Rapid purification of the oxygenase component of toluene dioxygenase from a polyol-responsive monoclonal antibody

Applied and Environmental Microbiology

Volumn 62, Issue 6, 1996, Pages 2133-2137

  Lynch, Nancy A ^a,b   Jiang, Haiyan ^a   Gibson, David T ^a  

^a UNIVERSITY OF IOWA   (United States)

^b UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OXYGENASE; TOLUENE;

ANTIBODY AFFINITY; ARTICLE; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME SUBUNIT; ESCHERICHIA COLI; NONHUMAN; PSEUDOMONAS AERUGINOSA;

EID: 0029934276     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.6.2133-2137.1996     Document Type: Article

References (27)

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3. Collins, K. D., and M. W. Wushabaugh. 1985. The Hofmeister effect and the behaviour of water at interfaces. Q. Rev. Biophys. 18:323-422.
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5. Gibson, D. T., B. Gschwendt, W. K. Yeh, and V. M. Kobal. 1973. Initial reactions in the oxidation of ethylhenzene by Pseudomonas putida. Biochemistry 12:1520-1528.
6. Gibson, D. T., M. Hensley, H. Yoshioka, and T. J. Mabry. 1970. Formation of (+)-cis-2,3-dihydroxy-1-methylcyclohexa-4,6-diene from toluene by Pseudomonas putida. Biochemistry 9:1626-1630.
7. Gibson, D. T., and V. Subramanian. 1984. Microbial degradation of aromatic hydrocarbons, p. 181-252. In D. T. Gibson (ed.), Microbial degradation of organic compounds. Marcel Dekker, New York.
8. Gibson, D. T., W.-K. Yeh, T.-N. Liu, and V. Subramanian. 1982. Toluene dioxygenase: a multicomponent enzyme system from Pseudomonas putida, p. 51-61. In M. Nozaki, S. Yamamoto, Y. Ishimura, M. J. Coon, L. Ernster, and R. W. Estabrook (ed.), Oxygenaecs and oxygen metabolism: a symposium in honor of Osamu Hayaishi. Academic Press, New York.
9. Haddock, J. D., and D. T. Gibson. 1995. Purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400. J. Bacteriol. 177:5834-5839.
10. Harlow, E., and D. Lane. 1988. Antibodies, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
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12. Kobal, V. M., D. T. Gibson, R. E. Davis, and A. Garza. 1973. X-ray determination of the absolute stereochemistry of the initial oxidation product formed from toluene by Pseudomonas putida 39/D. J. Am. Chem. Soc. 95:4420-4421.
13. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
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15. Robertson, J. B., J. C. Spain, J. D. Haddock, and D. T. Gibson. 1992. Oxidation of nitrotoluenes by toluene dioxygenase: evidence for a monooxygenase reaction. Appl. Environ. Microbiol. 58:2643-2648.
16. Sheldrake, G. N. 1992. Biologically derived arene cis-dihydrodiols as synthetic building blocks, p. 127-166. In A. N. Collins, G. N. Sheldrake, and J. Crosby (ed.), Chirality in industry: the commercial manufacture and application of optically active compounds. John Wiley & Sons, Ltd., West Sussex, England.
17. Spain, J. C., G. J. Zylstra, C. K. Blake, and D. T. Gibson. 1989. Monohydroxylation of phenol and 2,5-dichlorophenol by toluene dioxygenase in Pseudomonas putida F1. Appl. Environ. Microbiol. 55:2648-2652.
18. Subramanian, V., T.-N. Liu, W.-K. Yeh, and D. T. Gibson. 1979. Toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography. Biochem. Biophys. Res. Commun. 91:1131-1139.
19. Suen, W.-C., and D. T. Gibson. 1994. Recombinant Escherichia coli strains synthesize active forms of naphthalene dioxygenase and its individual a and β subunits. Gene 143:67-71.
20. Thompson, N. E., D. B. Aronson, and R. R. Burgess. 1990. Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography: elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibody. J. Biol. Chem. 265:7069-7077.
21. Thompson, N. E., D. A. Hager, and R. R. Burgess. 1992. Isolation and characterization of a polyol-responsive monoclonal antibody useful for gentle purification of Escherichia coli RNA polymerase. Biochemistry 31:7003-7008.
22. Wackett, L. P. 1990. Toluene dioxygenase from Pseudomonas putida F1. Methods Enzymol. 188:39-45.
23. Weiss, M., and Z. Eisenstein. 1987. A simple method to optimize elution from affinity chromatography. J. Liq. Chromatogr. 10:2815-2824.
24. Yen, W.-K., D. T. Gibson, and T.-N. Liu. 1977. Toluene dioxygenase: a multicomponent enzyme system. Biochem. Biophys. Res. Commun. 78:401-410.
25. Zylstra, G. J., and D. T. Gibson. 1989. Toluene degradation by Pseudomonas putida F1: nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli. J. Biol. Chem. 264:14940-14946.
26. Zylstra, G. J., and D. T. Gibson. 1991. Aromatic hydrocarbon degradation: a molecular approach, p. 183-203. In V. K. Setlow (ed.), Genetic engineering, principles and methods. Plenum Press, New York.
27. Zylstra, G. J., L. P. Wackett, and D. T. Gibson. 1989. Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes. Appl. Environ. Microbiol. 55:3162-3166.