A Novel Step in β-Tubulin Folding Is Important for Heterodimer Formation in Saccharomyces cerevisiae

Genetics

Volumn 165, Issue 2, 2003, Pages 531-541

  Lacefield, Soni ^a   Solomon, Frank ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA TUBULIN; BETA TUBULIN; CELL PROTEIN; CHAPERONIN; DIMER; GALACTOSE; PHOSDUCIN; PROTEIN PLP1P; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DIMERIZATION; DNA SEQUENCE; FUNGAL GENE; GEL FILTRATION CHROMATOGRAPHY; IMMUNOBLOTTING; MICROTUBULE; MORPHOGENESIS; NONHUMAN; PLP1 GENE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; SACCHAROMYCES CEREVISIAE;

CARRIER PROTEINS; DIMERIZATION; GENE DELETION; GROEL PROTEIN; LIPOPROTEINS; MEMBRANE PROTEINS; MICROTUBULE-ASSOCIATED PROTEINS; PROTEIN FOLDING; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TUBULIN; UBIQUITIN-PROTEIN LIGASES;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0242286609     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (38)

1. ABRUZZI, K. C., A. SMITH, W. CHEN and F. SOLOMON, 2002 Protection from free β-tubulin by the β-tubulin binding protein Rbl2p. Mol. Cell. Biol. 22: 138-147.
2. ALANI, E., L. CAO and N. KLECKNER, 1987 A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116: 541-545.
3. ALVAREZ, P., A. SMITH, J. FLEMING and F. SOLOMON, 1998 Modulation of tubulin polypeptide ratios by the yeast protein Pac10p. Genetics 149: 857-864.
4. ARCHER, J. E., L. R. VEGA and F. SOLOMON, 1995 Rbl2p, a yeast protein that binds to β-tubulin and participates in microtubule function in vivo. Cell 82: 425-434.
5. 1 cyclin turnover and nutrient uptake are controlled by a common pathway in yeast. Genes Dev. 9: 399-409.
6. BOND, J. F., J. L. FRIDOVICH-KEIL, L. PILLUS, R. C. MULLIGAN and F. SOLOMON, 1986 A chicken-yeast chimeric β-tubulin protein is incorporated into mouse microtubules in vivo. Cell 44: 461-468.
7. BURKE, D., P. GASDASKA and L. HARTWELL, 1989 Dominant effects of tubulin overexpression in Saccharomyces cerevisiae. Mol. Cell. Biol. 9: 1049-1059.
8. BURNS, N., B. GRIMWADE, P. B. ROSS-MACDONALD, E. Y. CHOI, K. FINBERG et al., 1994 Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae. Genes Dev. 8: 1087-1105.
9. DESAI, A., and T. J. MITCHISON, 1997 Microtubule polymerization dynamics. Annu. Rev. Cell Dev. Biol. 13: 83-117.
10. DUNN, A. Y., M. W. MELVILLE and J. FRYDMAN, 2001 Review: cellular substrates of the eukaryotic chaperonin TriC/CCT. J. Struct. Biol. 135: 176-184.
11. ERICKSON, H. P., 1995 FtsZ, a prokaryotic homolog of tubulin? Cell 80: 367-370.
12. FARR, G. W., E. C. SCHARL, R. J. SCHUMACHER, S. SONDEK and A. L. HORWICH, 1997 Chaperonin-mediated folding in the eukaryotic cytosol proceeds through rounds of release of native and normative forms. Cell 89: 927-937.
13. FLANARY, P. L., P. R. DIBELLO, P. ESTRADA and H. G. DOHLMAN, 2000 Functional analysis of Plp1 and Plp2, two homologues of phosducin in yeast. J. Biol. Chem. 275: 18462-18469.
14. FLEMING, J., L. VEGA and F. SOLOMON, 2000 Function of tubulin binding proteins in vivo. Genetics 156: 69-80.
15. FLOOR, E., 1970 Interaction of morphogenetic genes of bacteriophage T4. J. Mol. Biol. 47: 293-306.
16. GEISER, J. R., E. J. SCHOTT, T. J. KINGSBURY, N. B. COLE, L. J. TOTIS et al., 1997 Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways. Mol. Biol. Cell 8: 1035-1050.
17. GEISSLER, S., K. SIEGERS and E. SCHIEBEL, 1998 A novel protein complex promoting formation of functional alpha- and gamma-tubulin. EMBO J. 17: 952-966.
18. GUTHRIE, C., and G. FINK, 1991 Guide to Yeast Genetics and Molecular Biology. Academic Press, New York.
