DNA effects in repair-deficient V79 Chinese hamster cells studied with the comet assay

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 377, Issue 2, 1997, Pages 279-286

  Heibig, Rainer ^a,b   Speit, Günter ^a  

^a UNIVERSITY OF ULM   (Germany)

^b CCR Cytotest Cell Research   (Germany)

Author keywords

Cytotoxicity; DNA strand break; Genotoxicity; Methyl methanesulfonate; Neocarzinostatin; Single cell (SCG) assay

Indexed keywords

MESYLIC ACID METHYL ESTER; ZINOSTATIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL LINE; CELL MUTANT; CONTROLLED STUDY; CYTOTOXICITY; DNA REPAIR; DNA STRAND BREAKAGE; DOSE RESPONSE; GEL ELECTROPHORESIS; GENOTOXICITY; HAMSTER; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; ANTINEOPLASTIC AGENTS, ALKYLATING; CELL LINE; CELL SURVIVAL; CRICETINAE; DNA DAMAGE; DNA REPAIR; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS; METHYL METHANESULFONATE; MUTAGENS; NUCLEIC ACID SYNTHESIS INHIBITORS; TIME FACTORS; ZINOSTATIN;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0030858828     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0027-5107(97)00087-0     Document Type: Article

References (32)

1. A.R. Collins, Mutant rodent cell lines sensitive to ultraviolet light, ionizing radiation and cross-linking agents: a comprehensive survey of genetic and biochemical characteristics, Mutation Res. 239 (1993) 99-118.
2. M.Z. Zdzienicka, Mammalian mutants defective in the response to ionizing radiation-induced DNA damage, Mutation Res. 336 (1995) 203-213.
3. V.J. McKelvey-Martin, M.H.L. Green, P. Schmezer, B.L. Pool-Zobel, M.P. De Meo, A. Collins, The single cell gel electrophoresis assay (comet assay): a European review, Mutation Res. 288 (1993) 7-63.
4. D.W. Fairbairn, P.L. Olive, K.L. O'Neill, The comet assay: a comprehensive review, Mutation Res. 339 (1995) 37-59.
5. P.A. Jeggo, Studies on mammalian mutants defective in rejoining double-strand breaks in DNA, Mutation Res. 239 (1990) 1-16.
6. M.Z. Zdzienicka, Q. Tran, G.P. van der Schans, J.W.I.M. Simons, Characterization of an X-ray-hypersensitive mutant of Chinese hamster cells, Mutation Res. 194 (1988) 239-249.
7. R. Helbig, M.Z. Zdzienicka, G. Speit, The effect of defective DNA double-strand break repair on mutations and chromosome aberrations in the Chinese hamster cell mutant XR-V15B, Radiation Res. 143 (1995) 151-157.
8. F. Pergola, M.Z. Zdzienicka, M.R. Lieber, V(D)J recombination in mammalian cell mutants defective in DNA double strand break repair, Mol. Cell. Biol. 13 (1993) 3464-3471.
9. G.E. Taccioli, G. Rathbun, E. Oltz, T. Stamato, P.A. Jeggo, F.W. Alt, Impairment of V(D)J recombination in double-strand break repair mutants, Science 260 (1993) 207-210.
10. G.E. Taccioli, T.M. Gottlieb, T. Blunt, A. Priestley, J. Demengeot, R. Mizuta, A.R. Lehman, F.W. Alt, S. Jackson, P.A. Jeggo, Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination, Science 265 (1994) 1442-1445.
11. V. Smider, W.K. Rathmell, M.R. Lieber, G. Chu, Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA, Science 266 (1994) 288-291.
12. M. Hafezparast, G.P. Kaur, M.Z. Zdzienicka, R.S. Athwal, A.R. Lehmann, P.A. Jeggo, Subchromosomal localization of a gene (XRCC5) involved in double strand break repair to the region 2q34-36, Somat. Cell. Mol. Genet. 19 (1993) 413-421.
13. D.J. Chen, B.L. Marrone, T. Nguyen, M. Stackhouse, Y. Zhao, M.J. Siciliano, Regional assignment of a human DNA repair gene (XRCC5) to 2q35 by X-ray hybrid mapping, Genomics 21 (1994) 423-427.
14. M.Z. Zdzienicka, N.G.J. Jaspers, G.P. van der Schans, A.T. Natarajan, J.W.I.M. Simons, Ataxia-telangiectasia-like Chinese hamster V79 cell mutants with radioresistant DNA synthesis, chromosomal instability, and normal DNA strand break repair, Cancer Res. 49 (1989) 1481-1485.
