Recent advances in the use of neurotropic viruses for circuit analysis

Current Opinion in Neurobiology

Volumn 13, Issue 5, 2003, Pages 603-606

  Enquist, Lynn W ^a   Card, J Patrick ^b  

^a PRINCETON UNIVERSITY   (United States)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRE RECOMBINASE; GREEN FLUORESCENT PROTEIN;

BRAIN SLICE; CELL STRUCTURE; CYTOARCHITECTURE; GENE EXPRESSION; NERVE CELL NETWORK; NERVE SPROUTING; NEUROPHYSIOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PSEUDORABIES HERPETOVIRUS; REPORTER GENE; REVIEW; SYNAPTIC TRANSMISSION; VIRUS ASSEMBLY; VIRUS INFECTION; VIRUS RECOMBINANT; VIRUS REPLICATION;

EID: 0344445373     PISSN: 09594388     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.conb.2003.08.001     Document Type: Review

References (23)

1. Kristensson K. Sorting signals and targeting of infectious agents through axons: an annotation to the 100 years' birth of the name "axon" Brain Res Bull. 41:1996;327-333.
2. Enquist L.W., Husak P.J., Banfield B.W., Smith G.A. Infection and spread of α-herpes viruses in the nervous system. Adv Virus Res. 51:1999;237-347.
3. Mettenleiter TC: Molecular properties of α-herpes viruses used in transneuronal pathway tracing. In Viral Vectors: Gene Therapy and Neuroscience Applications. Edited by Kaplitt MG, Loewy AD. Academic Press; 1995:367-393.
4. Card J.P. Pseudorabies virus neuroinvasiveness: a window into the functional organization of the brain. Adv Virus Res. 56:2001;39-71.
5. Loewy A.D. Viruses as transneuronal tracers for defining neural circuits. Neurosci Biobehav Rev. 22:1998;679-684.
6. Jansen A.S.P., Van Nguyen X., Karpitskiy V., Mettenleiter T.C., Loewy A.D. Central command neurons of the sympathetic nervous system: basis of the fight-or-flight response. Science. 270:1995;253-260.
7. Kim J.-S., Moore R.Y., Enquist L.W., Card J.P. Circuit-specific co-infection of neurons in the rat central nervous system with two pseudorabies virus recombinants. J Virol. 75:1999;9521-9531.
8. Mabon P.J., Weaver L.C., Dekaban G.A. Cyclosporin A reduces the inflammatory response to a multi-mutant herpes simplex virus type-1 leading to improved transgene expression in sympathetic neurons in hamsters. J Neurovirol. 5:1999;268-279.
9. Travers J.B., Rinaman L. Identification of lingual motor control circuits using two strains of pseudorabies virus. Neuroscience. 115:2002;1130-1131.
10. Ueyama T., Krout K.E., Van Nguyen X., Karpitskiy V., Kollert A., Mettenleiter T.C., Loewy A.D. Suprachiasmatic nucleus: a central autonomic clock. Nat Neurosci. 2:1999;1051-1053.
11. Billig I., Foris J.M., Enquist L.W., Card J.P., Yates B.J. Definition of neuronal circuitry controlling the activity of phrenic and abdominal motoneurons in the ferret using recombinant strains of pseudorabies virus. J Neurosci. 20:2000;7446-7454.
12. Billig I., Hartge K., Card J.P., Yates B.J. Transneuronal tracing of neural pathways controlling abdominal musculature in the ferret. Brain Res. 912:2001;24-32.
13. Krout K.E., Mettenleiter T.C., Loewy A.D. Single CNS neurons link both central motor and cardiosympathetic systems: a double-virus tracing study. Neuroscience. 118:2003;853-866 This study, and that of Kerman and colleagues [14], uses dual transneuronal tracing with PRV recombinants to identify a population of CNS neurons that coordinate autonomic and motor function.
14. Kerman I., Enquist L.W., Watson S.J., Yates B.J. Brainstem substrates of central command circuitry identified using transsynaptic tracing with pseudorabies virus recombinants. J Neurosci. 22:2003;4657-4666.
15. Cano G, Card JP, Sved AF: Dual viral transneuronal tracing of central autonomic circuits involved in the innervation of the two kidneys in the rat. J Comp Neurol 2003, in press.
16. Billig I., Card J.P., Yates B.J. Neurochemical phenotypes of MRF neurons influencing diaphragm and rectus abdominis activity. J Appl Physiol. 94:2003;391-398 Dual transneuronal tracing with recombinant strains of PRV is combined with characterization of neuronal phenotype in this analysis of medial reticular formation neurons that collateralize to innervate motor neurons innervating diaphragm and rectus-abdominus muscles.
17. Buijs R.M., La Fleur S.E., Wortel J., Van Heyningen C., Zuiddam L., Mettenleiter T.C., Kalsbeek A., Magai K., Niijima A. The suprachiasmatic nucleus balances sympathetic and parasympathetic output to peripheral organs through separate preautonomic neurons. J Comp Neurol. 464:2003;36-48.
18. Card J.P., Dubin J.R., Whealy M.E., Enquist L.W. Influence of infectious dose upon productive replication and transynaptic passage of pseudorabies virus in rat central nervous system. J Neurovirol. 1:1995;349-358.
19. Card J.P., Enquist L.W., Moore R.Y. Neuroinvasiveness of pseudorabies virus injected intracerebrally is dependent on viral concentration and terminal field density. J Comp Neurol. 407:1999;438-452.
20. Davis S.F., Williams K.W., Xu W.Y., Glatzer N.R., Smith B.N. Selective enhancement of synaptic inhibiton by hypocretin (orexin) in rat vagal motor neurons: implications for autonomic regulation. J Neurosci. 23:2003;3844-3854.
21. ••].
22. ••], provides the proof-of-principle demonstration that EGFP expression of PRV recombinants can be used to identify components of a neural circuit in live slices of brain for electrophysiological characterization.
23. DeFalco J., Tomishima M.J., Liu H., Zhao C., Cai X., Marth J.D., Enquist L.W., Friedman J.M. Virus-assisted mapping of neural inputs to a feeding center in the hypothalamus. Science. 291:2001;2608-2613 This important contribution provides the proof-of-principle demonstration that the Cre recombinase system can be exploited for conditional replication of PRV in neuronal circuit analysis.