Transfection with different connexin genes alters growth and differentiation of human choriocarcinoma cells

Experimental Cell Research

Volumn 246, Issue 2, 1999, Pages 480-490

  Hellmann, Peter ^a   Grümmer, Ruth ^a   Schirrmacher, Karin ^a   Rook, Martin ^b   Traub, Otto ^c   Winterhager, Elke ^a  

^a UNIVERSITY OF DUISBURG ESSEN   (Germany)

^b UTRECHT UNIVERSITY   (Netherlands)

^c UNIVERSITY OF BONN   (Germany)

Author keywords

Electrophysiology; Gap junctions; Malignant trophoblast; hCG

Indexed keywords

CHORIONIC GONADOTROPIN BETA SUBUNIT; CONNEXIN 26; GAP JUNCTION PROTEIN;

ARTICLE; CELL COMMUNICATION; CELL DIFFERENTIATION; CELL GROWTH; CHORIOCARCINOMA; CONDUCTANCE; CONTROLLED STUDY; ELECTROPHYSIOLOGY; GAP JUNCTION; GENE EXPRESSION; GENETIC TRANSFECTION; GROWTH RATE; HUMAN; HUMAN CELL; PRIORITY JOURNAL;

EID: 0344721484     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.1998.4332     Document Type: Article

References (43)

