Kinetics and metabolic specificities of Vero cells in bioreactor cultures with serum-free medium

Cytotechnology

Volumn 42, Issue 1, 2003, Pages 1-11

  Quesney, Sébastien ^a   Marc, Annie ^d   Gerdil, Catherine ^b   Gimenez, Cyrille ^b   Marvel, Jacqueline ^c   Richard, Yves ^e   Meignier, Bernard ^b  

^a BIOMÉRIEUX   (France)

^b SANOFI PASTEUR   (France)

^c INSERM   (France)

^d CNRS   (France)

^e VETAGRO SUP   (France)

Author keywords

Amino acid; Glucose; Lactate; Metabolism; Microcarriers; Serum free medium; Vero cells

Indexed keywords

AMMONIA; GLUCOSE; GLUTAMINE; LACTIC ACID; SERINE;

AMINO ACID METABOLISM; ANIMAL CELL; APOPTOSIS; ARTICLE; BIOREACTOR; CELL CULTURE; KINETICS; MICROCARRIER CULTURE; NONHUMAN; VERO CELL;

ANIMALIA;

EID: 0242642143     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026185615650     Document Type: Article

References (39)

1. Abercrombie M. and Ambrose E.J. 1962. The surface properties of cancer cells: A review. Cancer Res 22: 525-548.
2. Al-Rubeai M. 1998. Apoptosis and cell culture technology. Adv Biochem Eng Biotechnol 59: 225-249.
3. Bonarius H.P.J., Hatzimanikatis V., Meesters K.P.H., de Gooijer C.D., Schmid G. and Tramper J. 1995. Metabolic flux analysis of hybridoma cells in different culture media using mass balances. Biotechnol Bioeng 50: 299-318.
4. Clark J.M., Gebb C. and Hirtenstein M.D. 1982. Serum supplements and serum-free media: applicability for microcarrier culture of animal cells. Devel Biol Stand 50: 81-91.
5. Cruz J.H., Moreira J.L. and Carrondo M.J.T. 1999. Metabolic shifts by nutrient manipulation in continuous cultures of BHK cells. Biotechnol Bioeng 66: 104-113.
6. Doverskog M., Ljunggren J., Öhaman L. and Häggström L. 1992. Physiology of cultured animal cells. J BioTechnol 59: 103-115.
7. Duval D., Demangel C., Miossec S. and Geahel I. 1992. Role of metabolic waste products in the control of cell proliferation and antibody production by mouse hybridoma cells. Hybridoma 11: 311-322.
8. Europa A.F., Gambhir A., Fu P.G. and Hu W.S. 1999. Multiple steady states with distinct metabolism in continuous culture of mammalian cells. Biotechnol Bioeng 67: 25-34.
9. Fenge C., Klein C., Heuer C., Siegel U. and Fraune E. 1993. Agitation, aeration and perfusion modules for cell culture bioreactors. Cytotechnology 11: 233-244.
10. Glacken M.W. 1988. Catabolic control of mammalian cell culture. Biotechnol 6: 1041-1050.
11. Gstraunthaler G., Seppi T. and Pfaller W. 1999. Impact of culture conditions, culture media volume, and glucose content on metabolic properties of renal epithelial cell cultures. Cell Physiol Biochem 9: 150-172.
12. Guppy M., Greiner E. and Brand K. 1993. The role of the crabtree effect and endogenous fuel in the energy metabolism of resting and proliferating thymocytes. Eur J Biochem 212: 95-99.
13. Hassel T., Gleave S. and Butler M. 1990. Growth inhibition in animal cell culture. The effect of lactate and ammonia. Appl Biochem Biotechnol 30: 29-41.
14. Irani N., Wirth M., Van Den Heuvel J. and Wagner R. 1999. Improvement of the primary metabolism of cell cultures by introducing a new cytoplasmic pyruvate carboxylase reaction. Biotechnol Bioeng 66: 239-246.
15. Lee G.T-Y. and Engelhardt D.L. 1977. Protein metabolism during growth of Vero cells. J Cell Physiol 92: 293-302.
16. Linz M., Zeng A.P., Wagner R. and Deckwer W.D. 1997. Stoichiometry, kinetics and regulation of glucose and aminoacid metabolism of a recombinant BHK cell line in batch and continuous cultures. Biotechnol Prog 13: 453-463.
17. Mendonça R.Z., Ioshimoto L.M., Mendonça R.M.Z., De-Franco M., Valentini E.J.G., Begak W. et al. 1993. Preparation of human rabies vaccine in Vero cell culture using a microcarrier system. Braz J Med Biol Res 26: 1305-1317.
18. Mendonça R.Z. and Pereira C.A. 1997. Cell metabolism and medium perfusion in Vero cell cultures on microcarriers in a bioreactor. Bioprocess Eng 18: 213-218.
