Purification and characterization of a phytate-degrading enzyme from germinated oat (Avena sativa)

Journal of the Science of Food and Agriculture

Volumn 79, Issue 11, 1999, Pages 1453-1460

  Greiner, Ralf ^a   Larsson Alminger, Marie ^b  

^a FEDERAL RESEARCH INSTITUTE OF NUTRITION AND FOOD   (Germany)

^b CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

Author keywords

Myo inositol phosphate; Oat; Phosphatase; Phytase; Phytate

Indexed keywords

AVENA SATIVA; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; HYDROLYSIS; PHYTASE; PHYTATE;

EID: 0345493850     PISSN: 00225142     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0010(199908)79:11<1453::AID-JSFA386>3.0.CO;2-R     Document Type: Article

References (43)

1. Andersson H, Nävert B, Bingham SR, Englyst HN and Cummings JH, The effects of breads containing similar amounts of phytate but different amounts of wheat bran on calcium, zinc and iron balance in man. Br J Nutr 50:503-510 (1983).
2. Brune M, Rossander L, Hallberg L, Gleerup A and Sandberg A-S, Iron absorption from bread in humans: inhibiting effects of cereal fiber, phytate and inositol phosphates with different numbers of phosphate groups. J Nutr 122:442-449 (1992).
3. Morris ER, Phytate and mineral bioavailability, in Phytic acid: Chemistry and application, ed Graf E, Pilatus Press, Minneapolis. pp 57-76 (1986).
4. Nävert B, Sandström B and Cederblad A, Reduction of phytate content of bran by leavening in bread and its effect on zinc absorption in man. Br J Nutr 53:47-53 (1985).
5. Lönnerdal B, Sandberg A-S, Sandström B and Kunz C, Inhibitory effects of phytic acid and other inositol phosphates on zinc and calcium absorption in suckling rats. J Nutr 119:211-214 (1989).
6. Sandström B and Sandberg A-S, Inhibitory effects of isolated inositol phosphates on zinc absorption in humans. J Trace Elements Electrolytes Health Dis 6:99-103 (1992).
7. Sandberg A-S, Rossander L and Turk M, Dietary Aspergillus niger phytase increases iron absorption in humans. J Nutr 126:476-480 (1996).
8. Hallfrisch J and Behall KM, Evaluation of foods and physiological responses to menus in which fat content was lowered by replacement with oatrim. Cereal Foods World 42:100-103 (1997).
9. Larsson M and Sandberg A-S, Malting of oats in a pilot-plant process. Effects of heat treatment, storage and soaking conditions on phytate reduction. J Cereal Sci 21:87-95 (1995).
10. Bradford M, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein - dye binding. Anal Biochem 72:248-254 (1976).
11. Heinonen JK and Lahti RJ, A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatase. Anal Biochem 113:313-317 (1981).
12. Sandberg AS and Ahderinne R, HPLC method for determination of inositol tri-, tetra-, penta-, and hexaphosphates in foods and intestinal contents. J Food Sci 51:547-550 (1986).
13. Skoglund E, Carlsson N-G and Sandberg A-S, Determination of isomers of inositol mono-to hexaphosphates in selected foods and intestinal contents using high-performance ion chromatography. J Agric Food Chem 45:431-436 (1997).
14. Phillippy BQ and Bland JM, Gradient ion chromatography of inositol phosphates. Anal Biochem 175:162-166 (1988).
15. Reisfeld RA, Lewis UI and Williams DA, Disc electrophoresis of basic proteins in polyacrylamide gels. Nature 195:281-283 (1962).
16. Dorn G, Genetic analysis of the phosphatase in Aspergillus nidulans. Genet Res 6:13-26 (1965).
17. Laemmli UK, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685 (1970).
