Molecular cloning, functional characterization and genomic organization of four alternatively spliced isoforms of the human organic cation transporter 1 (hOCT1/SLC22A1)

Annals of Human Genetics

Volumn 63, Issue 6, 1999, Pages 473-482

  Hayer, M ^a   Bonisch H ^a   Bruss M ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

1 METHYL 4 PHENYLPYRIDINIUM; CARRIER PROTEIN; CATION; COMPLEMENTARY DNA; DNA;

ALTERNATIVE RNA SPLICING; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; EXON; GENE EXPRESSION; GENE STRUCTURE; GLIOMA CELL; HUMAN; HUMAN CELL; INTRON; ION TRANSPORT; LIVER; MOLECULAR CLONING; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

1-METHYL-4-PHENYLPYRIDINIUM; ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; BASE SEQUENCE; CARRIER PROTEINS; CELL LINE; CLONING, MOLECULAR; DNA PRIMERS; DNA, COMPLEMENTARY; EXONS; GENE EXPRESSION; HUMANS; INTRONS; ION TRANSPORT; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; ORGANIC CATION TRANSPORTER 1; PROTEIN ISOFORMS; SEQUENCE HOMOLOGY, AMINO ACID; TRANSFECTION;

EID: 0342699762     PISSN: 00034800     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0003480099007770     Document Type: Article

References (31)

