Fluorescence microscopy: A powerful technique to detect low GUS activity in vascular tissues

Plant Journal

Volumn 33, Issue 1, 2003, Pages 205-209

  Rech, Philippe ^a   Grima Pettenati, Jacqueline ^a   Jauneau, Alain ^a  

^a Centre National de la Recherche Scientifique   (France)

Author keywords

Cinnamyl alcohol dehydrogenase; Confocal microscopy; Fluorescence microscopy; GUS; Transcriptional regulation; Xylem

Indexed keywords

FLUORESCENCE; GENES; MICROSCOPIC EXAMINATION; TISSUE;

PROMOTER DELETION ANALYSIS;

PLANTS (BOTANY);

EUCALYPTUS; GLUCURONIDASE; HISTOCYTOCHEMISTRY; MICROSCOPY, FLUORESCENCE; MUTAGENESIS; RECOMBINANT FUSION PROTEINS; SENSITIVITY AND SPECIFICITY; SEQUENCE DELETION;

ARABIDOPSIS; EMBRYOPHYTA; EUCALYPTUS GUNNII; NICOTIANA TABACUM; POPULUS BEROLINENSIS; VITIS;

EID: 0037237681     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.2003.016017.x     Document Type: Review

References (16)

1. Beeckman, T. and Engler, G. (1994) An easy technique for the clearing of histochemically stained plant tissue. Plant Mol. Biol. Report, 12, 37-42.
2. Bevan, M. (1984) Binary Agrobacterium vectors for plant transformation. Nucl. Acids Res. 12, 8711-8721.
3. Feuillet, C., Lauvergeat, V., Deswarte, C., Pilate, G., Boudet, A. and Grima-Pettenati, J. (1995) Tissue- and cell-specific expression of a cinnamyl alcohol dehydrogenase promoter in transgenic poplar plants. Plant Mol. Biol. 27, 651-667.
4. Fleming, A.J., Manzara, T., Gruissem, W. and Kuhlemeier, C. (1996) Fluorescent imaging of GUS activity and RT-PCR analysis of gene expression in the shoot apical meristem. Plant J. 10, 745-754.
5. Hawkins, S., Pilate, G., Duverger, E., Boudet, A.M. and Grima-Pettenati, J. (2002) The use of GUS histochemistry to visualise lignification gene expression in situ during wood formation. In: Wood Formation in Trees (Chaffey, N., ed.). Singapore: Taylor & Francis Group, pp. 271-295.
6. Hawkins, S., Samaj, J., Lauvergeat, V., Boudet, A.M. and Grima-Pettenati, J. (1997) Cinnamyl alcohol dehydrogenase: identification of new sites of promoter activity in transgenic poplar. Plant Physiol. 113, 321-325.
7. Hoekema, A., Hirsch, P.R., Hooykaas, P.J.J. and Schilperoort, R.A. (1983) A binary plant vector strategy based on separation of virand T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature, 303, 179-180.
8. Holsters, M. (1978) Transfection and transformation of Agrobacterium tumefaciens. Mol. Gen. Genet. 163, 181-187.
9. Horsch, R.B., Fry, J.E., Hoffmann, N.L., Eichholtz, D., Rogers, S.G. and Fraley, R.T. (1985) A simple and general method for transferring genes into plants. Science, 227, 1229-1231.
10. Jefferson, R.A. (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Report, 5, 387-405.
11. Lauvergeat, V., Rech, P., Jauneau, A., Guez, C., Coutos-Thevenot, P. and Grima-Pettenati, J. (2002) The vascular expression pattern directed by the Eucalyptus gunnii cinnamyl alcohol dehydrogenase EgCAD2 promoter is conserved among woody and herbaceous plant species. Plant Mol. Biol. 50, 497-509.
12. Piquemal, J., Lapierre, C., Myton, K., O'Connell, A., Schuch, W., Grima-Pettenati, J. and Boudet, A.M. (1998) Downregulation of cinnamoyl-CoA reductase induces significant changes of lignin profiles in transgenic tobacco plants. Plant J. 13, 71-83.
13. Samaj, J., Hawkins, S., Lauvergeat, V., Grima-Pettenati, J. and Boudet, A. (1998) Immunolocalization of cinnamyl alcohol dehydrogenase 2 (CAD2) indicates a good correlation with cell-specific activity of CAD2 promoter in transgenic poplar shoots. Planta, 204, 437-443.
14. Shen, W.H., Escudero, J., Schlappi, M., Ramos, C., Hohn, B. and Koukolikova-Nicola, Z. (1993) T-DNA transfer to maize cells: histochemical investigation of beta-glucuronidase activity in maize tissues. Proc. Natl. Acad. Sci. USA, 90, 1488-1492.
15. Sheppard, C.J.R. and Shotton, D.M. (1997) Confocal Laser Scanning Microscopy. Singapore: Springer-Verlag.
16. Zhou, M., Upson, R.H., Diwu, Z. and Haugland, R.P. (1996) A fluorogenic substrate for beta-glucuronidase: applications in fluorometric, polyacrylamide gel and histochemical assays. J. Biochem. Biophys. Meth. 33, 197-220.