and serine 19 on the properties and conformation of smooth muscle myosin. J. Biol. Chem. 263:1988;6432-6437.
10. Ikebe M., Hartshorne D.J. Phosphorylation of smooth muscle myosin at two distinct sites by myosin light chain kinase. J. Biol. Chem. 260:1985;10027- 10031.
11. Ikebe M., Hartshorne D.J., Elzinga M. Identification, phosphorylation, and dephosphorylation of a second site for myosin light chain kinase on the 20,000-dalton light chain of smooth muscle myosin. J. Biol. Chem. 261:1986;36-39.
12. Gillespie G.Y., Soroceanu L., Manning T.J. Jr., Gladson C.L., Rosenfeld S.S. Glioma migration can be blocked by nontoxic inhibitors of myosin II. Cancer Res. 59:1999;2076-2082.
13. Umemoto S., Bengur A.R., Sellers J.R. Effect of multiple phosphorylations of smooth muscle and cytoplasmic myosins on movement in an in vitro motility assay. J. Biol. Chem. 264:1989;1431-1436.
14. Somlyo A.P., Somlyo A.V. Signal transduction by G-proteins, rho-kinase and protein phosphatase to smooth muscle and non-muscle myosin II. J. Physiol. 522(Pt 2):2000;177-185.
15. Straight A.F., Cheung A., Limouze J., Chen I., Westwood N.J., Sellers J.R., Mitchison T.J. Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor. Science. 299:2003;1743-1747.
16. Kaneko K., Satoh K., Masamune A., Satoh A., Shimosegawa T. Myosin light chain kinase inhibitors can block invasion and adhesion of human pancreatic cancer cell lines. Pancreas. 24:2002;34-41.
17. Raitano A.B., Scuderi P., Korc M. Binding and biological effects of tumor necrosis factor and gamma interferon in human pancreatic carcinoma cells. Pancreas. 5:1990;267-277.
18. Akiyama S.K., Olden K., Yamada K.M. Fibronectin and integrins in invasion and metastasis. Cancer Metastasis Rev. 14:1995;173-189.
19. Arao S., Masumoto A., Otsuki M. Beta 1 integrins play an essential role in adhesion and invasion of pancreatic carcinoma cells. Pancreas. 20:2000;129-137.
20. Sellers J.R. Regulation of cytoplasmic and smooth muscle myosin. Curr. Opin. Cell Biol. 3:1991;98-104.
21. - channel activity in human cervical cancer cells. Pflugers Arch. 444:2002;276-285.
22. 2+ influx in endothelial cells. FASEB J. 12:1998;341-348.
23. + exchange: role of cell density and myosin light chain phosphorylation. Am. J. Physiol. 272:1997;C1968-C1979.
24. - cotransport in Ehrlich cells: regulation by protein phosphatases and kinases. Am. J. Physiol. 275:1998;C239-C250.
25. Makishima M., Honma Y., Hozumi M., Sampi K., Hattori M., Motoyoshi K. Induction of differentiation of human leukemia cells by inhibitors of myosin light chain kinase. FEBS Lett. 287:1991;175-177.
26. Makishima M., Honma Y., Hozumi M., Nagata N., Motoyoshi K. Differentiation of human monoblastic leukemia U937 cells induced by inhibitors of myosin light chain kinase and prevention of differentiation by granulocyte-macrophage colony-stimulating factor. Biochim. Biophys. Acta. 1176:1993;245-249.
27. Nagatsu T., Suzuki H., Kiuchi K., Saitoh M., Hidaka H. Effects of myosin light-chain kinase inhibitor on catecholamine secretion from rat pheochromocytoma PC12h cells. Biochem. Biophys. Res. Commun. 143:1987;1045-1048.
28. Pasternak C., Spudich J.A., Elson E.L. Capping of surface receptors and concomitant cortical tension are generated by conventional myosin. Nature. 341:1989;549-551.