Characterization of nra, a global negative regulator gene in group A streptococci

Molecular Microbiology

Volumn 31, Issue 4, 1999, Pages 1051-1064

  Podbielski, Andreas ^a   Woischnik, Markus ^a   Leonard, Bettina A B ^a,c   Schmidt, Karl Hermann ^b  

^a UNIVERSITY HOSPITAL ULM   (Germany)

^b FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^c TEMPLE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; COLLAGEN; FIBRONECTIN;

AMINO ACID SEQUENCE; ARTICLE; DNA HYBRIDIZATION; GENE EXPRESSION; GENE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REGULATOR GENE; SEQUENCE HOMOLOGY; SEROTYPE; STREPTOCOCCUS GROUP A;

ADHESINS, BACTERIAL; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CARRIER PROTEINS; COLLAGEN; DOWN-REGULATION; FIBRONECTINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENES, REGULATOR; INTEGRINS; MOLECULAR SEQUENCE DATA; RECEPTORS, COLLAGEN; STREPTOCOCCUS PYOGENES; TRANS-ACTIVATORS; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

ANIMALIA; POSIBACTERIA; STREPTOCOCCUS; STREPTOCOCCUS GROUP A; STREPTOCOCCUS SP. 'GROUP A';

EID: 0033039873     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1999.01241.x     Document Type: Article

