MgrA, an Orthologue of Mga, Acts as a Transcriptional Repressor of the Genes within the rlrA Pathogenicity Islet in Streptococcus pneumoniae

Journal of Bacteriology

Volumn 185, Issue 22, 2003, Pages 6640-6647

  Hemsley, Carolyn ^a   Joyce, Elizabeth ^b   Hava, David L ^c   Kawale, Amita ^b   Camilli, Andrew ^c  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL INFECTION; BACTERIAL VIRULENCE; BACTERIUM ADHERENCE; GENE EXPRESSION REGULATION; GENE REPRESSION; GENETIC TRANSCRIPTION; MGRA GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESPIRATORY TRACT INFECTION; RLRA GENE; STREPTOCOCCUS PNEUMONIAE;

ANIMALS; BACTERIAL PROTEINS; FEMALE; GENE EXPRESSION REGULATION, BACTERIAL; HUMANS; MICE; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PNEUMONIA, PNEUMOCOCCAL; REPRESSOR PROTEINS; RIBONUCLEASES; STREPTOCOCCUS PNEUMONIAE; TRANS-ACTIVATORS; TRANSCRIPTION, GENETIC; VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); MURINAE; POSIBACTERIA; STREPTOCOCCUS; STREPTOCOCCUS PNEUMONIAE; STREPTOCOCCUS SP. 'GROUP A';

EID: 0242575013     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.22.6640-6647.2003     Document Type: Article

References (30)

