Exploiting viral cell-targeting abilities in a single polypeptide, non- infectious, recombinant vehicle for integrin-mediated DNA delivery and gene expression

Biotechnology and Bioengineering

Volumn 68, Issue 6, 2000, Pages 689-696

  Arís, Anna ^a   Feliu, Jordi X ^a   Knight, Andrew ^b   Coutelle, Charles ^b   Villaverde, Antonio ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Cell targeting; FMDV; Integrin; Oligolysine; Recombinant protein; RGD

Indexed keywords

AFFINITY CHROMATOGRAPHY; BIOTECHNOLOGY; CELL CULTURE; CONTROLLED DRUG DELIVERY; DNA; GENES; GENETIC ENGINEERING; MIXTURES; VIRUSES;

CELL TARGETING; DNA TRANSFER; FOOT AND MOUTH DISEASE; INTEGRIN; OLIGOLYSINE;

POLYPEPTIDES;

BETA GALACTOSIDASE; INTEGRIN; LYSINE; POLYPEPTIDE; RECOMBINANT PROTEIN;

ARTICLE; COMPLEX FORMATION; DNA PURIFICATION; DNA REPLICATION; DNA TRANSCRIPTION; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; RECEPTOR BINDING; REPORTER GENE; TARGET CELL; VIRUS CELL INTERACTION;

AMINO ACID SEQUENCE; ANIMALS; APHTHOVIRUS; BASE SEQUENCE; BETA-GALACTOSIDASE; BIOTECHNOLOGY; DNA, RECOMBINANT; DRUG DELIVERY SYSTEMS; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; LAC OPERON; LUCIFERASES; MOLECULAR SEQUENCE DATA; RECEPTORS, VITRONECTIN; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI; FOOT-AND-MOUTH DISEASE VIRUS; MAMMALIA;

EID: 0342748562     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000620)68:6<689::AID-BIT13>3.0.CO;2-A     Document Type: Article

References (31)