References (53)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., et al. (1990) Current Protocols in Molecular Biology. New York: Greene Publishing Associates and Wiley-Interscience.
2. Brakhage, A.A., Wozny, M., and Putzer, H. (1990) Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes. Biochimie 72: 725-734.
3. Caparon, M.G., and Scott, J.R. (1991) Genetic manipulation of pathogenic streptococci. Methods Enzymol 204: 556-586.
4. Caparon, M.G., Geist, R.T., Perez-Casal, J., and Scott, J.R. (1992) Environmental regulation of virulence in group A streptococci: transcription of the gene encoding M protein is stimulated by carbon dioxide. J Bacteriol 174: 5693-5701.
5. Chen, D., and Swenson, R.P. (1994) Cloning, sequence analysis, and expression of the genes encoding the two sub-units of the methylotrophic bacterium W3A1 electron transfer flavoprotein. J Biol Chem 269: 32120-32130.
6. Chen, C., Bormann, N., and Cleary, P.P. (1993) VirR and Mry are homologous trans-acting regulators of M protein and C5a peptidase expression in group A streptococci. Mol Gen Genet 241: 685-693.
7. Crater, D.L., and van de Rijn, I. (1995) Hyaluronic acid synthesis operon (has) expression in group A streptococci. J Biol Chem 270: 18452-18458.
8. Cregg, K.M., Wilding, E.I., and Black, M.T. (1996) Molecular cloning and expression of the spsB gene encoding an essential type I signal peptidase from Staphylococcus aureus. J Bacteriol 178: 5712-5718.
9. Fogg, G.C., and Caparon, M.G. (1997) Constitutive expression of fibronectin-binding in Streptococcus pyogenes as a result of anaerobic activation of rofA. J Bacteriol 179: 6172-6180.
10. Fogg, G.C., Gibson, C.M., and Caparon, M.G. (1994) The identification of rofA, a positive-acting regulatory component of prtF expression: use of an mgd-based shuttle mutagenesis strategy in Streptococcus pyogenes. Mol Microbiol 11: 671-684.
11. Geelen, S., Bhattacharyya, C., and Tuomanen, E. (1993) Pneumococcal cell wall mediates pneumococcal attachment and cytopathology to human endothelial cells. Infect Immun 61: 1538-1543.
12. Gibson, C.M., and Caparon, M.G. (1996) Insertional inactivation of Streptococcus pyogenes sod suggests that prtF is regulated in response to a superoxide signal. J Bacteriol 178: 4688-4695.
13. Gräfe, S., Ellinger, T., and Malke, H. (1996) Structural dissection and functional analysis of the complex promoter of the streptokinase gene from Streptococus equisimilis H46A. Med Microbiol Immunol 185: 11-17.
14. Gubbe, K. (1997) Genetische und biochemische Charakterisierung von Epitopen auf Pathogenitätsfaktoren der Gruppe-A-Streptokokken. PhD Thesis, Friedrich-Schiller-Universität, Jena.
15. Hanski, E., and Caparon, M. (1992) Protein F, a fibronectin-binding protein, is an adhesin of the group A streptococcus (Streptococcus pyogenes). Proc Natl Acad Sci USA 89: 6172-6176.
16. Hanski, E., Horwitz, P.A., and Caparon, M.G. (1992) Expression of protein F, the fibronectin-binding protein of Streptococcus pyogenes JRS4, in heterologous streptococcal and enterococcal strains promotes their adherence to respiratory epithelial cells. Infect Immun 60: 5119-5125.
17. Jaffe, J., Natanson-Yaron, S., Caparon, M.G., and Hanski, E. (1996) Protein F2, a novel fibronectin-binding protein from Streptococcus pyogenes, possesses two binding domains. Mol Microbiol 21: 373-384.
18. Jenkinson, H.F., and Demuth, D.R. (1997) Structure, function and immunogenicity of streptococcal antigen I/II polypeptides. Mol Microbiol 23: 183-190.
19. Kaufhold, A., Podbielski, A., Johnson, D.R., Kaplan, E.L., and Lütticken, R. (1992) M protein gene typing of Streptococcus pyogenes by nonradioactively labeled oligonucleotide probes. J Clin Microbiol 30: 2391-2397.
20. Kleerebezem, M., Quadri, L.E.N., Kuipers, O.P., and de Vos, W.M. (1997) Quorum sensing by peptide pheromones and two-component signal-transduction systems in Gram-positive bacteria. Mol Microbiol 24: 895-904.
21. La Penta, D., Zhang, X.P., and Cleary, P.P. (1994) Streptococcus pyogenes type IIa IgG Fc receptor expression is co-ordinately regulated with M protein and streptococcal C5a peptidase. Mol Microbiol 12: 873-879.
22. McIver, K., and Scott, J.R. (1997) Role of mga in growth phase regulation of virulence genes of the group A streptococcus. J Bacteriol 179: 5178-5187.
23. McIver, K.S., Heath, A.S., and Scott, J.R. (1995) Regulation of virulence by environmental signals in group A streptococci: influence of osmolarity, temperature, gas exchange, and iron limitation in emm transcription. Infect Immun 63: 4540-4542.
24. Ma, G.T., Hong, Y.S., and Ives, D.H. (1995) Cloning and expression of the heterodimeric deoxyguanosine kinase/deoxyadenosine kinase of Lactobacillus acidophilus R-26. J Biol Chem 270: 6595-6601.
25. Machado, H.B., Yates, M.G., Funayama, S., Rigo, L.U., Steffens, M.B.R., Souza, E.M., et al. (1995) The ntrBC genes of Azospirillum brasilense are part of a nifR3-like-ntrB-ntrC operon and are negatively regulated. Can J Microbiol 41: 674-684.
26. Martin, N.J., Kaplan, E.L., Gerber, M.A., Menegus, M.A., Randolph, M., Bell, K., et al. (1990) Comparison of epidemic and endemic group G streptococci by restriction enzyme analysis. J Clin Microbiol 28: 1881-1886.
27. Natanson, S., Sela, S., Moses, A.E., Musser, J.M., Caparon, M.G., and Hanski, E. (1995) Distribution of fibronectin-binding proteins among group A streptococci of different M types. J Infect Dis 171: 871-878.