1. BÖNISCH, H. (1980). Extraneuronal transport of catecholamines. Pharmacology 21, 93-108.
2. BREATHNACH, R. & CHAMBON, P. (1981). Organization of eukaryotic split genes coding for protein. Annu. Rev. Biochem. 50, 349-383.
3. BREIDERT, T., SPITZENBERGER, F., GRÜNDEMANN, D. & SCHÖMIG, E. (1998). Catecholamine transport by the organic cation transporter type 1 (OCT1). Br. J. Pharmacol. 125, 218-224.
4. BURTON, L. D., KIPPENBERGER, A. G., LINGEN, B., BRÜSS, M., BÖNISCH, H. & CHRISTIE, D. L. (1998). A variant of the bovine noradrenaline transporter reveals the importance of the C-terminal region for correct targeting to the membrane and functional expression. Biochem. J. 330, 909-914.
5. BUSCH, A. E., QUESTER, S., ULZHEIMER, J. C., GORBOULEV, V., AKHOUNDOVA, A., WALDEGGER, S., LANG, F. & KOEPSELL, H. (1996). Monoamine neuro-transmitter transport mediated by the polyspecific cation transporter rOCT1. FEBS Letts 395, 153-156.
6. CHEN, C. & OKAYAMA, H. (1987). High efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7, 2745-2752.
7. DONAVAN, D. M., VANDENBERGH, D. J., PERRY, M. P., BIRD, G. S., INGERSOLL, R., NATHAKUMAR, E. & UHL, G. R. (1995). Human and mouse dopamine transporter genes: conservation of 5′-flanking sequence elements and gene structures. Mol. Brain. Res. 30, 327-335.
8. GORBOULEV, V., ULZHEIMER, J. C., AKHOUNOVA, A., ULZHEIMER-TEUBER, I., KARBACH, U., QUESTER, S., BAUMANN, C., LANG, F., BUSCH, A. E. & KOEPSELL, H. (1997). Cloning and characterization of two human polyspecific organic cation transporters. DNA Cell. Biol. 16, 871-881.
9. GROHMANN, M. & TRENDELENBURG, U. (1984). The substrate specificity of uptake2 in the rat heart. Naunyn-Schmiedeberg's Arch. Pharmacol. 328, 164-173.
10. GRÜNDEMANN, D., GORBOULEV, D., GAMBARYAN, S., VEHYL, M. & KOEPSELL, H. (1994). Drug excretion mediated by a new prototype of polyspecific transporter. Nature 372, 549-552.
11. 1 cells. J. Biol. Chem. 272, 10408-10413.
12. GRÜNDEMANN, D., SCHECHINGER, B., RAPPOLD, G. A. & SCHÖMIG, E. (1998). Molecular identification of the corticosterone-sensitive extraneuronal catecholamine transporter. Nature Neuroscience 1, 349-351.
13. HOFMANN, K. & STOFFEL, W. (1993). Tmbase - A database of membrane spanning proteins segments. Biol. Chem. Hoppe-Seyler 347, 166.
14. KEKUDA, R., PRASAD, P. D., WU, X., WANG, H., FEI, Y. J., LEIBACH, F. H. & GANAPATHY, V. (1998). Cloning and functional characterization of a potential-sensitive, polyspecific organic cation transporter (OCT3) most abundantly expressed in placenta. J. Biol. Chem. 273, 15971-15979.
15. KOEHLER, M. R., WISSINGER, B., GORBOULEV, V., KOEPSELL, H. & SCHMID, M. (1997). The human organic cation transporter genes SLC22A1 and SLC22A2 are located on chromosome 6q26. Cytogenet. Cell Genet. 79, 198-200.
16. KOEPSELL, H., BUSCH, A., GORBOULEV, V. & ARNDT, P. (1998). Structure and function of renal organic cation transporters. News Physiol. Sci. 13, 11-16.
17. LOWRY, O., ROSENBROUGH, N., FARR, A. & RANDALL, R. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
18. MACK, F. & BÖNISCH, H. (1979). Dissociation constants and lipophilicity of catecholamines and related compounds. Naunyn-Schmiedeberg's Arch. Pharmacol. 310, 1-9.
19. MEYER, J., WIEDEMANN, P., OKLADNOVA, O., BRÜSS, M., STAAB, T., STÖBER, G., RIEDERER, P., BÖNISCH, H. & LESCH, K. P. (1998). Cloning and functional characterization of the human norepinephrine transporter gene promoter. J. Neural Transm. 105, 1341-1350.
20. OKUDA, M., SAITO, H., URAKAMAI, Y., TAKANO, M. & INUI, K. (1996). cDNA cloning and functional expression of a novel rat kidney organic cation transporter. OCT2. Biochim. Biophys. Acta 224, 500-507.
21. PÖRZGEN, P., BÖNISCH, H., HAMMERMANN, R. & BRÜSS. M. (1998). The human noradrenaline transporter gene contains multiple polyadenylation sites and two alternatively spliced C-terminal exons. Biochim. Biophys. Acta 1398, 365-370.
22. SANGER, F., NICKLEN, S. & COULSON, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
23. SCHWEIFER, N. & BARLOW, D. P. (1996). The Lx1 gene maps to mouse Chromosome 17 and codes for a protein that is homologous to glucose and polyspecific transmembrane transporters. Mamm. Genome 7, 735-740.
24. 2) in human glioma cells. Naunyn-Schmiedeberg's Arch. Pharmacol. 353, 328-333.
25. TAMAI, I., YABUUCHI, H., NEZU, J., SAI, Y., OKU, A., SHIMANE, M. & TSUJI A. (1997). Cloning and characterization of a novel human pH-dependent organic cation transporter. OCTN1. FEBS Letters 419, 107-111.
26. TAMAI, I., OHASHI, R., NEZU, J., YABUUCHI, H., OKU, A., SHIMANE, M., SAI, Y. & TSUJI, A. (1998). Molecular and functional identification of sodium ion-dependent, high affinity human carnitine transporter OCTN2. J. Biol. Chem. 273, 20378-20382.
27. TERASHITA, S., DRESSER, M. J., ZHANG, L., GRAY, A. T., YOST, S. C. & GIACOMINI, K. M. (1998). Molecular cloning and functional expression of a rabbit renal organic cation transporter. Biochim. Biophys. Acta 1369, 1-6.
28. VERHAAGH, S., SCHWEIFER, N., BARLOW, D. P. & ZWART, R. (1999). Cloning of the mouse and human solute carrier 22a3 (Slc22a3/SLC22A3) identifies a conserved cluster of three organic cation transporters on mouse chromosome 17 and human 6q26-q27. Genomics 55, 209-218.
29. ZHANG, L., DRESSER, M. J., GRAY, A. T., YOST, S. C., TERASHITA, S. & GIACOMINI, K. M. (1997a). Cloning and functional expression of a human liver organic cation transporter. Mol. Pharmacol. 51, 913-921.
30. ZHANG, L., DRESSER, M. J., CHUN, J. K., BABBITT, P. C. & GIACOMINI, K. M. (1997b). Cloning and functional characterization of a rat renal organic cation transporter isoform (rOCT1A). J. Biol. Chem. 272, 16548-16554.
31. ZHANG, L., BRETT, C. M. & GIACOMINI, K. M. (1998). Role of organic cation transporters in drug absorption and elimination. Annu. Rev. Pharmacol. Toxicol. 38, 431-60.