19. HANSEN, W. J., N. J. COWAN and W. J. WELCH, 1999 Prefoldin-nascent chain complexes in the folding of cytoskeletal proteins. J. Cell Biol. 145: 265-277.
20. HOYT, M. A., J. P. MACKE, B. T. ROBERTS and J. R. GEISER, 1997 Saccharomyces cerevisiae PAC2 functions with CIN1, 2 and 4 in a pathway leading to normal microtubule stability. Genetics 146: 849-857.
21. JAVERZAT, J., G. CRANSTON and R. C. ALLSHIRE, 1996 Fission yeast genes which disrupt mitotic chromosome segregation when overexpressed. Nucleic Acids Res. 24: 4676-4683.
22. KATZ, W., B. WEINSTEIN and F. SOLOMON, 1990 Regulation of tubulin levels and microtubule assembly in Saccharomyces cerevisiae consequences of altered tubulin gene copy number in yeast. Mol. Cell. Biol. 10: 2730-2736.
23. LONGTINE, M. S., A. MCKENZIE, D. J. DEMARINI, N. G. SHAH, A. WACH et al., 1998 Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14: 953-961.
24. MCCALLUM, C. D., H. DO, A. E. JOHNSON and J. FRYDMAN, 2000 The interaction of the chaperonin tailless complex polypeptide 1 (TCP1) ring complex (TRiC) with ribosome-bound nascent chains examined using photo-cross-linking. J. Cell Biol. 149: 591-601.
25. MCLAUGHLIN, J. N., C. D. THULIN, S. J. HART, K. A. RESING, N. G. AHN et al., 2002 Regulatory interaction of phosducin-like protein with the cytosolic chaperonin complex. Proc. Natl. Acad. Sci. USA 99: 117-121.
26. SCHATZ, P. J., F. SOLOMON and D. BOTSTEIN, 1986 Genetically essential and nonessential α-tubulin genes specify functionally interchangeable proteins. Mol. Cell. Biol. 6: 3722-3733.
27. SCHATZ, P. J., G. E. GEORGES, F. SOLOMON and D. BOTSTEIN, 1987 Insertions of up to 17 amino acids into a region of α-tubulin do not disrupt function in vivo. Mol. Cell. Biol. 7: 3799-3805.
28. SHERMAN, F., G. FINK and J. HICKS, 1986 Laboratory Course Manual for Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
29. SIEGERS, K., T. WALDMANN, M. R. LEROUX, K. GREIN, A. SHEVCHENKO et al., 1999 Compartmentation of protein foldling in vivo: sequestration of non-native polypeptides by the chaperonin-GimC system. EMBO J. 18: 75-84.
30. SOLOMON, F., L. CONNELL, D. KIRKPATRICK, V. PRAITIS and B. WEINSTEIN, 1992 Methods for studying the yeast cytoskeleton, pp. 197-222 in The Cytoskeleton, edited by K. CARRAWAY and C. CARRAWAY. Oxford University Press, Oxford.
31. STEARNS, T., M. HOYT and D. BOTSTEIN, 1990 Yeast mutants sensitive to antimicrotubule drugs define the genes that affect microtubule function. Genetics 124: 251-262.
32. STERNBERG, N., 1976 A genetic analysis of bacteriophage 1 head assembly. Virology 71: 568-582.
33. TIAN, G., S. LEWIS, B. FEIERBACH, T. STEARNS, H. ROMMELAERE et al., 1997 Tubulin subunits exist in an activated conformational state generated and maintained by protein cofactors. J. Cell Biol. 138: 821-832.
34. URSIC, D., and M. CULBERTSON, 1991 The yeast homolog to mouse Tcp-1 affects microtubule-mediated processes. Mol. Cell. Biol. 11:2629-2640.
35. VAINBERG, I. E., S. A. LEWIS, H. ROMMELAERE, C. AMPE, J. VANDEKERCKHOVE et al., 1998 Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin. Cell 93: 863-873.
36. VEGA, L., J. FLEMING and F. SOLOMON, 1998 An α-tubulin mutant destabilizes the heterodimer: phenotypic consequences and interactions with tubulin-binding proteins. Mol. Biol. Cell 9: 2349-2360.
37. WEINSTEIN, B., and F. SOLOMON, 1990 Phenotypic consequences of tubulin overproduction in Saccharomyces cerevisiae: differences between alpha-tubulin and beta-tubulin. Mol. Cell. Biol. 10: 5295-5304.
38. WEISSMAN, J., Y. KASHI, W. FENTON and A. HORWICH, 1994 GroEL-mediated protein folding proceeds by multiple rounds of binding and release of nonnative forms. Cell 78: 693-702.