Mutations in catabolite control protein CcpA showing glucose-independent regulation in Bacillus megaterium

Journal of Bacteriology

Volumn 181, Issue 24, 1999, Pages 7634-7638

  Küster Schöck, Elke ^a,c   Wagner, Andrea ^a   Völker, Uwe ^b   Hillen, Wolfgang ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACILLUS MEGATERIUM; BACTERIAL MUTATION; GENE REPRESSION; GENE TARGETING; GLUCOSE METABOLISM; NONHUMAN; PRIORITY JOURNAL;

BACILLUS MEGATERIUM;

EID: 0345201621     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.24.7634-7638.1999     Document Type: Article

References (35)

1. Beyreuther, K., H. Raufuss, O. Schrecker, and W. Hengstenherg. 1977. The phosphoenolpyruvate-dependent phosphotransferase system of Staphylococcus aureus. I. Amino-acid sequence of the phosphocarrier protein HPr. Eur. J. Biochem. 75:275-286.
2. Davison, S. P., J. D. Santangelo, S. J. Reid, and D. R. Woods. 1995. A Clostridium acetobutylicum regulator gene (regA) affecting amylase production in Bacillus subtilis. Microbiology 141:989-996.
3. Deutscher, J., E. Küster, U. Bergstedt, V. Charrier, and W. Hillen. 1995. Protein kinase-dependcnt HPr/CcpA interaction links glycolytic activity to carbon catabolite repression in Gram-positive bacteria. Mol. Microbiol. 15: 1049-1053.
4. Deutscher, J., and M. H. Saier, Jr. 1983. ATP-dependent protein kinase-catalyzed phosphorylation of a seryl residue in HPr, a phosphate carrier protein of the phosphotransferase system in Streptococcus pyogenes. Proc. Natl. Acad. Sci. USA 80:6790-6794.
5. Egeter, O., and R. Brückner. 1996. Catabolite repression mediated by the catabolite control protein CcpA in Staphylococcus xylosus. Mol. Microbiol. 21:739-749.
6. Friedman, A., T. Fischmann, and T. Steitz. 1995. Crystal structure of lac repressor core tetramer and its implications for DNA looping. Science 268: 1721-1727.
7. Fujita, Y., Y. Miwa, A. Galinier, and J. Deutscher. 1995. Specific recognition of the Bacillus subtilis gnt cis-acting catabolite-responsive element by a protein complex formed between CcpA and seryl-phosphorylated HPr. Mol. Microbiol. 17:953-960.
8. Galinier, A., J. Deutscher, and I. Martin-Verstraete. 1999. Phosphorylation of either Crh or HPr mediates binding of CcpA to the Bacillus subtilis xyn cre and catabolite repression of the xyn operon. J. Mol. Biol. 286:307-314.
9. Gösseringer, R., E. Küster, A. Galinier, J. Deutscher, and W. Hillen. 1997. Cooperative and non-cooperative DNA binding modes of catabolite control protein CcpA from Bacillus megaterium result from sensing two different signals. J. Mol. Biol. 266:665-676.
10. Henkin, T. M. 1996. The role of the CcpA transcriptional regulator in carbon metabolism in Bacillus subtilis. FEMS Microbiol. Lett. 135:9-15.
11. Henkin, T. M., F. J. Grundy, W. L. Nicholson, and G. H. Chambliss. 1991. Catabolite repression of α-amylase gene expression in Bacillus subtilis involves a trans-acting gene product homologous to the Escherichia coli lacl and galR repressors. Mol. Microbiol. 5:575-584.
12. Hueck, C. J., A. Kraus, D. Schmiedel, and W. Hillen. 1995. Cloning, expression and functional analyses of the catabolite control protein CcpA from Bacillus megaterium. Mol. Microbiol. 16:855-864.
13. Jones, B. E., V. Dossonnet, E. Küster, W. Hillen, J. Deutscher, and R. E. Klevit. 1997. Binding of the catabolite repressor protein CcpA to its DNA target is regulated by phosphorylation of its corepressor HPr. J. Biol. Chem. 272:26530-26535.
14. Kim, J. H., Z. T. Guverner, J. V. Cho, K.-C. Chung, and G. H. Chambliss. 1995. Specificity of DNA binding activity of the Bacillus subtilis catabolite control protein CcpA. J. Bacteriol. 177:5129-5134.
15. Kim, J. H., M. I. Voskuil, and G. H. Chambliss. 1998. NADP, corepressor for the Bacillus catabolite control protein CcpA. Proc. Natl. Acad. Sci. USA 95:9590-9595.
