Use of Recombinant Cyprosin in the Manufacture of Ewe's Milk Cheese

Journal of Agricultural and Food Chemistry

Volumn 51, Issue 25, 2003, Pages 7426-7430

  Fernández Salguero, José ^a   Prados, Francisco ^a   Calixto, Filomena ^a   Vioque, Montserrat ^a   Sampaio, Pedro ^a   Tejada, Luis ^a  

^a UNIVERSITY OF CÓRDOBA   (Spain)

Author keywords

Ovine cheese; Proteolysis; Recombinant cyprosin; Vegetable coagulant

Indexed keywords

COAGULATING AGENT; CYPROSIN; NITROGEN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CARDOON; CATTLE; CHEESE; CHEESEMAKING; FOOD PROCESSING; MICROBIOLOGICAL PARAMETERS; MILK; NONHUMAN; PH; PROTEIN DEGRADATION; SHEEP;

ANIMALS; ASPARTIC ENDOPEPTIDASES; CATTLE; CHEESE; CHYMOSIN; CYNARA; FEMALE; FOOD HANDLING; MILK; RECOMBINANT PROTEINS; SHEEP;

ANIMALIA; BOS TAURUS; CYNARA; CYNARA CARDUNCULUS; OVIS; OVIS ARIES;

EID: 0344198047     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf034573h     Document Type: Article

References (30)

