On-column tris(2-carboxyethyl)phosphine reduction and IC5-maleimide labeling during purification of a RpoC fragment on a nickel-nitrilotriacetic acid column

Analytical Biochemistry

Volumn 307, Issue 2, 2002, Pages 368-374

  Bergendahl, Veit ^a   Anthony, Larry C ^a   Heyduk, Tomasz ^b   Burgess, Richard R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b SAINT LOUIS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Drug Discovery; Fluorescence labeling; FRET; High throughput screening; LRET; Maleimide; On column derivatization; RNA polymerase; TCEP; 70

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; DRUG PRODUCTS; ELECTROPHORETIC MOBILITY; ESCHERICHIA COLI; FORSTER RESONANCE ENERGY TRANSFER; NICKEL; PHOSPHORUS COMPOUNDS; PROTEOMICS; PURIFICATION; RNA;

DERIVATIZATIONS; DRUG DISCOVERY; FLUORESCENCE LABELING; FRET; HIGH THROUGHPUT SCREENING; LRET; MALEIMIDES; RNA POLYMERASE; TCEP;

PROTEINS;

EUROPIUM; FLUORESCENT DYE; HISTIDINE; HISTIDINE DERIVATIVE; MALEIMIDE; NICKEL; NITRILOTRIACETIC ACID; PHOSPHINE DERIVATIVE; RNA POLYMERASE; TRIS(2 CARBOXYETHYL)PHOSPHINE; UNCLASSIFIED DRUG;

ARTICLE; BETA CHAIN; CELL INCLUSION; COLUMN CHROMATOGRAPHY; DERIVATIZATION; DRUG SCREENING; ELECTROPHORETIC MOBILITY; ENZYME PURIFICATION; ESCHERICHIA COLI; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN INTERACTION; REDUCTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

EID: 0037102326     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00061-1     Document Type: Article

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