Manipulation of in vitro toxicant sensors in an ultrasonic standing wave

Toxicology in Vitro

Volumn 18, Issue 1, 2004, Pages 115-120

  Morgan, J ^a   Spengler, J F ^a,c   Kuznetsova, L ^a   Coakley, W T ^a   Xu, J ^b   Purcell, W M ^b  

^a CARDIFF UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF THE WEST OF ENGLAND   (United Kingdom)

^c Gelsenwasser AG   (United Kingdom)

Author keywords

Acoustic microstreaming; Cell trap; HepG2 spheroids; Ultrasonic standing wave trap

Indexed keywords

LATEX; TOXIC SUBSTANCE;

ARTICLE; CELL DAMAGE; CELL STRAIN HEPG2; CONTROLLED STUDY; CYTOTOXICITY; FLOW RATE; HUMAN; HUMAN CELL; IN VITRO STUDY; PRESSURE; SAMPLING; SCALE UP; SENSOR; STANDARDIZATION; TOXICITY TESTING; ULTRASOUND; WAVEFORM;

EID: 0242489034     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2003.08.004     Document Type: Article

References (19)

1. 1H NMR spectroscopy. Effects of D-galactosamine toxicity Chemical Research in Toxicology. 15:2002;1351-1359.
2. Christensen K.T., Soloff S.M., Adrian R.J. Integrated Particle Image Velocimetry Interrogation/Validation Software. Theoretical and Applied Mechanics Report No. 943. 2000;University of Illinois at Urbana, Champaign.
3. Dilworth C., Hamilton G.A., George E., Timbrell J.A. The use of liver spheroids as an in vitro model for studying induction of the stress response as a marker of chemical toxicity. Toxicology in Vitro. 14:2000;169-176.
4. Groschl M. Ultrasonic separation of suspended particles - Part I. Fundamentals Acustica. 84:1998;432-447.
5. Hansen L.K., Hisao C.-C., Friend J.R., Wu F.J., Bridge G.A., Remmel R.P., Cerra F.B., Hu W.-S. Enhanced morphology and function in hepatocyte spheroids: a model of tissue self-assembly. Tissue Engineering. 4:1998;65-74.
6. Kawahara N., Yarin A.L., Brenn G., Kastner O., Durst F. Effect of acoustic streaming on the mass transfer from a sublimating sphere. Physics of Fluids. 12:2000;912-923.
7. Khanna S., Amso N.N., Paynter S.J., Coakley W.T. Contrast agent bubble and erythrocyte behavior in a 1.5 MHz standing ultrasound wave. Ultrasound in Medicine and Biology. 2003;. (in press).
8. Landry J., Bernier D., Quellet C. In vitro formation of tissue-like structures and long-term maintenance of liver-cell functional-activity in spheroid culture. Strahlentherapie. 160:1984;60-61.
9. Lazar A., Mann H.J., Remmel R.P., Shatford R.A., Cerra F.B., Hu W.S. Extended liver-specific functions of porcine hepatocyte spheroids entrapped in collagen gel. In Vitro Cellular and Developmental Biology-Animal. 31:1995;340-346.
10. Riley N. Acoustic streaming. Theoretical and Computational Fluid Dynamics. 10:1998;349.
11. Spengler, J.F., Coakley, W.T., Christensen, K.T., 2003. Micro-streaming effects on particle concentration in an ultrasonic standing wave. AlChE Journal. (in press).
12. Spengler J.F., Jekel M., Christensen K.T., Adrian R.J., Hawkes J.J., Coakley W.T. Observation of yeast cell movement and aggregation in a small-scale MHz-ultrasonic standing wave field. Bioseparation. 9:2001;329-341.
13. Tzanakakis E.S., Hansen L.K., Hu W.S. The role of actin filaments and microtubles in hepatocyte spheroid self-assembly. Cell Motility and the Cytoskeleton. 48:2001;175-189.
14. Walker T.M., Rhodes P.C., Westmoreland C. The differential cytotoxicity of methotrexate in rat hepatocyte monolayer and spheroid cultures. Toxicology In Vitro. 14:2000;475-485.
15. Weiser M.A.H., Apfel R.E. Extension of acoustic levitation to include the study of micron-size particles in a more compressible host fluid. Journal of the Acoustical Society of America. 71:1982;1261-1268.
16. Xu J., Ma M., Purcell W.M. Characterisation of some cytotoxic endpoints using rat liver and HepG2 spheroids as in vitro models and their application in hepatotoxicity studies. I. Glucose metabolism and enzyme release as cytotoxic markers Toxicology and Applied Pharmacology. 189:2003;100-111.
17. Xu J., Ma M., Purcell W.M. Characterisation of some cytotoxic endpoints using rat liver and HepG2 spheroids as in vitro models and their application in hepatotoxicity studies. II. Spheroid cell spreading inhibition as a new cytotoxic marker Toxicology and Applied Pharmacology. 189:2003;112-119.
18. Yamada K., Kamihira M., Iijima S. Self-organization of liver constitutive cells mediated by artificial matrix and improvement of liver functions in long-term culture. Biochemical Engineering Journal. 8:2001;135-143.
19. Yumoto A.U., Watanabe S., Hirose M., Kitamura T., Yamaguchi Y., Sato N. Structural and functional features of bile canaliculi adult rat hepatocyte spheroids. Liver. 16:1996;61-66.