Orientation and Conformation of Cell-Penetrating Peptide Penetratin in Phospholipid Vesicle Membranes Determined by Polarized-Light Spectroscopy

Journal of the American Chemical Society

Volumn 125, Issue 47, 2003, Pages 14214-14215

  Brattwall, Christina E B ^a   Lincoln, Per ^a   Nordén, Bengt ^a  

^a CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

LIPID; PENETRATIN; PEPTIDE; PEPTIDE NUCLEIC ACID; PHOSPHOLIPID; TRYPTOPHAN DERIVATIVE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CHROMATOPHORE; HYDROPHOBICITY; INTERNALIZATION; MOLECULAR BIOLOGY; NUCLEIC ACID TRANSPORT; PEPTIDE ANALYSIS; POLARIZATION; POLARIZED LIGHT SPECTROSCOPY; PROTEIN DOMAIN; PROTEIN LIPID INTERACTION; PROTEIN STRUCTURE; SPECTROSCOPY; ULTRAVIOLET RADIATION;

CARRIER PROTEINS; CELL MEMBRANE PERMEABILITY; LIGHT; LIPOSOMES; MODELS, MOLECULAR; PHOSPHATIDYLCHOLINES; PHOSPHATIDYLGLYCEROLS; PHOSPHOLIPIDS; PROTEIN CONFORMATION; SPECTRUM ANALYSIS; TRYPTOPHAN;

EID: 0344875239     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0366989     Document Type: Article

References (22)

1. Derossi, D.; Joliot, A.; Chassaing, G.; Prochiantz, A. J. Biol. Chem. 1994, 269, 10444-10450.
2. Allinquant, B.; Hantraye, P.; Mailleux, P.; Moya, K.; Bouillot, C.; Prochiantz, A. J. Cell Biol. 1995, 128, 919-927.
3. Simmons, C. G.; Pitts, A. E.; Mayfield, L. D.; Shay, J. W.; Corey, D. R. Bioorg. Med. Chem. Lett. 1997, 7, 3001-3006.
4. Prochiantz, A. Cur. Opin. Neurobiol. 1996, 6, 629-634.
5. Thorén, P. E. G.; Persson, D.; Isaksson, P.; Goksör, M.; Önfelt, A.; Nordén, B. Biochem. Biophys. Res. Commun. 2003, 307, 100-107.
6. Persson, D.; Thorén, P. E. G.; Herner, M.; Lincoln, P.; Nordén, B. Biochemistry 2003, 42, 421-429.
7. Persson, D.; Thorén, P. E. G.; Nordén, B. FEBS Lett. 2001, 505, 307-312.
8. Magzoub, M.; Eriksson, L. E. G.; Gräslund, A. Biochim. Biophys. Acta-Biomem. 2002, 1563, 53-63.
9. Lindberg, M.; Gräslund, A. FEBS Lett. 2001, 497, 39-44.
10. Berlose, J.; Convert, O.; Derossi, D.; Brunissen, A.; Chassaing, G.; Prochiantz, A. Eur. J. Biochem. 1996, 268, 1304-1314.
11. Bellet-Amalric, E.; Blaudez, D.; Desbat, B.; Graner, F.; Gauthier, F.; Renault, A. Biochim. Biophys. Acta-Biomem. 2000, 1467, 131-143.
12. Salamon, Z.; Lindblom, G.; Tollin, G. Biophys. J. 2003, 84, 1796-1807.
13. Ladokhin, A.; Holloway, P. Biophys. J. 1995, 69, 506-517.
14. POPG/POPC liposomes (20:80 molar ratio) were used throughout this study as a biologically relevant model membrane. A problem due to light scattering by the liposomes has been solved by using sucrose to match the refractive index of the membrane. The spectroscopic technique is here further improved by using synthetic lipids to reduce background absorption.
15. Kilk, K.; Magzoub, M.; Pooga, M.; Eriksson, L. E. G.; Langel, Ü.; Gräslund, A. Bioconjugate Chem. 2001, 12, 911-916.
16. 2 α〉/4), where α is the angle between the transition moment and normal to the membrane plane and S is a factor (0 ≥ S ≤ 1) describing the degree of orientation of the membrane.
17. Czajlik, A. M. E.; Penke, B.; Perczel, A. J. Pept. Sci. 2002, 8, 151-171.
18. Derossi, D.; Calvet, S.; Trembleau, A.; Brunissen, A.; Chassaing, G.; Prochiantz, A. J. Biol. Chem. 1996, 271, 18188-18193.
19. Johansson, L. B.-A.; Lindblom, G.; Nordén, B. Chem. Phys. Lett. 1976, 39, 128-133.
20. Albinsson, B.; Kubista, M.; Nordén, B. J. Phys. Chem. 1989, 93, 6646-6654.
21. Albinsson, B.; Nordén, B. J. Phys. Chem. 1992, 96, 6204-6212.
22. Ardhammar, M.; Lincoln, P.; Nordén, B. Proc. Natl. Acad. Sci. U.S.A. 2002, 99, 15313-15317.