1. Acebo, P., C. Nieto, M. A. Corrales, M. Espinosa, and P. Lopez. 2000. Quantitative detection of Streptococcus pneumoniae cells harbouring single or multiple copies of the gene encoding the green fluorescent protein. Microbiology 146:1267-1273.
2. Akerley, B. J., P. A. Cotter, and J. F. Miller. 1995. Ectopic expression of the flagellar regulon alters development of the Bordetella-host interaction. Cell 80:611-620.
3. Akerley, B. J., E. J. Rubin, A. Camilli, D. J. Lampe, H. M. Robertson, and J. J. Mekalanos. 1998. Systematic identification of essential genes by in vitro mariner mutagenesis. Proc. Natl. Acad. Sci. USA 95:8927-8932.
4. Bricker, A. L., and A. Camilli. 1999. Transformation of a type 4 encapsulated strain of Streptococcus pneumoniae. FEMS Microbiol. Lett. 172:131-135.
5. Eisen, M. B., P. T. Spellman, P. O. Brown, and D. Botstein. 1998. Cluster analysis and display of genome-wide expression patterns. Proc. Natl. Acad. Sci. USA 95:14863-14868.
6. Gollub, J., C. A. Ball, G. Binkley, J. Demeter, D. B. Finkelstein, J. M. Herbert, T. Hernandez-Boussard, H. Jin, M. Kaloper, J. C. Maltese, M. Schroeder, P. O. Brown, D. Botstein, and G. Sherlock. 2003. The Standford Microarray Database: data access and quality assessment tools. Nucleic Acids Res. 31:94-96.
7. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
8. Hava, D. L., and A. Camilli. 2002. Large-scale identification of serotype 4 Streptococcus pneumoniae virulence factors. Mol. Microbiol. 45:1389-1405.
9. Hava, D. L., C. J. Hemsley, and A. Camilli. 2003. Transcriptional regulation in the Streptococcuspneumoniae rlrA pathogenicity islet by RlrA. J. Bacteriol. 185:413-421.
10. Kaper, J. B., and J. Hacker. 2000. Pathogenicity islands and the evolution of microbes. Annu. Rev. Microbiol. 54:641-679.
11. Kolter, R., M. Inuzuka, and D. R. Helinski. 1978. Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K. Cell 15:1199-1208.
12. Lange, R., C. Wagner, A. de Saizieu, N. Flint, J. Molnos, M. Stieger, P. Caspers, M. Kamber, W. Keck, and K. E. Amrein. 1999. Domain organization and molecular characterization of 13 two-component systems identified by genome sequencing of Streptococcus pneumoniae. Gene 237:223-234.
13. Lau, G. W., S. Haataja, M. Lonetto, S. E. Kensit, A. Marra, A. P. Bryant, D. McDevitt, D. A. Morrison, and D. W. Holden. 2001. A functional genomic analysis of type 3 Streptococcus pneumoniae virulence. Mol. Microbiol. 40:555-571.
14. Leowiski, J. 1986. Mortality from acute respiratory infections in children under 5 years of age: global estimates. World Health Stat. 39:138-144.
15. Martin, B., M. Prudhomme, G. Alloing, C. Granadel, and J. P. Claverys. 2000. Cross-regulation of competence pheromone production and export in the early control of transformation in Streptococcus pneumoniae. Mol. Microbiol. 38:867-878.
16. Mascher, T., D. Zahner, M. Merai, N. Balmelle, A. B. De Saizieu, and R. Hakenbeck. 2003. The Streptococcus pneumoniae cia regulon: CiaR target sites and transcription profile analysis. J. Bacteriol. 185:60-70.
17. McIver, K., and R. L. Myles. 2002. Two DNA-binding domains of Mga are required for virulence gene activation in the group A streptococcus. Mol. Microbiol. 43:1591-1601.
18. Musher, D. M. 1992. Infections caused by Streptococcuspneumoniae: clinical spectrum, pathogenesis and treatment. Clin. Infect. Dis. 14:801-809.
19. Peterson, S., R. T. Cline, H. Tettelin, V. Sharov, and D. A. Morrison. 2000. Gene expression analysis of the Streptococcuspneumoniae competence regulons by use of DNA microarrays. J. Bacteriol. 182:6192-6202.
20. Pobielski, A., M. Woischnik, B. A. Leonard, and K. H. Schmidt. 1999. Characterisation of nra, a global negative regulator gene in group A streptoeocei. Mol. Microbiol. 31:1051-1064.
21. Pobielski, A., M. Woischnik, B. Pohl, and K. H. Schmidt. 1996. What is the size of the group A streptococcal vir regulon? The Mga regulator affects expression of secreted and surface virulence factors. Med. Microbiol. Immunol. 185:171-181.
22. Polissi, A., A. Pontiggia, G. Feger, M. Altieri, H. Motti, L. Ferrari, and D. Simon. 1998. Large-scale identification of virulence genes from Streptococcus pneumoniae. Infect. Immun. 66:5620-5629.
23. Puyet, A., and M. Espinosa. 1993. Structure of the maltodextrin-uptake locus of Streptococcus pneumoniae. Correlation to the Escherichia coli maltose regulon. J. Mol. Biol. 230:800-811.
24. Rasmussen, M., A. Edén, and L. Björck. 2000. SclA, a novel collagen-like surface protein of Streptococcus pyogenes. Infect. Immun. 68:6370-6377.
25. Schuchat, A., K. Robinson, and J. D. Wenger. 1997. Bacterial meningitis in the United States in 1995. N. Engl. J. Med. 337:970-976.
26. Sebert, M. E., L. M. Palmer, M. Rosenberg, and J. N. Weiser. 2002. Microarray-based identification of htrA, a Streptococcus pneumoniae gene that is regulated by the CiaRH two-component system and contributes to naso-pharyngeal colonization. Infect. Immun. 70:4059-4067.
27. Sherlock, G., T. Hernandez-Boussard, A. Kasarskis, G. Binkley, J. C. Matese, S. S. Dwight, M. Kaloper, S. Weng, H. Jin, C. A. Ball, M. B. Eisen, P. T. Spellman, P. O. Brown, D. Botstein, and J. M. Cherry. 2001. The Stanford Microarray Database. Nucleic Acids Res. 29:152-155.
28. Terao, Y., S. Kawabata, E. Kunitomo, J. Murakami, I. Nakagawa, and S. Hamada. 2001. Fba, a novel fibronectin-binding protien from Streptococcus pyogenes, promotes bacterial entry into epithelial cells, and the fba gene is positively transcribed under the Mga regulator. Mol. Microbiol. 42:75-86.
29. Throup, J. P., K. K. Koretke, A. P. Bryant, K. A. Ingraham, A. F. Chalker, Y. Ge, A. Marra, N. G. Wallis, J. R. Brown, D. J. Holmes, M. Rosenberg, and M. K. Burnham. 2000. A genomic analysis of two-component signal transduction in Streptococcus pneumoniae. Mol. Microbiol. 35:566-576.
30. Tusher, V. G., R. Tibshirani, and G. Chu. 2001. Significance analysis of microarrays applied to the ionizing radiation response. Proc. Natl. Acad. Sci. USA 98:5116-5121.