Formation of mixed glycine and GABAergic synapses in cultured spinal cord neurons

European Journal of Neuroscience

Volumn 12, Issue 11, 2000, Pages 3883-3892

  Dumoulin, A ^a   Levi S ^a   Riveau, B ^a   Gasnier, B ^b   Triller, A ^a  

^a ECOLE NORMALE SUPÉRIEURE   (France)

^b INST DE BIOLOGIE PHYSICO CHIMIQUE   (France)

Author keywords

GABA; Glycine; Rat spinal cord; Receptor clustering; Synaptogenesis; VIAAT

Indexed keywords

4 AMINOBUTYRIC ACID RECEPTOR; GEPHYRIN; GLYCINE; GLYCINE RECEPTOR;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CONTROLLED STUDY; GABAERGIC SYSTEM; NONHUMAN; POSTSYNAPTIC INHIBITION; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RAT; RAT STRAIN; RECEPTOR BINDING; SPINAL CORD NERVE CELL; SYNAPTOGENESIS;

AMINO ACID TRANSPORT SYSTEMS; ANIMALS; CARRIER PROTEINS; CELLS, CULTURED; NEURONS; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, GABA-A; RECEPTORS, GLYCINE; SPINAL CORD; SYNAPSES; TIME FACTORS; VESICULAR INHIBITORY AMINO ACID TRANSPORT PROTEINS; VESICULAR TRANSPORT PROTEINS;

EID: 0033769370     PISSN: 0953816X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1460-9568.2000.00271.x     Document Type: Article

References (57)

1. Expression of glycine receptor α-subunits and gephyrin in cultured spinal neurons
2. GABA(A)-receptor subunits associate with specific gephyrin isoforms
3. Heterogeneity of microtubule-associated protein 2 during rat brain development
4. Inhibitory neurotransmission in rat spinal cord: Co-localization of glycine- and GABA(A)-receptors at GABAergic synaptic contacts demonstrated by triple immunofluorescence staining
5. The vesicular GABA transporter, VGAT, localizes to synaptic vesicles in sets of glycinergic as well as GABAergic neurons
6. Junctional versus extrajunctional glycine and GABA (A) receptor-mediated IPSCs in identified lamina I neurons of the adult rat spinal cord
7. Gephyrin accumulates at specific plasmalemma loci during neuronal maturation in vitro
8. Localization of components of glycinergic synapses during rat spinal cord development
9. Selective clustering of glutamate and gamma-aminobutyric acid receptors opposite terminals releasing the corresponding neurotransmitters
10. Electron microscopic analysis of gamma-aminobutyric acid and glycine colocalization in rat trigeminal subnucleus caudalis
11. Presence of the vesicular inhibitory amino acid transporter in GABAergic and glycinergic synaptic terminal boutons
12. Postsynaptic clustering of major GABA(A)R subtypes requires the γ2 subunit and gephyrin
13. Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity
14. The distribution of synapsin I and synaptophysin in hippocampal neurons developing in culture
15. GABA(A)-receptor heterogeneity in the adult rat brain: Differential regional and cellular distribution of seven major subunits
16. Localization of the clustering protein gephyrin at GABAergic synapses in the main olfactory bulb of the rat
17. Monoclonal antibodies to glutamic acid decarboxylase
18. Electrical development in spinal cord cell culture
19. Corelease of two fast neurotransmitters at a central synapse
20. Clusters of GABA(A) receptors on cultured hippocampal cells correlate only partially with functional synapses
21. Variants of the receptor/channel clustering molecule gephyrin in brain: Distinct distribution patterns, developmental profiles, and proteolytic cleavages by calpain
22. Widespread expression of gephyrin, a putative receptor-tubulin linker protein, in rat brain
23. The postsynaptic localization of the glycine receptor-associated protein gephyrin is regulated by the cytoskeleton
24. Glycine-receptor activation is required for receptor clustering in spinal neurons
25. Gephyrin antisense oligonucleotides prevent glycine receptor clustering in spinal cord
26. Distribution of gephyrin transcripts in the adult and developing rat brain
27. Loss of postsynaptic GABA (A) receptor clustering in gephyrin-deficient mice
28. Synaptic vesicle dynamics in living cultured hippocampal neurons visualized with CY3-conjugated antibodies directed against the lumenal domain of synaptotagmin
29. Strychnine-sensitive stabilization of postsynaptic glycine receptor clusters
30. Synaptic control of glycine and GABA(A) receptors and gephyrin expression in cultured motoneurons
31. Transient expression of GABA immunoreactivity in the developing rat spinal cord
32. Ontogeny of GABA(A) receptor subunit mRNAs in rat spinal cord and dorsal root ganglia
33. Identification of a gephyrin binding motif on the glycine receptor subunit
34. Identification and characterization of the vesicular GABA transporter
35. Development of glycine receptor alpha subunit in cultivated rat spinal neurons: An immunocytochemical study
36. The development of excitatory synapses in cultured spinal neurons
37. Immunohistochemical evidence for coexistence of glycine and GABA in nerve terminals on cat spinal motoneurones: An ultrastructural study
38. Qualitative and quantitative analysis of glycine- and GABA-immunoreactive nerve terminals on motoneuron cell bodies in the cat spinal cord: A postembedding electron microscopic study
39. Distribution of glutamate-, glycine- and GABA-immunoreactive nerve terminals on dendrites in the cat spinal motor nucleus
40. Colocalization of glycine-like and GABA-like immunoreactivities in Golgi cell terminals in the rat cerebellum: A postembedding light and electron microscopic study
41. Monoclonal antibodies and peptide mapping reveal structure similarities between the subunits of the glycine receptor of rat spinal cord
42. Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein
43. Resolving GABA(A)/benzodiazepine receptors: Cellular and subcellular localization in the CNS with monoclonal antibodies
44. Cloning of a functional vesicular GABA and glycine transporter by screening of genome databases
45. Colocalization of gephyrin and GABA(A)-receptor subunits in the rat retina
46. - ion channels exhibit heterogenous channel properties
47. Partial glycinergic denervation induces changes in the distribution of a glycine receptor-associated protein in a central neuron
48. Immunocytochemical localization of amino acid neurotransmitter candidates in the ventral horn of the cat spinal cord: A light microscopic study
49. GABA and glycine frequently colocalize in terminals on cat spinal motoneurons
50. Light microscope study of the coexistence of GABA-like and glycine-like immunoreactivities in the spinal cord of the rat
51. Colocalization of GABA, glycine, and their receptors at synapses of the rat spinal cord
52. Gamma-aminobutyric acid-containing terminals can be apposed to glycine receptors at central synapses
53. GABA (A)-receptor-associated protein links GABA (A) receptors and the cytoskeleton
54. Glycine immunoreactivity localized in the cochlear nucleus and superior olivary complex
55. Colocalization of GABA and glycine in the rabbit oculomotor nucleus
56. Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles
57. Glycine-immunoreactive terminals in the rat trigeminal motor nucleus: Light- and electron-microscopic analysis of their relationships with motoneurones and with GABA-immunoreactive terminals