15. W. Jongmans, G.W.C.T. Verhaegh, K. Sankaranarayanan, P.H.M. Lohman, M.Z. Zdzienicka, Cellular characteristics of Chinese hamster cell mutants resembling ataxia-telangiectasia cells, Mutation Res. 294 (1993) 207-214.
16. Zdzienicka, M.Z., J.W.I.M. Simons, P.H.M. Lohman, Chinese hamster cell lines defective in DNA repair, in: M.W. Lambert and J. Laval (Eds.), DNA Repair Mechanisms and Their Biological Implications in Mammalian Cells, Plenum, New York, 1985, pp. 535-545.
17. R. Helbig, E. Gerland, M.Z. Zdzienicka, G. Speit, The pattern of mutations induced by neocarzinostatin and methyl methanesulfonate in the ataxia telangiectasia-like Chinese hamster cell line V-E5, Mutation Res. 336 (1995) 307-316.
18. I.H. Goldberg, Mechanism of neocarzinostatin action: role of DNA microstructure in determination of chemistry of bistranded oxidative damage, Acc. Chem. Res. 24 (1991) 191-198.
19. L.F. Povirk, DNA damage and mutagenesis by radiomimetic DNA-cleaving agents: bleomycin, neocarzinostatin and other enediynes, Mutation Res. 355 (1996) 71-89.
20. R. Mirzayans, M. Liuzzi, M.C. Paterson, Methylmethane-sulfonate-induced DNA damage and its repair in cultured human fibroblasts: normal rates of induction and removal of alkali-labile sites in xeroderma pigmentosum (group A) cells, Carcinogenesis 9 (1988) 2257-2263.
21. G.H.S. Strauss, Non-random cell killing in cryopreservation: implications for performance of the battery of leukocyte tests (BLT) I. Toxic and immunotoxic effects, Mutation Res. 252 (1991) 1-15.
22. N.P. Singh, M.T. McCoy, R.R. Tice, E.L. Schneider, A simple technique for quantification of low levels of DNA damage in individual cells, Exp. Cell Res. 175 (1988) 184-191.
23. G. Speit, A. Hartmann, The contribution of excision repair to the DNA effects seen in the alkaline single cell gel test (comet assay), Mutagenesis 10 (1995) 555-559.
24. A. Hartmann, G. Speit, Genotoxic effects of chemicals in the single cell gel (SCG) test with human blood cells in relation to the induction of sister-chromatid exchanges (SCE), Mutation Res. 346 (1995) 49-56.
25. H.H. Evans, M. Ricanati, M.-F. Horng, Q. Jiang, J. Mencl, P. Olive, DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines, Radiation Res. 134 (1993) 307-315.
26. G.M. Ross, J.J. Eady, N.P. Mithal, C. Bush, G.G. Steel, P.A. Jeggo, T.J. McMillan, DNA strand break rejoining defect in xrs-6 is complemented by transfection with the human Ku80 gene, Cancer Res. 55 (1995) 1235-1238.
27. A.J. Ward, P.L. Olive, A.H. Burr, M.P. Rosin, Response of fibroblast cultures from ataxia-telangiectasia patients to reactive oxygen species generated during inflammatory reactions, Environ. Mol. Mutagen. 24 (1994) 103-111.
28. L.F. Povirk, C.W. Houlgrave, Effect of apurinic/apyrimidinic endonucleases and polyamines on DNA treated with bleomycin and neocarzinostatin: specific formation and cleavage of closely opposed lesions in complementary strands, Biochemistry 27 (1988) 3850-3857.
29. A. Giaccia, R. Weinstein, J. Hu, T.D. Stamato, Cell cycle-dependent repair of double-strand DNA breaks in a γ-ray-sensitive Chinese hamster cell, Somat. Cell. Mol. Genet. 11 (1985) 485-491.
30. D. Frankenberg, M. Frankenberg-Schwager, D. Bloecher, R. Harbich, Evidence for DNA double-strand breaks as the critical lesions in yeast cells irradiated with sparsely or densely ionising radiation under oxic or anoxic conditions, Radiation Res. 88 (1981) 524-532.
31. J.W. Phillips, W.F. Morgan, DNA double-strand breaks in mutagenesis, Environ. Mol. Mutagen. 22 (1993) 214-217.
32. P.L. Olive, D. Wlodek, R.E. Durand, J.P. Bànath, Factors influencing DNA migration from individual cells subjected to gel electrophoresis, Exp. Cell. Res. 198 (1992) 259-267.