18. Bartnik M and Dulska J, Studies on the reduction of phytate content in oat, maize and buckwheat grain. Food Technol Nutr 17:33-38 (1987).
19. Gibson DM and Ullah AHJ, Purification and characterization of phytase from cotyledons of germinating soybean seeds. Arch Biochem Biophys 260:503-513 (1988).
20. Konietzny U, Greiner R and Jany K-D, Purification and characterization of a phytase from spelt. J Food Biochem 18:165-183 (1995).
21. Lolas GM and Markakis P, The phytase of navy beans (Phaseolus vulgaris). J Food Sci 42:1094-1106 (1977).
22. Laboure A-M, Gagnon J and Lescure A-M, Purification and characterization of a phytase (myo-inositolhexakisphosphate phosphohydrolase) accumulated in maize (Zea mays) seedlings during germination. Biochem J 295:413-419 (1993).
23. Silva LG and Trugo LC, Characterization of phytase activity in lupin seed. J Food Biochem 20:329-340 (1996).
24. Greiner R, Konietzny U and Jany K-D, Purification and characterization of two phytases from Escherichia coli. Arch Biochem Biophys 303:107-113 (1993).
25. Greiner R, Haller E, Konietzny U and Jany K-D, Purification and characterization of a phytase from Klebsiella terrigena. Arch Biochem Biophys 341:201-206 (1997).
26. Greiner R, Konietzny U and Jany K-D, Purification and properties of a phytase from rye. J Food Biochem 22:143-161 (1998).
27. Greiner R, Jany K-D and Larsson Alminger M, Identification and properties of phytate-degrading enzymes from barley (Hordeum vulgare). J Cereal Sci 30:11 (1999).
28. Hübel F and Beck E, Maize root phytase. Plant Physiol 112:1429-1436 (1996).
29. Irving GCJ, Phytase, in Inositol phosphates: Their chemistry, biochemistry and physiology, ed by Cosgrove DJ, Elsevier, Amsterdam, pp 85-98 (1980).
30. Nayini NR and Markakis P, Phytases, in Phytic acid: Chemistry and applications, ed Graf E, Pilatus Press, Minneapolis. pp 101-118 (1986).
31. Powar VK and Jagannathan V, Purification and properties of phytate-specific phosphatase from Bacillus subtilis. J Bacteriol 151:1102-1108 (1982).
32. Shah V and Parekh LJ, Phytase from Klebsiella Sp. No. PG-2: Purification and properties. Ind J Biochem Biophys 27:98-102 (1990).
33. Ullah, AHJ, Aspergillus ficuum phytase: Partial primary structure, substrate selectivity, and kinetic characterization. Prep Biochem 18:443-458 (1988).
34. Li M, Osaki M, Honma M and Tadano T, Purification and characterization of phytase induced in tomato roots under phosphorus-deficient conditions. Soil Sci Plant Nutr 43:179-190 (1997).
35. Wodzinski RJ and Ullah AHJ, Phytase. Adv Appl Microbiol 42:263-302 (1996).
36. Maiti IB, Majumber AL and Biswas BB, Purification and mode of action of phytase from Phaseolus aureus. Phytochemistry 13:1047-1051 (1974).
37. Nagai Y and Funahashi S, Phytase (myoinositolhexaphosphate phosphohydrolase) from wheat bran. Agric Biol Chem 26:794-803 (1962).
38. 2. Biochim Biophys Acta 302:326-328 (1973).
39. Eastwood D and Laidman DL, The mobilization of macronutrient elements in the germinating wheat grain. Phytochemistry 10:1275-1284 (1971).
40. Larsson M and Sandberg A-S, Phytate reduction in oats during malting. J Food Sci 57:994-997 (1992).
41. McCance RA and Widdowson EM, Activity of the phytase in different cereals and its resistance to dry heat. Nature 153:650 (1944).
42. Zyla K, Moulds phytases and their application in the food industry. World J Microbiol Biotechnol 8:467-472 (1992).
43. Jones BL and Marinac LA, Purification, identification, and partial characterization of a barley protein that inhibits green malt endoproteinases. J Am Soc Brew Chem 55:58-64 (1997).