1. White T. E., Saltzman R. A., Di-Sant'Agnese P. A., Keng P. C., Sutherland R. M., Miller R. K. Human choriocarcinoma (JAr) cells grown as multicellular spheroids. Placenta. 9:1988;583-598.
2. John N. J., Linke M., Denker H.-W. Quantitation of human choriocarcinoma spheroid attachment to uterine epithelial cell monolayers. In Vitro Cell Dev. Biol. 29A:1993;461-468.
3. Grümmer R., Hohn H.-P., Mareel M. M., Denker H.-W. Adhesion and invasion of three human choriocarcinoma cell lines into human endometrium in a three-dimensional organ culture system. Placenta. 15:1994;411-429.
4. Goodenough D. A., Goliger J. A., Paul D. L. Connexins, connexons, and intercellular communication. Annu. Rev. Biochem. 65:1996;475-502.
5. Kumar N. M., Gilula N. B. The gap junction communication channel. Cell. 84:1996;381-388.
6. Steinberg T. H., Civitelli R., Geist S. T., Robertson A. J., Hick E., Veenstra R. D., Wang H. Z., Warlow P. M., Westphale E. M., Laing J. G., Beyer E. C. Connexin43 and connexin45 form gap junctions with different molecular permeabilities in osteoblastic cells. EMBO J. 13:1994;744-750.
7. Elfgang C., Eckert R., Lichtenberg-Frate H., Butterweck A., Traub O., Klein R. A., Hülser D. F., Willecke K. Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa cells. Cell Biol. 129:1995;805-817.
8. Kwak B. R., Hermans M. M., De Jonge H. R., Lohmann S. M., Jongsma H. J., Chanson M. Differential regulation of distinct types of gap junction channels by similar phosphorylating conditions. Mol. Biol. Cell. 6:1995;1707-1719.
9. Veenstra R. D. Size and selectivity of gap junction channels formed from different connexins. J. Bioenerg. Biomembr. 28:1996;327-335.
10. Hellmann P., Winterhager E., Spray D. C. Properties of connexin40 gap junction channels endogenously expressed and exogenously overexpressed in human choriocarcinoma cell lines. Pflügers Arch. 432:1996;501-509.
11. Hellmann P., von Ostau C., Grümmer R., Winterhager E. Connexin40 expression in the human trophoblast: Implicator for proliferation and invasion properties. Placenta. 16:1995;A.26.
12. Kaufmann P., Castellucci M. Extravillous trophoblast in the human placenta. Trophoblast Res. 10:1997;21-65.
13. Patillo R. A., Hussa R. O., Huang W. Y., Delfs E., Mattingly R. F. Estrogen production by trophoblastic tumors in tissue culture. J. Clin. Endocrinol. 34:1972;59-61.
14. Burres N. S., Cass C. E. The effects of hypoxanthine on methotrexate-induced differentiation of cultured human choriocarcinoma (BeWo) cells. Biochem. Cell Biol. 64:1986;811-815.
15. Lee S. W., Tomasetto C., Paul D., Keyomarsi K., Sager R. Transcriptional downregulation of gap-junction proteins blocks junctional communication in human mammary tumor cell lines. J. Cell Biol. 118:1992;1213-1221.
16. Wilgenbus K. K., Kirkpatrick C. J., Knüchel R., Willecke K., Traub O. Expression of Cx26, Cx32 and Cx43 gap junction proteins in normal and neoplastic human tissues. Int. J. Cancer. 51:1992;522-529.
17. Yamasaki H., Naus C. C. G. Role of connexin genes in growth control. Carcinogenesis. 17:1996;1199-1213.
18. Eghbali B., Kessler J. A., Reid L. M., Roy C., Spray D. C. Involvement of gap junctions in tumorigenesis: Transfection of tumor cells with connexin 32 cDNA retards growth in vivo. Proc. Natl. Acad. Sci. USA. 88:1991;10701-10705.
19. Zhu D., Kidder G. M., Caveney S., Naus C. C. Growth retardation in glioma cells cocultured with cells overexpressing a gap junction protein. Proc. Natl. Acad. Sci. USA. 89:1992;10218-10221.
20. Bond S. L., Bechberger J. F., Khoo N. K., Naus C. C. Transfection of C6 glioma cells with connexin32: The effects of expression of a nonendogenous gap junction protein. Cell Growth Differ. 5:1994;179-186.
21. Mesnil M., Krutovskikh V., Piccoli C., Elfgang C., Traub O., Willecke K., Yamasaki H. Negative growth control of HeLa cells by connexin genes: Connexin species specificity. Cancer Res. 55:1995;629-639.
22. Zhang J. T., Nicholson B. J. Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA. J. Cell Biol. 109:1989;3391-3401.
23. Beyer E. C., Paul D. L., Goodenough D. A. A protein from rat heart homologous to gap junction protein from liver. J. Cell Biol. 105:1987;2621-2629.
24. Haefliger J. A., Bruzzone R., Jenkins N. A., Gilbert D. J., Copeland N. G., Paul D. L. Four novel members of the connexin family of gap junction proteins. J. Biol. Chem. 267:1992;2057-2064.
25. Moos M., Gallwitz D. Structure of two human β-actin-related processed genes one of which is located next to simple repetitive sequence. EMBO J. 2:1983;757-761.
26. Winterhager E., Stutenkemper R., Traub O., Beyer E., Willecke K. Expression of different connexin genes in rat uterus during decidualization and at term. Eur. J. Cell Biol. 55:1991;133-142.
27. Traub O., Eckert R., Lichtenberg-Frate H., Elfgang C., Bastide B., Scheidtmann K. H., Hülser D. F., Willecke K. Immunochemical and electrophysiological characterization of murine connexin40 and -43 in mouse tissues and transfected human cells. Eur. J. Cell Biol. 64:1994;101-112.
28. Traub O., Look J., Dermietzel R., Brümmer F., Hülser D., Willecke K. Comparative characterization of the 21 kD and 26 kD gap junction proteins in murine liver and cultured hepatocytes. J. Cell Biol. 08:1989;1039-1051.
29. Neyton J., Trautmann A. Single-channel currents of an intercellular junction. Nature. 317:1985;331-335.
30. Grümmer R., Hellmann P., Traub O., Soares M. J., El-Sabban M. E., Winterhager E. Regulation of connexin31 gene expression upon retinoic acid treatment in rat choriocarcinoma cells. Exp. Cell Res. 227:1996;23-32.
31. Gros B. D., Jongsma H. J. Connexin in mammalian heart function. BioEssays. 18:1996;719-730.
32. Chen S. C., Pelletier D. B., Ao P., Boynton A. L. Connexin43 reverses the phenotype of transformed cells and alters their expression of cyclin/cyclin-dependent kinases. Cell Growth Differ. 6:1995;681-690.
33. Hirschi K. K., Xu C. E., Tsukamoto T., Sager R. Gap junction genes Cx26 and Cx43 individually suppress the cancer phenotype of human mammary carcinoma cells and restore differentiation potential. Cell Growth Differ. 7:1996;861-870.
34. Krutovskikh V., Mazzoleni G., Mironov N., Omori Y., Aguelon A. M., Mesnil M., Berger F., Partensky C., Yamasaki H. Altered homologous and heterologous gap-junctional intercellular communication in primary human liver tumors associated with aberrant protein localization but not gene mutation of connexin 32. Int. J. Cancer. 56:1994;87-94.
35. Yamasaki H., Mesnil M., Omori Y., Mironov N., Krutovskikh V. Intercellular communication and carcinogenesis. Mutat. Res. 333:1995;181-188.
36. Trosko J. E., Chang C. C., Madhukar B. V. Symposium: Cell communication in normal and uncontrolled growth. Radiat. Res. 123:1990;241-251.
37. Temme A., Buchmann A., Gabriel H.-D., Nelles E., Schwarz M., Willecke K. High incidence of spontaneous and chemically induced liver tumors in mice deficient for connexin32. Curr. Biol. 7:1997;713-716.
38. Spray D. C. Physiological and pharmacological regulation of gap junction. Molecular Mechanisms of Epithelial Cell Junctions: From Development to Disease. 1994;R. G. LandesMedical Intelligence Unity, Austin. p. 195-215.
39. Stoker M. G. P. Transfer of growth inhibition between normal and virus-transformed cells: Autoradiographic studies using marked cells. J. Cell Sci. 2:1967;293-304.
40. Loewenstein W. The cell-to-cell channels of gap junctions. Cell. 48:1988;725-726.
41. Pierce G. B., Midgley A. R. The origin and function of human syncytiotrophoblast giant cells. Am. J. Pathol. 43:1963;153-173.
42. Kliman H. J., Nestler J. E., Sermasi E., Sauger J. M., Strauss J. F. III. Purification, characterization, and in vitro differentiation of cytotrophoblasts from human term placentae. Endocrinology. 118:1986;1567-1582.
43. Kohler P. O., Bridson E. Isolation of hormone-producing clonal lines of human choriocarcinoma. J. Clin. Endocrinol. 32:1971;683-687.