Simple and efficient method for isolation and cultivation of endoscopically obtained human colonocytes

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 285, Issue 6 48-6, 2003, Pages

  Seidelin, Jakob B ^a   Horn, Thomas ^a   Nielsen, Ole H ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Apoptosis; Colonocytes; Primary culture; Viability

Indexed keywords

BROMINE DERIVATIVE; BUFFER; COLLAGEN; COLLAGEN GEL; INSULIN; OXYGEN; SELENIUM; TRANSFERRIN;

ARTICLE; CELL ASSAY; CELL CULTURE; CELL ISOLATION; CELL VIABILITY; CHELATION; CLINICAL ARTICLE; COLON BIOPSY; COLON MUCOSA; COLONOSCOPY; CONTROLLED STUDY; CRYPT CELL; CULTURE MEDIUM; EPITHELIUM CELL; FLOW CYTOMETRY; GEL; HUMAN; HUMAN CELL; HUMAN TISSUE; INCUBATION TIME; IRRITABLE COLON; MATERIAL COATING; PHASE CONTRAST MICROSCOPY; POROSITY; PRIORITY JOURNAL; TECHNIQUE; TEMPERATURE DEPENDENCE; TRANSMISSION ELECTRON MICROSCOPY; VALIDATION PROCESS;

EID: 0345357258     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.00533.2002     Document Type: Article

References (25)

1. Baten A, Sakamoto K, and Shamsuddin AM. Long-term culture of normal human colonic epithelial cells in vitro. FASEB J 6: 2726-2734, 1992.
2. Batlle E, Henderson JT, Beghtel H, van den Born MM, Sancho E, Huls G, Meeldijk J, Robertson J, van de Wetering M, Pawson T, and Clevers H. β-Catenin and TCF mediate cell positioning in the intestinal epithelium by controlling the expression of EphB/ephrinB. Cell 111: 251-263, 2002.
3. Beck PL and Podolsky DK. Growth factors in inflammatory bowel disease. Inflamm Bowel Dis 5: 44-60, 1999.
4. Booth C, O'Shea JA, and Potten CS. Maintenance of functional stem cells in isolated and cultured adult intestinal epithelium. Exp Cell Res 249: 359-366, 1999.
5. Booth C, Patel S, Bennion GR, and Potten CS. The isolation and culture of adult mouse colonic epithelium. Epithelial Cell Biol 4: 76-86, 1995.
6. Deveney CW, Rand-Luby L, Rutten MJ, Luttropp CA, Fowler WM, Land J, Meichsner CL, Farahmand M, Sheppard BC, Crass RA, and Deveney KE. Establishment of human colonic epithelial cells in long-term culture. J Surg Res 64: 161-169, 1996.
7. Fonti R, Latella G, Bises G, Magliocca F, Nobili F, Caprilli R, and Sambuy Y. Human colonocytes in primary culture: a model to study epithelial growth, metabolism and differentiation. Int J Colorectal Dis 9: 13-22, 1994.
8. Gibson PR, van de Pol E, Maxwell LE, Gabriel A, and Doe WF. Isolation of colonic crypts that maintain structural and metabolic viability in vitro. Gastroenterology 96: 283-291, 1989.
9. Green LM, Reade JL, and Ware CF. Rapid colorimetric assay for cell viability: application to the quantitation of cytotoxic and growth inhibitory lymphokines. J Immunol Methods 70: 257-268, 1984.
10. Grossmann J, Maxson JM, Whitacre CM, Orosz DE, Berger NA, Fiocchi C, and Levine AD. New isolation technique to study apoptosis in human intestinal epithelial cells. Am J Pathol 153: 53-62, 1998.
11. Grossmann J, Mohr S, Lapentina EG, Fiocchi C, and Levine AD. Sequential and rapid activation of select caspases during apoptosis of normal intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol 274: G1117-G1124, 1998.
12. Jobson TM, Billington CK, and Hall IP. Regulation of proliferation of human colonic subepithelial myofibroblasts by mediators important in intestinal inflammation. J Clin Invest 101: 2650-2657, 1998.
13. Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, and Riccardi C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods 139: 271-279, 1991.
14. Olden KW. Diagnosis of irritable bowel syndrome. Gastroenterology 122: 1701-1714, 2002.
15. Panja A. A novel method for the establishment of a pure population of nontransformed human intestinal primary epithelial cell (HIPEC) lines in long term culture. Lab Invest 80: 1473-1475, 2000.
16. Pedersen G, Saermark T, Giese B, Hansen A, Drag B, and Brynskov J. A simple method to establish short-term cultures of normal human colonic epithelial cells from endoscopic biopsy specimens. Comparison of isolation methods, assessment of viability and metabolic activity. Scand J Gastroenterol 35: 772-780, 2000.
17. Potten CS and Loeffler M. Stem cells: attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the crypt. Development 110: 1001-1020, 1990.
18. Rogler G, Daig R, Aschenbrenner E, Vogl D, Schlottmann K, Falk W, Gross V, Scholmerich J, and Andus T. Establishment of long-term primary cultures of human small and large intestinal epithelial cells. Lab Invest 78: 889-890, 1998.
19. Schorkhuber M, Karner-Hanusch J, Sedivy R, Ellinger A, Armbruster C, Schulte-Hermann R, and Marian B. Survival of normal colonic epithelial cells from both rats and humans is prolonged by coculture with rat embryo colonic fibroblasts. Cell Biol Toxicol 14: 211-223, 1998.
20. Strater J, Wedding U, Barth TF, Koretz K, Elsing C, and Moller P. Rapid onset of apoptosis in vitro follows disruption of beta 1- integrin/matrix interactions in human colonic crypt cells. Gastroenterology 110: 1776-1784, 1996.
21. Strater J, Wellisch I, Riedl S, Walczak H, Koretz K, Tandara A, Krammer PH, and Moller P. CD95 (APO-1/Fas)-mediated apoptosis in colon epithelial cells: a possible role in ulcerative colitis. Gastroenterology 113: 160-167, 1997.
22. Usugane M, Fujita M, Lipkin M, Palmer R, Friedman E, and Augenlicht L. Cell proliferation in explant cultures of human colon. Digestion 24: 225-233, 1982.
23. Van de Wetering M, Sancho E, Verweij C, de Lau W, Oving I, Hurlstone A, van der Horn K, Batlle E, Coudreuse D, Haramis AP, Tjon-Pon-Fong M, Moerer P, van den Born M, Soete G, Pals S, Eilers M, Medema R, and Clevers H. The β-catenin/TCF-4 complex imposes a crypt progenitor phenotype on colorectal cancer cells. Cell 111: 241-250, 2002.
24. Whitehead RH, Brown A, and Bhathal PS. A method for the isolation and culture of human colonic crypts in collagen gels. In Vitro Cell Dev Biol 23: 436-442, 1987.
25. Whitehead RH, Demmler K, Rockman SP, and Watson NK. Clonogenic growth of epithelial cells from normal colonic mucosa from both mice and humans. Gastroenterology 117: 858-865, 1999.