1. Fox, P.; McSweeney, P. L. H. Proteolysis in cheese during ripening. Food Rev. Int. 1996, 12, 3.1-3.56.
2. Carr, S. Pocket Guide to Cheese; Mitchell Beazley Publishing: London, U.K., 1981.
3. Fernández-Salguero, J.; Sanjuán, E. Influence of vegetable and animal rennet on proteolysis during ripening in ewes' milk cheese. Food Chem. 1999, 64, 177-183.
4. Macedo, A. C.; Malcata, F. X.; Oliveira, J. C. The technology, chemistry and microbiology of Serra cheese: A review. J. Dairy Sci. 1993, 76, 1725-1739.
5. Fernández-Salguero, J.; Tejada, L.; Gómez, R. Use of powdered vegetable coagulant in the manufacture of ewe's milk cheeses. J. Sci. Food Agric. 2002, 82, 464-468.
6. Heimgartner, U.; Pietrzak, M.; Geertsen, R.; Brodelius, P.; Silva Figueiredo, A. C.; Pais, M. S. Purification and partial characterization of milk clotting proteases from flowers of Cynara cardunculus. Phytochemistry 1990, 29, 1405-1410.
7. Cordeiro, M.; Jacob, E.; Puhan, Z.; Pais, M. S.; Brodelius, P. Milk clotting and proteolytic activities of purified cynarases from Cynara cardunculus: A comparison to chymosin. Milchwissenschaft 1992, 47, 683-700.
8. Cordeiro, M.; Pais, M. S.; Brodelius, P. Tissue-specific expression of multiple forms of cyprosin (aspartic proteinase) in flowers of Cynara cardunculus. Physiol. Plant. 1994, 92, 645-653.
9. Vioque, M.; Gómez, R.; Sánchez, E.; Mata, C.; Tejada, L.; Fernández-Salguero, J. Chemical and microbiological characteristics of ewes' milk cheese manufactured with extracts from flowers of Cynara cardunculus and Cynara humilis as coagulants. J. Agric. Food Chem. 2000, 48, 451-456.
10. Fernández-Salguero, J.; Sánchez, E.; Gómez, R.; Mata, C.; Vioque, M.; Tejada, L. A preliminary study of microbiological quality of cardoons of the genus Cynara L. used in manufacture of traditional cheeses. Michwissenschaft 1999, 54, 688-690.
11. Gómez, R.; Sánchez, E.; Vioque, M.; Ferreira, J.; Tejada, L.; Fernández-Salguero, J. Microbiological characteristics of ewes' milk cheese manufactured using aqueous extracts of flowers from various species of cardoon Cynara L. Milchwissenschaft 2001, 56, 16-19.
12. Brodelius, P.; Cordeiro, M.; Pais, M. S. Aspartic proteinase (Cyprosins) from Cynara cardunculus spp. flavescens cv. cardoon: purification, characterization and tissue-specific expression. In Aspartic Proteinases (Takahashi, K., Ed.) Plenum Press: New York, 1995; 255 pp.
13. Planta, R. J.; Calixto, F.; Pais, M. S. Production by yeast of aspartic proteinases from plant origin with sheep's, cow's, goat's milk, etc. clotting and proteolytic activity. Patent EP1196542 (WO0075283), Lisboa, Portugal, 2000.
14. Fernández-Salguero, J.; Sanjuán, E.; Montero, E. A preliminary study of the chemical composition of Guía cheese. J. Food Compos. Anal. 1991, 4, 262-269.
15. Fernández-Salguero, J.; Marcos, A.; Alcalá, M.; Esteban, M. A. Proteolysis of Cabrales cheese and other European blue vein cheese varieties. J. Dairy Res. 1989, 56, 141-146.
16. Folkertsma, B.; Fox, P. F. Use of Cd-ninhydrin reagent to assess proteolysis in cheese during ripening. J. Dairy Res. 1992, 217-224.
17. APHA. Standard Methods for the Examination of Dairy Products, 15th ed.; American Public Health Association: Washington, DC, 1984.
18. Buttiaux R. Manual de Techniques Bactériologiques, 4th ed.; Flamarion Medicine Science: Paris, France, 1974.
19. SAS. General Linear Models (GLMs) Procedures. In SAS/STAT User's Guide: Statistics, 4th ed.; SAS Institute: Cary, NC, 1989.
20. Nuñez, M.; Fernández del Pozo, B.; Rodríguez Marín, M. A.; Gaya, P.; Medina, M. Effect of vegetable and animal rennet on chemical, microbiological, rheological and sensory characteristics of La Serena cheese. J. Dairy Res. 1991, 58, 511-519.
21. Tejada, L.; Fernández-Salguero, J. Chemical and microbiological characteristics of ewe milk cheese (Los Pedroches) made with a powdered vegetable coagulant or calf rennet. Ital. J. Food Sci. 2003, 15, 125-131.
22. Sousa, M. J.; Malcata, F. X. Comparison of plant and animal rennets in terms of microbiological, chemical and proteolysis characteristics of ovine cheese. J. Agric. Food Chem. 1997, 45, 74-81.
23. Law, B. A.; Wigmore, A. Accelerated cheese ripening with food grade proteinases. J. Dairy Res. 1982, 49, 301-308.
24. McSweeney, P. L. H.; Fox, P. F. Cheese: methods of chemical analysis. In Cheese: Chemistry, Physics, and Microbiology, 2nd ed.; Fox, P. F., Ed.; Chapman and Hall: London, U.K., 1993; Vol. I.
25. Carmona, M. A.; Sanjuán, E.; Gómez, R.; Fernández-Salguero, J. Effect of starter cultures on the physico-chemical and biochemical features in ewe cheese made with extracts from flowers of Cynara cardunculus L. J. Sci. Food Agric. 1999, 79, 737-744.
26. Fernández del Pozo, B.; Gaya, P.; Medina, M.; Rodríguez Marín, M. A.; Nuñez, M. Changes in chemical and rheological characteristics of La Serena ewe's milk cheese during ripening. J. Dairy Res. 1988, 55, 457-464.
27. Sousa, M. J.; Malcata, F. X. Influence of pasteurization of milk and addition of starter cultures on protein breakdown in ovine cheeses manufactured with extracts from flowers of Cynara cardunculus. Food Chem. 1997, 57, 549-556.
28. Poullet, B.; Huertas, M.; Sánchez, A.; Cáceres, P.; Larriba, G. Microbial study of Casar de Cáceres throughout ripening. J. Dairy Res. 1991, 58, 231-238.
29. Tatini, S. R.; Jezeski, J. J.; Morris, H. A.; Olson, J. C.; Casman, E. P. Production of staphylococcal enterotoxin A in Cheddar and Colby cheese. J. Dairy Sci. 1971, 54, 815-825.
30. Medina, M.; Fernández del Pozo, B.; Rodríguez Marín, M. A.; Gaya, P.; Núñez, M. Effect of lactic starter inoculation on chemical, microbiological, rheological and sensory characteristics of La Serena cheese. J. Dairy Res. 1991, 58, 355-361.