Cloning, expression, and characterization of a bi-functional disintegrin/alkaline phosphatase hybrid protein

Protein Expression and Purification

Volumn 31, Issue 2, 2003, Pages 286-291

  Butera, Diego ^a,b   Skielka, Karen ^a   McLane, Mary Ann ^c   Paquette Straub, Carrie ^c   Ducancel, Frédéric ^d   Moura Da Silva, Ana Maria ^a  

^a INSTITUTO BUTANTAN   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c UNIVERSITY OF DELAWARE   (United States)

^d DIF   (France)

Author keywords

Disintegrin; Eristostatin; Molecular marker; IIb 3 integrin

Indexed keywords

SERPENTES; VIPER;

EID: 0142183572     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(03)00169-4     Document Type: Article

References (25)

1. M.A. Schwartz, M.H. Ginsberg, Networks and crosstalk: integrin signalling spreads, Nat. Cell Biol. 4 (2000) 65-68.
2. L.V. Parise, Integrin α(IIb)β(3) signaling in platelet adhesion and aggregation, Curr. Opin. Cell Biol. 11 (1999) 597-601.
3. J.N. George, J.P. Caen, A.T. Nurden, Glanzmann's thrombasthenia: the spectrum of clinical disease, Blood 75 (1990) 1383-1395.
4. Y.Q. Chen, M. Trikha, X. Gao, R. Bazaz, A.T. Porter, J. Timar, K.V. Honn, Ectopic expression of platelet integrin αIIb β3 in tumor cells from various species and histological origin, Int. J. Cancer 72 (1997) 642-648.
5. M.A. McLane, C. Marcinkiewicz, S. Vijay-Kumar, I. Wierzbicka-Patynowski, S. Niewiarowski, Viper venom disintegrins and related molecules, Proc. Soc. Exp. Biol. Med. 219 (1998) 109-119.
6. T.F. Huang, What have snakes taught us about integrins?, Cell. Mol. Life Sci. 54 (1998) 527-540.
7. M.A. McLane, M.A. Kowalska, L. Silver, S.J. Shattil, S. Niewiarowski, Interaction of disintegrins with the α IIb β 3 receptor on resting and activated human platelets, Biochem. J. 301 (1994) 429-436.
8. M.A. McLane, S. Vijay-Kumar, C. Marcinkiewicz, J.J. Calvete, S. Niewiarowski, Importance of the structure of the RGD-containing loop in the disintegrins echistatin and eristostatin for recognition of α IIb β 3 and α v β 3 integrins, FEBS Lett. 391 (1996) 139-143.
9. I. Wierzbicka-Patynowski, S. Niewiarowski, C. Marcinkiewicz, J.J. Calvete, M.M. Marcinkiewicz, M.A. McLane, Structural requirements of echistatin for the recognition of α(v)β(3) and α(5)β(1) integrins, J. Biol. Chem. 274 (1999) 37809-37814.
10. J.L. Guesdon, Immunoenzymatic techniques applied to the specific detection of nucleic acids. A review, J. Immunol. Methods 150 (1992) 33-49.
11. D. Gillet, F. Ducancel, E. Pradel, M. Léonetti, A. Ménez, J.C. Boulain, Insertion of a disulfide-containing neurotoxin into E. coli alkaline phosphatase: the hybrid retains both biological activities, Protein Eng. 5 (1992) 273-278.
12. F. Ducancel, D. Gillet, A. Carrier, E. Lajeunesse, A. Menez, J.C. Boulain, Recombinant colorimetric antibodies: construction and characterization of a bifunctional F(ab)2/alkaline phosphatase conjugate produced in Escherichia coli. Biotechnology 11 (1993) 601-605.
13. C. Lindbladh, K. Mosbach, L. Bulow, Use of genetically prepared enzyme conjugates in enzyme immunoassay, Trends Biochem. Sci. 18 (1993) 279-283.
14. D. Gillet, E. Ezan, F. Ducancel, C. Gaillard, T. Ardouin, M. Istin, A. Menez, J.C. Boulain, J.M. Grognet, Enzyme immunoassay using a rat prolactin-alkaline phosphatase recombinant tracer, Anal. Chem. 65 (1993) 1779-1784.
15. C. Chanussot, L. Bellanger, C. Ligny-Lemaire, P. Seguin, A. Menez, J.C. Boulain, Engineering of a recombinant colorimetric fusion protein for immunodiagnosis of insulin, J. Immunol. Methods 197 (1996) 39-49.
16. W. Wels, I.M. Harwerth, M. Zwickl, N. Hardman, B. Groner, N. E. Hynes, Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human erbB-2 receptor, Biotechnology (NY) 10 (1992) 1128-1132.
17. A. Carrier, F. Ducancel, N.B. Settiawan, L. Cattolico, B. Maillere, M. Leonetti, P. Drevet, A. Menez, J.C. Boulain, Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection, J. Immunol. Methods 181 (1995) 177-186.
18. B.H. Muller, D. Chevrier, J.C. Boulain, J.L. Guesdon, Recombinant single-chain Fv antibody fragment-alkaline phosphatase conjugate for one step immunodetection in molecular hybridization, J. Immunological. Methods 227 (1999) 177-185.
19. K. Mérienne, N. Germain, S. Zinn-Justin, J.C. Boulain, F. Ducancel, A. Ménez, The functional architecture of an acetylcholine receptor-mimicking antibody, J. Biol. Chem. 272 (1997) 23775-23783.
20. B.H. Muller, C. Lamoure, M.H. Le Du, L. Cattolico, E. Lajeunesse, F. Lemaître, A. Pearson, F. Ducancel, A. Ménez, J.C. Boulain, Improving Escherichia coli alkaline phosphatase efficacy by additional mutations inside and outside the catalytic pocket, Chem. Biol. Chem. 2 (2001) 517-523.
21. M. Uhlen, T. Moks, Gene fusions for purpose of expression: an introduction, Methods Enzymol. 185 (1990) 129-143.
22. F. Ducancel, J.C. Boulain, O. Tremeau, A. Menez, Direct expression in E. coli of a functionally active protein A-snake toxin fusion protein, Protein Eng. 3 (1989) 139-143.
23. A.S. Kamiguti, J.R. Slupsky, M. Zuzel, C.R. Hay, Properties of fibrinogen cleaved by Jararhagin, a metalloproteinase from the venom of Bothrops jararaca, Thromb. Haemost. 72 (1994) 244-249.
24. C.A. Burtis, E.R. Ashwood, Tietz Fundamentals of Clinical Chemistry, fifth ed., Saunders, Philadelphia, PA, 2001, p. 366.
25. D.C. Martin, S.C. Pastra-Landis, E.R. Kantrowitz, Amino acid substitutions at the subunit interface of dimeric Escherichia coli alkaline phosphatase cause reduced structural stability, Protein Sci. 8 (1999) 1152-1159.