Identification of a co-repressor binding site in catabolite control protein CcpA

Molecular Microbiology

Volumn 30, Issue 5, 1998, Pages 955-963

  Kraus, Alexandra ^a   Küster, Elke ^a   Wagner, Andrea ^a   Hoffmann, Karin ^a   Hillen, Wolfgang ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CATABOLITE CONTROL PROTEIN; MUTANT PROTEIN; PROTEIN; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS; BINDING SITE; CATABOLITE REPRESSION; CONTROLLED STUDY; DIMERIZATION; GRAM POSITIVE BACTERIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN FAMILY; PROTEIN FOLDING; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BACILLUS MEGATERIUM; BACTERIAL PROTEINS; BINDING SITES; CARBON; DNA-BINDING PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOSPHOPROTEIN PHOSPHATASE; REPRESSOR PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

BACILLI; BACILLUS MEGATERIUM; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 0031738688     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1998.01123.x     Document Type: Article

References (29)

1. Brent, R., and Ptashne, M. (1981) Mechanism of action of the lexA gene product. Proc Natl Acad Sci USA 78: 4204-4208.
2. Chauvaux, S., Paulsen, I.T., and Saier, M.H. Jr (1998) CcpB, a novel transcription factor implicated in catabolite repression in Bacillus subtilis. J Bacteriol 180: 491-497.
3. Davison, S.P., Santangelo, J.D., Reid, S.J., and Woods, D.R. (1995) A Clostridium acetobutylicum regulator gene (regA) affecting amylase production in Bacillus subtilis. Microbiology 141: 989-996.
4. Deutscher, J., and Saier, M.H. Jr (1983) ATP-dependent protein kinase-catalyzed phosphorylation of a seryl residue in HPr, a phosphate carrier protein of the phosphotransferase system in Streptococcus pyogenes. Proc Natl Acad Sci USA 80: 6790-6794.
5. Deutscher, J., Küster, E., Bergstedt, U., Charrier, V., and Hillen, W. (1995) Protein kinase-dependent HPr/CcpA interaction links glycolytic activity to carbon catabolite repression in Gram-positive bacteria. Mol Microbiol 15: 1049-1053.
6. Fujita, Y., Miwa, Y., Galinier, A., and Deutscher, J. (1995) Specific recognition of the Bacillus subtilis gnt cis-acting catabolite-responsive element by a protein complex formed between CcpA and seryl-phosphorylated HPr. Mol Microbiol 17: 953-960.
7. Galinier, A., Haiech, J., Kilhofer, M.-C., Jaquinod, M., Stülke, J., Deutscher, J., and Martin-Verstraete, I. (1997) The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression. Proc Natl Acad Sci USA 94: 8439-8444.
8. Gösseringer, R., Küster, E., Galinier, A., Deutscher, J., and Hillen, W. (1997) Cooperative and non-cooperative DNA binding modes of catabolite control protein CcpA from Bacillus megaterium result from sensing two different signals. J Mol Biol 266: 665-676.
9. Henkin, T.M. (1996) The role of the CcpA transcriptional regulator in carbon metabolism in Bacillus subtilis. FEMS Microbiol Lett 135: 9-15.
10. Hueck, C.J., and Hillen, W. (1995) Catabolite repression in Bacillus subtilis: a global regulatory mechanism for the Gram-positive bacteria? Mol Microbiol 15: 395-401.
11. Hueck, C.J., Kraus, A., Schmiedel, D., and Hillen, W. (1995) Cloning, expression and functional analyses of the catabolite control protein CcpA from Bacillus megaterium. Mol Microbiol 16: 855-864.
12. Jones, B.E., Dossonnet, V., Küster, E., Hillen, W., Deutscher, J., and Klevit, R.E. (1997) Binding of the catabolite repressor protein CcpA to its DNA target is regulated by phosphorylation of its corepressor HPr. J Biol Chem 272: 26530-26535.
13. Kallipolitis, B.H., Norregaard-Madsen, M., and Valentin-Hansen, P. (1997) Protein-protein communication: structural model of the repression complex formed by CytR and the global regulator CRP. Cell 89: 1101-1109.
14. Kleina, L.G., and Miller, J.H. (1990) Genetic studies of the lac repressor: XIII. Extensive amino acid replacements generated by the use of natural and synthetic nonsense suppressors. J Mol Biol 21: 295-318.
15. Kraus, A., and Hillen, W. (1997) Analysis of CcpA mutations defective in carbon catabolite repression in Bacillus megaterium. FEMS Microbiol Lett 153: 221-226.
16. Küster, E., Luesink, E.J., de Vos, W.M., and Hillen, W. (1996) Immunological Crossreactivity to Catabolite control Protein CcpA from Bacillus megaterium is found in many Gram-positive Bacteria. FEMS Microbiol Lett 139: 109-115.
17. Lewis, M., Chang, G., Horton, N., Kercher, M., Pace, H., Schumacher, M., Brennan, R., and Lu, P. (1996) Crystal structure of the lactose operon repressor and its complexes with DNA and inducer. Science 271: 1247-1254.
18. Markiewicz, P., Klema, L.G., Cruz, C., Ehret, S., and Miller, J.H. (1994) Genetic studies of the lac repressor: XI. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as 'spacers' which do not require a specific sequence. J Mol Biol 240: 421-433.
19. Miwa, Y., Nagura, K., Eguchi, S., Fukuda, H., Deutscher, J., and Fujita, Y. (1997) Catabolite repression of the Bacillus subtilis gnt operon exerted by two catabolite-responsive elements. Mol Microbiol 23: 1203-1213.
20. Postma, P.W., Lengeler, J.W., and Jacobson, G.R. (1993) Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. Microbiol Rev 57: 543-504.
21. Reizer, J., Hoischen, C., Titgemeyer, F., Rivolta, C., Rabus, R., Stülke, J., et al. (1998) A novel protein kinase that controls carbon catabolite repression in bacteria. Mol Microbiol 27: 1157-1169.
22. Rygus, T., and Hillen, W. (1991) Inducible high-level expression of heterologous genes in Bacillus megaterium using the regulatory elements of the xylose-utilization operon. Appl Microbiol Biotechnol 35: 594-599.
23. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring, Harbor Laboratory Press.
24. Schumacher, M.A., Choi, K.Y., Zalkin, H., and Brennan, R.G. (1994) Crystal structure of LacI member, PurR, bound to DNA: minor groove binding by α helices. Science 266: 763-770.
25. Schumacher, M.A., Choi, K.Y., Lu, F., Zalkin, H., and Brennan, R.G. (1995) Mechanism of corepressor-mediated specific DNA binding by the purine repressor. Cell 83: 147-155.
26. Sogaard-Andersen, L., and Valentin-Hansen, P. (1993) Protein-protein interactions in gene regulation: the cAMP-CRP complex sets the specificity of a second DNA-binding protein, the CytR repressor. Cell 75: 557-566.
27. Suckow, J., Markiewicz, P., Kleina, L.G., Miller, J., Kisters-Woike, B., and Müller-Hill, B. (1996) Genetic studies of the Lac repressor. XV: 4000 single amino acid substitutions and analysis of the resulting phenotypes on the basis of the protein structure. J Mol Biol 261: 509-523.
28. Weickert, M.J., and Adhya, S. (1992) A family of bacterial regulators homologous to GaI and Lac Repressors. J Biol Chem 267: 15869-15874.
29. Weisblum, B., Graham, M.Y., Gryczan, T., and Dubnau, D. (1979) Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194. J Bacteriol 137: 635-643.