1. Ahmad I, Longenecker M, Samuel J, Allen TM. 1993. Antibody-targeted delivery of doxorubicin entrapped in sterically stabilized liposomes can eradicate lung cancer in mice. Cancer Res 53:1484-1488.
2. Arap W, Pasqualini R, Ruoslathi E. 1948. Cancer treatment by targeted drug delivery to tumor vasculature in a mouse model. Science 279: 377-380.
3. Benito A, Feliu JX, Villaverde A. 1995. Peptide insertions in β-galactosidase activating interface can improve binding in TPEG-Sepharose affinity. Biotechnol Tech 9:767-770.
4. Benito A, Feliu JX, Villaverde A. 1996. β-Galactosidase enzymatic activity as a molecular probe to detect specific antibodies. J Biol Chem 271:21231-21256.
5. Berinstein A, Roivainen N, Hovi T, Mason PW, Baxt B. 1995. Antibodies to the vitronectin receptor (integrin alpha V beta 3) inhibit binding and infection of foot-and-mouth disease virus to cultured cells. J Virol 69:2664-2666.
6. Brooks PC, Clark RA, Cheresh DA. 1994a. Requirement of vascular integrin alpha v beta 3 for angiogenesis. Science 264:569-571.
7. Brooks PC, Montgomery AM, Rosenfeld M, Reisfeld RA, Hu T, Klier G. 1994b. Integrin alpha v beta 3 antagonists promote tumor regression by inducing apoptosis of angiogenic blood vessels. Cell 79: 1157-1164.
8. Brooks PC, Stromblad S, Klemke R, Visscher D, Sarkar FH, Cheresh DA. 1995. Antiintegrin alpha v beta 3 blocks human breast cancer growth and angiogenesis in human skin. J Clin Invest 96:1815-1822.
9. Carbonell X, Feliu JX, Benito A, Villaverde A. 1998. Display-induced antigenic variation in recombinant peptides. Biochem Biophys Res Commun 248:773-777.
10. Feliu JX, Villaverde A. 1998. Engineering of solvent-exposed loops in Escherichia coli β-galactosidase. FEBS Lett 434:23-27.
11. Feliu JX, Benito A, Oliva B, Avilés X, Villaverde A. 1998. Conformational flexibility in a highly mobile protein loop of foot-and-mouth disease virus: Distinct structural requirements for integrin and antibody binding. J Mol Biol 283:331-338.
12. Feliu JX, Cubarsi R, Villaverde A. 1998. Optimized release of recombinant proteins by ultrasonication of E. coli cells. Biotechol Bioeng 58: 536-540.
13. Hanahan D. 1983. Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557-580.
14. Harbottle RP, Cooper RG, Hart SL, Ladhoff A, McKay T, Knight AM, Wagner E, Miller AD, Coutelle C. 1998. An RGD-oligolysine peptide: A prototype construct for integrin-mediated gene delivery. Hum Gene Ther 9:1037-1047.
15. Jackson T, Sharma A, Ghazaleh RA, Blakemore WE, Ellard FM, Simmons DL, Newman JWI, Stuart DI, King AMQ. 1997. Arginine-glycine-aspartic acid-specific binding by foot-and-mouth disease viruses to the purified integrin alpha(v)beta3 in vitro. J Virol 71:8357-8361.
16. Kalderon D, Roberts BL, Richardson WD, Smith AE. 1984. A short amino acid sequence able to specify nuclear location. Cell 39:499-509.
17. Koivunen E, Wang B, Ruoslahti E. 1994. Isolation of a highly specific ligand for the alpha 5 beta 1 integrin from a phage display library. J Cell Biol 124:373-380.
18. Maniatis D, Sambrook J, Fritsch EF. 1982. Molecular cloning: A laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
19. Mateu MG, Martínez MA, Capucci L, Andreu D, Giralt E, Brocchi E, Domingo E. 1990. A single amino acid substitution affects multiple overlapping epitopes in the major antigenic site of foot-and-mouth disease virus of serotype C. J Gen Virol 71:629-637.
20. Mateu MG, Veleron ML, Andreu D, Domingo E. 1996. Systematic replacement of amino acid residues within an Arg-Gly-Asp-containing loop of foot-and-mouth disease virus and effect on cell recognition. J Biol Chem 271:12814-12819.
21. Miller JH. 1972. In: Experiments in molecular genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. pp 352-355.
22. Neff S, Sa-Carvalho D, Rieder E, Mason PW, Blystone SD, Brown EJ, Baxt B. 1998. Foot-and-mouth disease virus virulent for cattle utilizes the integrin alpha(v)beta3 as its receptor. J Virol 72:3587-3594.
23. Pasqualini R, Koivunen E, Ruoslahti E. 1997. Alpha v integrins as receptors for tumor targeting by circulating ligands. Nature Biotech 15: 542-546
24. Pasqualini R, Koivunen E, Ruoslahti E. 1995. A peptide isolated from phage display libraries is a structural and functional mimic of an RGD-binding site on integrins. J Cell Biol 130: 1189-1196.
25. Paul RW, Weisser KE, Loomis A, Sloane DL, LaFoe D, Atkinson EM, Overell RW. 1997. Gene transfer using a novel fusion protein, GAL4/ invasin. Hum Gene Ther 8:1253-1262.
26. Pierschbacher MD, Ruoslahti E. 1984. Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule. Nature 309:30-33.
27. Pierschbacher MD, Ruoslahti E. 1987. Influence of stereochemistry of the sequence Arg-Gly-Asp-Xaa on binding specificity in cell adhesion. J Biol Chem 262:17294-17298.
28. Sosnowski BA, Gonzalez AM, Chandler LA, Buechler YJ, Pierce GF, Baird A. 1996. Targeting DNA to cells with basic fibroblast growth factor (FGF2). J Biol Chem 271:33647-33653.
29. Villaverde A, Feliu JX, Harbottle R, Benito A, Coutelle C. 1996. A recombinant, arginine-glycine-aspartic acid (RGD) motif from foot-and-mouth disease virus binds mammalian cells through vitronectin and at lower extend fibronectin receptors. Gene 180:101-106.
30. Villaverde A, Feliu JX, Aris A, Harbottle RP, Benito A, Coutelle C. 1998. A cell adhesion peptide from foot-and-mouth disease virus can direct cell targeted delivery of a functional enzyme. Biotechnol Bioeng 59: 249-301.
31. Wickham TJ, Mathias P, Nemerow GR. 1993. Integrins alpha v beta 3 and alpha v beta 5 promote adenovirus internalization but not virus attachment. Cell 73:309-319.