19. Merten O.W., Kierulff J.V., Castignolles N. and Perrin P. 1994. Evaluation of the new serum-free medium (MDSS2) for the production of different biologicals: Use of various cell lines. Cytotechnology 14: 47-59.
20. Montagnon J.B., Vincent-Falquet J.C. and Fanget B. 1984a. Thousand litre scale microcarrier culture of Vero cells for killed polio virus vaccine. Promising results. Devel Biol Stand 55: 37-42.
21. Montagnon J.B., Fanget B. and Vincent-Falquet J.C. 1984b. Industrial-scale production of inactivated poliovirus vaccine prepared by culture of Vero cells on microcarrier. Rev Infect Dis 6: 341-344.
22. Nahapetian A.T., Thomas J.N. and Thilly W.G. 1986. Optimization of environment for high density Vero cell culture: effect of dissolved oxygen and nutrient supply on cell growth and changes in metabolites. J Cell Sci 81: 65-103.
23. Newsholme E.A., Crabtree B. and Ardawi M.S.M. 1985. The role of high rates of glycolysis and glutamine utilization in rapidly dividing cells. Bioscience Rep 5: 393-400.
24. Nyberg G.B., Balcarcel R.R., Follstad B.D., Stephanopoulos G. and Wang D.I.C. 1999. Metabolism of peptide amnoacids by Chinese Hamster Ovary cells grown in a complex medium. Biotechnol Bioeng 62: 324-335.
25. Oller A.R., Buser C.W., Tyo M.A. and Thilly W.G. 1989. Growth of mammalian cells at high oxygen concentrations. J Cell Sci 94: 43-49.
26. Ozturk S.S., Jorjani P., Taticek R., Lowe B., Shackleford S., Ladehoff-Guiles D. et al. 1997. Kinetics of glucose metabolism and utilization of lactate in mammalian cell cultures. In: Carrondo M.J.T. et al. (eds), Animal Cell Technology. Kluwer Academic Publishers, Netherlands, pp. 355-360.
27. Papaconstantinou H.T., Hwang K.O., Rajaraman S., Hellmich M.R., Townsend C.M. and Ko T.C. 1998. Glutamine deprivation induces apoptosis in intestinal epithelial cells. Surgery 124: 152-160.
28. Pardridge W.M. and Casanello-Ertl D. 1979. Effects of glutamine deprivation on glucose and amino acid metabolism in tissue culture. Am J Physiol 236: 234-238.
29. Philips H.J. 1973. In: Kruse P.F. and Patterson M.K. (eds), Tissue cultures: Methods and Applications. Academic Press, New York, pp. 406-408.
30. Polastri G.D., Friensen H.J. and Mauler R. 1982. Aminoacid utilisation by Vero cells in microcarrier culture. Devel Biol Stand 55: 53-56.
31. Quesney S., Marvel J., Marc A., Gerdil C. and Meignier B. 2001. Characterization of Vero cell growth and death in bioreactor with serum-containing and serum-free media. Cytotechnology 35: 115-125.
32. Sakagami H., Satoh M., Yokote Y., Takano H., Takahama M., Kochi M. et al. 1998. Amino acid utilization during cell growth and apoptosis induction. Anticancer Res 18: 4303-4306.
33. Sandford K.K., Earle W.R., Evans V.J., Waltz H. and Shannon J.E. 1951. The measurement of proliferation in tissue cultures by enumeration of cell nuclei. J Natl Cancer Inst 11: 773-795.
34. Sanfeliu A., Paredes C., Cairo J.J. and Godia F. 1997. Analysis of glucose and glutamine metabolism of hybridoma cells by continuous culture experiments. In: Carrondo M.J.T. et al. (eds), Animal Cell Technology. Kluwer Academic Publishers, Netherlands, pp. 785-789.
35. Van Wezel A.L. 1973. Microcarrier cultures of animals cells. In: Kruse P.F. and Patterson M.K. (eds), Tissue cultures: Methods and Applications. Academic Press, New York, pp. 372-377.
36. Wagner A., Marc A., Engasser J.M. and Einsele A. 1991. Growth and metabolism of human tumor kidney cells on galactose and glucose. Cytotechnology 7: 7-13.
37. Yasumura Y. and Kawakita Y. 1963. Studies on SV40 in tissue culture - Preliminary step for cancer research in vitro. Nihon Rinsho (in Japanese) 21: 1201-1221.
38. Zielke H.R., Zielke C.L. and Ozand P.T. 1984. Glutamine: a major energy source for cultured mammalian cells. Fed Proc 43: 121-125.
39. Zielke H.R., Sumbilla C.M. and Sevdalain D.A. 1980. Lactate: a major product of glutamine metabolism by human diploid fibroblasts. J Cell Physiol 104: 433-441.