28. Ogasawara, N., Nakai, S., and Yoshikawa, H. (1994) Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin. DNA Res 1: 1-14.
29. Okada, N., Geist, R.T., and Caparon, M.G. (1993) Positive transcriptional control of mry regulates virulence in the group A streptococcus. Mol Microbiol 7: 893-903.
30. Okada, N., Pentland, A.P., Falk, P., and Caparon, M.G. (1994) M protein and protein F act as important determinants of cell-specific tropism of streptococcus pyogenes in skin tissue. J Clin Invest 94: 965-977.
31. Okada, N., Liszewski, M.K., Atkinson, J.P., and Caparon, M. (1995) Membrane cofactor protein (CD46) is a keratinocyte receptor for the M protein of the group A streptococcus. Proc Natl Acad Sci USA 92: 2489-2493.
32. Ozeri, V., Tovi, A., Burstein, I., Natanson-Yaron, S., Caparon, M.G., Yamada, K.M., et al. (1996) A two-domain mechanism for group A streptococcal adherence through protein F to the extracellular matrix. EMBO J 15: 989-998.
33. Perez-Casal, J., Caparon, M.G., and Scott, J.R. (1991) Mry, a trans-acting positive regulator of the M protein gene of Streptococcus pyogenes with similarity to the receptor proteins of two-component regulatory systems. J Bacteriol 173: 2617-2624.
34. Podbielski, A., and Leonard, B.A.B. (1998) The group A streptococcal dipeptide permease (Dpp) is involved in the uptake of essential amino acids and affects the expression of cysteine protease. Mol Microbiol 28: 1323-1334.
35. Podbielski, A., Peterson, J.A., and Cleary, P. (1992) Surface protein-CAT reporter fusions demonstrate differential gene expression in the vir regulon of Streptococcus pyogenes. Mol Microbiol 6: 2253-2265.
36. Podbielski, A., Flosdorff, A., and Weber-Heynemann, J. (1995) The group A streptococcal virR49 gene controls expression of four structural vir regulon genes. Infect Immun 63: 9-20.
37. Podbielski, A., Woischnik, M., Pohl, B., and Schmidt, K.H. (1996a) What is the size of the group A streptococcal vir regulon? The Mga regulator affects expression of secreted and surface virulence factors. Med Microbiol Immunol 185: 171-181.
38. Podbielski, A., Pohl, B., Woischnik, M., Körner, C., Schmidt, K.H., Rozdzinski, E., et al. (1996b) Molecular characterization of group A streptococcal (GAS) oligopeptide permease (Opp) and its effect on cysteine protease production. Mol Microbiol 21: 1087-1099.
39. Podbielski, A., Spellerberg, B., Woischnik, M., Pohl, B., and Lütticken, R. (1996c) Novel series of plasmid vectors for gene inactivation and expression analysis in group A streptococci (GAS). Gene 177: 137-147.
40. Prag, G., Greenberg, S., and Oppenheim, A.B. (1997) Structural principles of prokaryotic gene regulatory proteins and the evolution of repressors and gene activators. Mol Microbiol 26: 619-620.
41. Van de Rijn, I., and Kessler, R.E. (1980) Growth characteristics of group A streptococci in a new chemically defined medium. Infect Immun 27: 444-448.
42. Rikke, B.A., Vellanoweth, R.L., Her, S., Chatterjee, B., and Roy, A.K. (1995) Cloning and sequence analysis of a novel member of the single-stranded DNA binding protein family. Biochim Biophys Acta 1261: 143-146.
43. Roe, B.A., Clifton, S., McShan, M., and Ferretti, J.J. (1997) Streptococcal Genome Sequencing Project. Oklahoma City: University of Oklahoma.
44. Russell, R.R.B., Aduse-Opoku, J., Sutcliffe, I.C., Tao, L., and Ferretti, J.J. (1992) A binding protein-dependent transport system in Streptococcus mutans responsible for multiple sugar metabolism. J Biol Chem 267: 4631-4637.
45. Schmidt, K.H., Mann, K.H., Cooney, J., and Köhler, W. (1993) Multiple binding of type 3 streptococcal M protein to human fibrinogen, albumin and fibronectin. FEMS Immunol Med Microbiol 7: 135-144.
46. Sela, S., Aviv, A., Tovi, A., Burstein, I., Caparon, M.G., and Hanski, E. (1993) Protein F: an adhesin of Streptococcus pyogenes binds fibronectin via two distinct domains. Mol Microbiol 10: 1049-1055.
47. Shaw, J.H., and Clewell, D.B. (1985) Complete nucleotide sequence of macrolide-lincosamide-streptogramin B-resistance transposon Tn917 in Streptococcus faecalis. J Bacteriol 164: 782-796.
48. Takahashi, I., Okahashi, N., and Hamada, S. (1993) Molecular characterization of a negative regulator of Streptococcus sobrinus surface protein antigen gene. J Bacteriol 175: 4345-4353.
49. Talay, S.R., Valentin-Weigand, P., Jerlström, P.G., Timmis, K.N., and Chhatwal, G.S. (1992) Fibronectin-binding protein of Streptococcus pyogenes: sequence of the binding domain involved in adherence of streptococci to epithelial cells. Infect Immun 60: 3837-3844.
50. Talay, S.R., Valentin-Weigand, P., Timmis, K.N., and Chhatwal, G.S. (1994) Domain structure and conserved epitopes of Sfb protein, the fibronectin-binding adhesin of streptococcus pyogenes. Mol Microbiol 13:531-539.
51. Tijssen, P. (1985) Practice and theory of enzyme immuno-assays. In Laboratory Techniques in Biochemistry and Molecular Biology. Burdon, R.H., and Van Knippenberg, R.H. (eds). Amsterdam: Elsevier.
52. Van Heyningen, T., Fogg, G., Yates, D., Hanski, E., and Caparon, M. (1993) Adherence and fibronectin binding are environmentally regulated in the group A streptococci. Mol Microbiol 9: 1213-1222.
53. Xu, S., and Collins, C.M. (1996) Temperature regulation of the streptococcal pyrogenic exotoxin A-encoding gene (speA). Infect Immun 64: 5399-5402.