16. Kraus, A., and W. Hillen. 1997. Analysis of CcpA mutations defective in carbon catabolite repression in Bacillus megaterium. FEMS Microbiol. Lett. 153:221-226.
17. Kraus, A., E. Küster, A. Wagner, K. Hoffmann, and W. Hillen. 1998. Identification of a corepressor binding site in catabolite control protein CcpA. Mol. Microbiol. 30:955-964.
18. Küster, E., T. Hilbich, M. Dahl, and W. Hillen. 1999. Mutations in catabolite control protein CcpA separating growth effects from catabolite repression. J. Bacteriol. 181:4125-4128.
19. Küster, E., E. J. Luesink, W. M. de Vos, and W. Hillen. 1996. Immunological crossreactivity to catabolite control protein CcpA from Bacillus megaterium is found in many Gram-positive bacteria. FEMS Microbiol. Lett. 139:109-115.
20. Lewis, M., G. Chang, N. Horton, M. Kercher, H. Pace, M. Schumacher, R. Brennan, and P. Lu. 1996. Crystal structure of the lactose operon repressor and its complexes with DNA and inducer. Science 271:1247-1254.
21. Lokman, B. C., M. Heerikhuisen, R. J. Leer, A. van den Broek, Y. Borsboom, S. Chaillou, P. W. Postma, and P. H. Pouwels. 1997. Regulation of expression of the Lactobacillus pentosus xylAB operon. J. Bacteriol. 179:5391-5397.
22. Luesink, E., R. van Herpen, B. Grossiord, O. Kuipers, and W. de Vos. 1998. Transcriptional activation of the glycolytic las operon and catabolite repression of the gal operon in Lactococcus lactis are mediated by the catabolite control protein CcpA. Mol. Microbiol. 30:789-798.
23. Martin-Verstraete, I., J. Deutscher, and A. Galinier. 1999. Phosphorylation of HPr and Crh by HprK, early steps in the catabolite repression signalling pathway for the Bacillus subtilis levanase operon. J. Bacteriol. 181:2966-2969.
24. Miwa, Y., K. Nagura, S. Eguchi, H. Fukuda, J. Deutscher, and Y. Fujita. 1997. Catabolite repression of the Bacillus subtilis gnt operon exerted by two catabolite-responsive elements. Mol. Microbiol. 23:1203-1213.
25. Monedero, V., M. Gosalbes, and G. Perez-Martinez. 1997. Catabolite repression in Lactobacillus casei ATCC 393 is mediated by CcpA. J. Bacteriol. 179:6657-6664.
26. Rygus, T., and W. Hillen. 1992. Catabolite repression of the xyl operon in Bacillus megaterium. J. Bacteriol. 174:3049-3055.
27. Saier, M. H., Jr., T. M. Ramseier, and J. Reizer. 1996. Regulation of carbon utilization, p. 1325-1343. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology, 2nd ed., vol. 1. ASM Press, Washington, D.C.
28. Schumacher, M. A., K. Y. Choi, F. Lu, H. Zalkin, and R. G. Brennan. 1995. Mechanism of corepressor-mediated specific DNA binding by the purine repressor. Cell 83:147-155.
29. Schumacher, M. A., K. Y. Choi, H. Zalkin, and R. G. Brennan. 1994. Crystal structure of LacI member, PurR, bound to DNA: minor groove binding by α helices. Science 266:763-770.
30. Stülke, J., and W, Hillen. 1998. Coupling physiology and gene regulation in bacteria: the phosphotransferase sugar uptake system delivers the signals. Naturwissenschaften 85:583-592.
31. Turinsky, A. J., F. J. Grundy, J. H. Kim, G. H. Chambliss, and T. M. Henkin. 1998. Transcriptional activation of the Bacillus subtilis ackA gene requires sequences upstream of the promoter. J. Bacteriol. 180:5961-5967.
32. Völker, A., H. Antelmann, R. Schmid, A. Schütz, M. Hecker, and U. Völker. Conversion of the 2-D protein index Sub2D of Bacillus subtilis from carrier ampholyte gels to IPG-gels. Submitted for publication.
33. Voskuil, M. I., and G. H. Chambliss. 1996. Significance of HPr and CcpA on catabolite repression of α-amylase. J. Bacteriol. 178:7014-7015.
34. Wagner, A., S. Pöhlmann, and W. Hillen. Unpublished data.
35. Weickert, M. J., and S. Adhya. 1992. A family of bacterial regulators homologous to Gal and Lac repressors. J. Biol. Chem. 267:15869-15874.